Poster (Scientific congresses and symposiums)
Establishment of an interactomic map of the Ets factors family: Towards a better understanding of their roles in oncogenic processes
Rambout, Xavier; Simonis, Nicolas; Demoitié, Pauline et al.
2011IAP Network 28 : Signal integration mechanisms in health and disease
 

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Abstract :
[en] Ets transcription factors have been involved in several cancers such as leukemia, prostate cancer and Ewing’s sarcoma. They regulate the expression of genes controlling important biological processes such as cellular proliferation, differentiation, apoptosis, metastasis, and transformation. This family of transcription factors is characterized by its highly conserved DNA-binding domain called the ETS domain and members are classified into subfamilies based on sequence homology criterion. We built a protein-protein interaction (PPI) network of the 27 Ets proteins and of their individual functional domains using a high-throughput yeast-two hybrid (Y2H) screening method. That Y2H network was expanded with confident literature-curated PPIs to obtain a comprehensive Ets interaction network. By considering connectivity between Ets interaction partners, we were able to segregate highly connected clusters of proteins from that network. Analysis of ontologies enrichment of those clusters enabled to confirm well-established roles and regulations of Ets factors, but also to suggest new ones. Biological validation of one precise cluster could be used as a rule of a thumb to globally confirm the bioinformatic analysis of our Ets PPI network and the potential physiological or pathological roles and regulation of Ets factors.
Research center :
Giga-Signal Transduction - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Rambout, Xavier ;  Université de Liège - ULiège > Chimie et bio-industries > Biologie cell. et moléc.
Simonis, Nicolas
Demoitié, Pauline
Vidal, Marc
Twizere, Jean-Claude  ;  Université de Liège - ULiège > Chimie et bio-industries > Biologie cell. et moléc.
Dequiedt, Franck  ;  Université de Liège - ULiège > GIGA-Research
Language :
English
Title :
Establishment of an interactomic map of the Ets factors family: Towards a better understanding of their roles in oncogenic processes
Publication date :
29 April 2011
Number of pages :
A1
Event name :
IAP Network 28 : Signal integration mechanisms in health and disease
Event organizer :
Rider, Mark
Event place :
Bruxelles, Belgium
Event date :
29/04/2011
Name of the research project :
Establishment of an interactomic map of the Ets factors family: Towards a better understanding of their roles in oncogenic processes
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Télévie [BE]
Fondation contre le Cancer [BE]
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since 12 March 2012

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