[en] Human T-cell leukemia virus type-1 (HTLV-1) is associated with adult T-cell leukemia (ATL) and neurological syndromes. HTLV-1 encodes the oncoprotein Tax-1, which modulates viral and cellular gene expression leading to T-cell transformation. Guanine nucleotide-binding proteins (G proteins) and G protein-coupled receptors (GPCRs) constitute the largest family of membrane proteins known and are involved in the regulation of most biological functions. Here, we report an interaction between HTLV-1 Tax oncoprotein and the G-protein beta subunit. Interestingly, though the G-protein beta subunit inhibits Tax-mediated viral transcription, Tax-1 perturbs G-protein beta subcellular localization. Functional evidence for these observations was obtained using conditional Tax-1-expressing transformed T-lymphocytes, where Tax expression correlated with activation of the SDF-1/CXCR4 axis. Our data indicated that HTLV-1 developed a strategy based on the activation of the SDF-1/CXCR4 axis in the infected cell; this could have tremendous implications for new therapeutic strategies.
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Bibliography
Poiesz BJ, Ruscetti FW, Gazdar AF, et al. Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma. Proc Natl Acad Sci U S A. 1980;77:7415-7419.
Takatsuki K. Discovery of adult T-cell leukemia. Retrovirology. 2005;2:16.
Gessain A, Barin F, Vernant JC, et al. Antibodies to human T-lymphotropic virus type-I in patients with tropical spastic paraparesis. Lancet. 1985;2:407-410.
Matsuoka M, Jeang KT. Human T-cell leukemia virus type I at age 25: a progress report. Cancer Res. 2005;65:4467-4470.
Jeang KT, Giam CZ, Majone F, Aboud M. Life, death, and tax: role of HTLV-I oncoprotein in genetic instability and cellular transformation. J Biol Chem. 2004;279:31991-31994.
Grassmann R, Aboud M, Jeang KT. Molecular mechanisms of cellular transformation by HTLV-1 Tax. Oncogene. 2005;24:5976-5985.
Azran I, Schavinsky-Khrapunsky Y, Aboud M. Role of Tax protein in human T-cell leukemia virus type-I leukemogenicity. Retrovirology. 2004;1:20.
Felber BK, Paskalis H, Kleinman-Ewing C, Wong-Staal F, Pavlakis GN. The pX protein of HTLV-I is a transcriptional activator of its long terminal repeats. Science. 1985;229:675-679.
Brady J, Jeang KT, Duvall J, Khoury G. Identification of p40x-responsive regulatory sequences within the human T-cell leukemia virus type I long terminal repeat. J Virol. 1987;61:2175-2181.
Crenon I, Beraud C, Simard P, et al. The transcriptionally active factors mediating the effect of the HTLV-I Tax transactivator on the IL-2R α kappa B enhancer include the product of the c-rel proto-oncogene. Oncogene. 1993;8:867-875.
Maruyama M, Shibuya H, Harada H, et al. Evidence for aberrant activation of the interleukin-2 autocrine loop by HTLV-1-encoded p40x and T3/Ti complex triggering. Cell. 1987;48:343-350.
Azimi N, Brown K, Bamford RN, et al. Human T cell lymphotropic virus type I Tax protein transactivates interleukin 15 gene transcription through an NF-κB site. Proc Natl Acad Sci U S A. 1998;95:2452-2457.
Mariner JM, Lantz V, Waldmann TA, Azimi N. Human T cell lymphotropic virus type I Tax activates IL-15R α gene expression through an NF-kappa B site. J Immunol. 2001;166:2602-2609.
Himes SR, Coles LS, Katsikeros R, Lang RK, Shannon MF. HTLV-1 tax activation of the GMCSF and G-CSF promoters requires the interaction of NF-κB with other transcription factor families. Oncogene. 1993;8:3189-3197.
Albrecht H, Shakhov AN, Jongeneel CV. trans Activation of the tumor necrosis factor α promoter by the human T-cell leukemia virus type I Tax1 protein. J Virol. 1992;66:6191-6193.
Neuveut C, Low KG, Maldarelli F, et al. Human T-cell leukemia virus type 1 Tax and cell cycle progression: role of cyclin D-cdk and p110Rb. Mol Cell Biol. 1998;18:3620-3632.
Haller K, Ruckes T, Schmitt I, et al. Tax-dependent stimulation of G1 phase-specific cyclin-dependent kinases and increased expression of signal transduction genes characterize HTLV type 1-transformed T cells. AIDS Res Hum Retroviruses. 2000;16:1683-1688.
