Human T-cell leukemia virus type-1 Tax oncoprotein regulates G-protein signaling.

Cells, Cultured; Chemokine CXCL12; Chemokines, CXC/metabolism; Deltaretrovirus Infections; GTP-Binding Proteins/metabolism/physiology; Gene Products, tax/genetics/physiology; Humans; Receptors, CXCR4/metabolism; Receptors, G-Protein-Coupled/physiology; Signal Transduction; T-Lymphocytes; Transduction, Genetic

Abstract :

[en] Human T-cell leukemia virus type-1 (HTLV-1) is associated with adult T-cell leukemia (ATL) and neurological syndromes. HTLV-1 encodes the oncoprotein Tax-1, which modulates viral and cellular gene expression leading to T-cell transformation. Guanine nucleotide-binding proteins (G proteins) and G protein-coupled receptors (GPCRs) constitute the largest family of membrane proteins known and are involved in the regulation of most biological functions. Here, we report an interaction between HTLV-1 Tax oncoprotein and the G-protein beta subunit. Interestingly, though the G-protein beta subunit inhibits Tax-mediated viral transcription, Tax-1 perturbs G-protein beta subcellular localization. Functional evidence for these observations was obtained using conditional Tax-1-expressing transformed T-lymphocytes, where Tax expression correlated with activation of the SDF-1/CXCR4 axis. Our data indicated that HTLV-1 developed a strategy based on the activation of the SDF-1/CXCR4 axis in the infected cell; this could have tremendous implications for new therapeutic strategies.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Twizere, Jean-Claude ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech

Springael, Jean-Yves

Boxus, Mathieu ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech

Burny, Arsène ; Université de Liège - ULiège > Agro Biotech Gembloux

Dequiedt, Franck ; Université de Liège - ULiège > GIGA-Research

Dewulf, Jean-Francois

Duchateau, Julie

Portetelle, Daniel ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech

Urbain, Patrice

Van Lint, Carine

More authors (5 more)

Language :

English

Title :

Human T-cell leukemia virus type-1 Tax oncoprotein regulates G-protein signaling.

Publication date :

2007

Journal title :

Blood

ISSN :

0006-4971

eISSN :

1528-0020

Publisher :

American Society of Hematology, Washington, United States - District of Columbia

Volume :

109

Issue :

Pages :

1051-60

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 11 January 2010

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