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Contribution à la caractérisation moléculaire des relations hôte-pathogène de plants de pêchers infectés par le viroïde de la mosaïque latente du pêcher (PLMVd)
Parisi, Olivier
2007
 

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Keywords :
viroïde; pêcher; PLMVd; AFLP
Abstract :
[en] Since several years, the Plant Pathology Unit studies the Peach latent mosaic viroid (PLMVd) and other viroids from fruit trees. These studies have led, first, to the lightening of new pseudoknot interactions potentially involved in the pathogenic mecanisms of the PLMVd. Secondly the existence of recombination events during the replication of the viroids was expressed. The first goal of this work was to create a collection of dimeric variants to be able to verify these hypothesys during the following years. Dimeric constructions of severe and latent variants were successfully obtained. Unfortunately, the variants selected to perform the recombination analysis contained too much mutations to be used. Finally two design of specific primers were performed. The first provides the possibility to create mutations in the pseudoknots and the second makes us able to detect recombinant variants. The second goal of this work was to set up the cDNA-AFLP on peach extracts to perform the caracterization of host-pathogen interactions between peach trees and the PLMVd.This will be made with collaboration of the Biochemistry and Catalityc RNA Departement of the Universtiy of Sherbrooke wich will analyze the miRNA pattern of infected and healthy plants. Based on former pathogenicity studies of differents variants performed in our Departement, the comparison of differentially expressed genes will include symptomatic and non symptomatic plants and also an healthy plant. Total RNA extracts were successfully performed and a first AFLP profile was obtained. However some steps of the manipulation need an optimization to produce reliable results.
Disciplines :
Biotechnology
Agriculture & agronomy
Author, co-author :
Parisi, Olivier ;  Université de Liège - ULiège > Sciences agronomiques > Phytopathologie
Language :
French
Title :
Contribution à la caractérisation moléculaire des relations hôte-pathogène de plants de pêchers infectés par le viroïde de la mosaïque latente du pêcher (PLMVd)
Defense date :
September 2007
Number of pages :
102
Institution :
ULiège. GxABT - Liège Université. Gembloux Agro-Bio Tech
Degree :
diplôme d'études approfondies en sciences agronomiques et ingénierie biologique
Promotor :
Jijakli, Haissam  ;  Université de Liège - ULiège > Département GxABT > Gestion durable des bio-agresseurs
President :
Paul, Roger ;  Université de Liège - ULiège > Département GxABT
Jury member :
Massart, Sébastien
du Jardin, Patrick  ;  Université de Liège - ULiège > Département GxABT > Plant Sciences
Kettmann, Richard ;  Université de Liège - ULiège > Département GxABT > Microbial, food and biobased technologies
Baudoin, Jean-Pierre ;  Université de Liège - ULiège > Département GxABT > Plant Sciences
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since 04 January 2012

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