Haller K, Wu Y, Derow E, et al. Physical interaction of human T-cell leukemia virus type 1 Tax with cyclin-dependent kinase 4 stimulates the phosphorylation of retinoblastoma protein. Mol Cell Biol. 2002;22:3327-3338.
Santiago F, Clark E, Chong S, et al. Transcriptional up-regulation of the cyclin D2 gene and acquisition of new cyclin-dependent kinase partners in human T-cell leukemia virus type 1-infected cells. J Virol. 1999;73:9917-9927.
Suzuki T, Kitao S, Matsushime H, Yoshida M. HTLV-1 Tax protein interacts with cyclin-dependent kinase inhibitor p16INK4A and counteracts its inhibitory activity towards CDK4. EMBO J. 1996;15:1607-1614.
Jin DY, Spencer F, Jeang KT. Human T cell leukemia virus type 1 oncoprotein Tax targets the human mitotic checkpoint protein MAD1. Cell. 1998;93:81-91.
Yin MJ, Christerson LB, Yamamoto Y, et al. HTLV-I Tax protein binds to MEKK1 to stimulate IκB kinase activity and NF-κB activation. Cell. 1998;93:875-884.
Sun SC, Yamaoka S. Activation of NF-κB by HTLV-I and implications for cell transformation. Oncogene. 2005;24:5952-5964.
Jiang H, Lu H, Schiltz RL, et al. PCAF interacts with tax and stimulates tax transactivation in a histone acetyltransferase-independent manner. Mol Cell Biol. 1999;19:8136-8145.
Clapham DE, Neer EJ. G-protein β gamma subunits. Annu Rev Pharmacol Toxicol. 1997;37:167-203.
Hamm HE. The many faces of G protein signaling. J Biol Chem. 1998;273:669-672.
Stephens L, Smrcka A, Cooke FT, et al. A novel phosphoinositide 3 kinase activity in myeloid-derived cells is activated by G-protein β gamma subunits. Cell. 1994;77:83-93.
Sternweis PC, Smrcka AV. G proteins in signal transduction: the regulation of phospholipase C. Ciba Found Symp. 1993;176:96-106.
Tang WJ, Iniguez-Lluhi JA, Mumby S, Gilman AG. Regulation of mammalian adenylyl cyclases by G-protein α and β gamma subunits. Cold Spring Harb Symp Quant Biol. 1992;57:135-144.
Velimirovic BM, Gordon EA, Lim NF, Navarro B, Clapham DE. The K+ channel inward rectifier subunits form a channel similar to neuronal G protein-gated K+ channel. FEBS Lett. 1996;379:31-37.
Eichmann T, Lorenz K, Hoffmann M, et al. The amino-terminal domain of G-protein-coupled receptor kinase 2 is a regulatory Gβγ binding site. J Biol Chem. 2003;278:8052-8057.
Gao J, Li J, Chen Y, Ma L. Activation of tyrosine kinase of EGFR induces Gβγ-dependent GRK-EGFR complex formation. FEBS Lett. 2005;579:122-126.
Spiegelberg BD, Hamm HE. Gβγbinds histone deacetylase 5 (HDAC5) and inhibits its transcriptional co-repression activity. J Biol Chem. 2005;280:41769-41776.
Kino T, Tiulpakov A, Ichijo T, et al. G-protein β interacts with the glucocorticoid receptor and suppresses its transcriptional activity in the nucleus. J Cell Biol. 2005;169:885-896.
Ford CE, Skiba NP, Bae H, et al. Molecular basis for interactions of G proteinβγ subunits with effectors. Science. 1998;280:1271-1274.
Waldele K, Schneider G, Ruckes T, Grassmann R. Interleukin-13 overexpression by tax transactivation: a potential autocrine stimulus in human T-cell leukemia virus-infected lymphocytes. J Virol. 2004;78:6081-6090.
Schmitt I, Rosin O, Rohwer P, Gossen M, Grassmann R. Stimulation of cyclin-dependent kinase activity and G1- to S-phase transition in human lymphocytes by the human T-cell leukemia/lymphotropic virus type 1 Tax protein. J Virol. 1998;72:633-640.
Twizere JC, Lefebvre L, Collete D, et al. The homeobox protein MSX2 interacts with tax oncoproteins and represses their transactivation activity. J Biol Chem. 2005;280:29804-29811.
Twizere JC, Kruys V, Lefebvre L, et al. Interaction of retroviral Tax oncoproteins with tristetraprolin and regulation of tumor necrosis factor-α expression. J Natl Cancer Inst. 2003;95:1846-1859.
