Article (Scientific journals)
Even attenuated bovine leukemia virus proviruses can be pathogenic in sheep.
Florins, Arnaud-Francois; Gillet, Nicolas; Boxus, Mathieu et al.
2007In Journal of Virology, 81 (18), p. 10195-200
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Keywords :
Amino Acid Motifs/genetics; Animals; Cattle; Cell Transformation, Viral/genetics; DNA Mutational Analysis; Enzootic Bovine Leukosis/genetics/virology; Leukemia Virus, Bovine/genetics/pathogenicity; Lymphoma/genetics/veterinary/virology; Membrane Proteins/genetics; Sequence Deletion; Sheep/virology; Sheep Diseases/genetics/virology; Time Factors; Viral Proteins/genetics; Virus Latency/genetics
Abstract :
[en] Based on a reverse genetics approach, we previously reported that bovine leukemia virus (BLV) mutants harboring deletions in the accessory R3 and G4 genes persist at very low proviral loads and are unable to induce leukemia or lymphoma in sheep, indicating that these R3 and G4 gene sequences are required for pathogenesis. We now show that lymphoma can occur, albeit infrequently (1 case of 20) and after extended periods of latency (7 years). Direct sequencing and reinfection experiments demonstrated that lymphomagenesis was not due to the reversion of the mutant to the wild type. Similar observations with another type of attenuated mutant impaired in the transmembrane protein (TM) YXXL signaling motifs were made. We conclude that the R3 and G4 genes and the TM YXXL motifs are not strictly required for pathogenesis but that their integrity contributes to disease frequency and latency.
Disciplines :
Veterinary medicine & animal health
Oncology
Author, co-author :
Florins, Arnaud-Francois ;  Université de Liège - ULiège > Chimie et bio-industries > Centre de Bio. Fond. - Section de Biologie cell. et moléc.
Gillet, Nicolas ;  Université de Liège - ULiège > Chimie et bio-industries > Centre de Bio. Fond. - Section de Biologie cell. et moléc.
Boxus, Mathieu ;  Université de Liège - ULiège > Chimie et bio-industries > Centre de Bio. Fond. - Section de Biologie cell. et moléc.
Kerkhofs, Pierre
Kettmann, Richard ;  Université de Liège - ULiège > Chimie et bio-industries > Centre de Bio. Fond. - Section de Biologie cell. et moléc.
Willems, Luc  ;  Université de Liège - ULiège > GIGA-Research
Language :
English
Title :
Even attenuated bovine leukemia virus proviruses can be pathogenic in sheep.
Publication date :
2007
Journal title :
Journal of Virology
ISSN :
0022-538X
eISSN :
1098-5514
Publisher :
American Society for Microbiology, Washington, United States - District of Columbia
Volume :
81
Issue :
18
Pages :
10195-200
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 07 April 2009

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