Meertens L, Chevalier S, Weil R, Gessain A, Mahieux R. A 10-amino acid domain within human T-cell leukemia virus type 1 and type 2 tax protein sequences is responsible for their divergent subcellular distribution. J Biol Chem. 2004;279:43307-43320.
Ross TM, Narayan M, Fang ZY, Minella AC, Green PL. Human T-cell leukemia virus type 2 tax mutants that selectively abrogate NFκB or CREB/ATF activation fail to transform primary human T cells. J Virol. 2000;74:2655-2662.
El-Asmar L, Springael JY, Ballet S, et al. Evidence for negative binding cooperativity within CCR5-CCR2b heterodimers. Mol Pharmacol. 2005;67:460-469.
Schmidt CJ, Neer EJ. In vitro synthesis of G-protein β gamma dimers. J Biol Chem. 1991;266:4538-4544.
Lambright DG, Sondek J, Bohm A, et al. The 2.0 A crystal structure of a heterotrimeric G protein. Nature. 1996;379:311-319.
Peloponese JM Jr, Haller K, Miyazato A, Jeang KT. Abnormal centrosome amplification in cells through the targeting of Ran-binding protein-1 by the human T cell leukemia virus type-1 Tax oncoprotein. Proc Natl Acad Sci U S A. 2005;102:18974-18979.
Smith MR, Greene WC. Characterization of a novel nuclear localization signal in the HTLV-I tax transactivator protein. Virology. 1992;187:316-320.
Alefantis T, Barmak K, Harhaj EW, Grant C, Wigdahl B. Characterization of a nuclear export signal within the human T cell leukemia virus type I transactivator protein Tax. J Biol Chem. 2003;278:21814-21822.
Peloponese JM Jr, Iha H, Yedavalli VR, et al. Ubiquitination of human T-cell leukemia virus type 1 tax modulates its activity. J Virol. 2004;78:11686-11695.
Chiari E, Lamsoul I, Lodewick J, et al. Stable ubiquitination of human T-cell leukemia virus type 1 tax is required for proteasome binding. J Virol. 2004;78:11823-11832.
Bex F, Murphy K, Wattiez R, Burny A, Gaynor RB. Phosphorylation of the human T-cell leukemia virus type 1 transactivator tax on adjacent serine residues is critical for tax activation. J Virol. 1999;73:738-745.
Lamsoul I, Lodewick J, Lebrun S, et al. Exclusive ubiquitination and sumoylation on overlapping lysine residues mediate NF-κB activation by the human T-cell leukemia virus tax oncoprotein. Mol Cell Biol. 2005;25:10391-10406.
Burton M, Upadhyaya CD, Maier B, Hope TJ, Semmes OJ. Human T-cell leukemia virus type 1 Tax shuttles between functionally discrete subcellular targets. J Virol. 2000;74:2351-2364.
Murphy PM. Chemokines and the molecular basis of cancer metastasis. N Engl J Med. 2001;345:833-835.
Pelchen-Matthews A, Signoret N, Klasse PJ, Fraile-Ramos A, Marsh M. Chemokine receptor trafficking and viral replication. Immunol Rev. 1999;168:33-49.
Feng Y, Broder CC, Kennedy PE, Berger EA. HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor. Science. 1996;272:872-877.
Moriuchi M, Moriuchi H, Fauci AS. HTLV type I Tax activation of the CXCR4 promoter by association with nuclear respiratory factor 1. AIDS Res Hum Retroviruses. 1999;15:821-827.
Calattini S, Chevalier SA, Duprez R, et al. Discovery of a new human T-cell lymphotropic virus (HTLV-3) in Central Africa. Retrovirology. 2005;2:30.
Chevalier SA, Meertens L, Pise-Masison C, et al. The tax protein from the primate T-cell lymphotropic virus type 3 is expressed in vivo and is functionally related to HTLV-1 Tax rather than HTLV-2 Tax. Oncogene. 2006;25:4470-4482.
Hasegawa H, Sawa H, Lewis MJ, et al. Thymusderived leukemia-lymphoma in mice transgenic for the Tax gene of human T-lymphotropic virus type I. Nat Med. 2006;12:466-472.
Harrod R, Tang Y, Nicot C, et al. An exposed KID-like domain in human T-cell lymphotropic virus type 1 Tax is responsible for the recruitment of coactivators CBP/p300. Mol Cell Biol. 1998;18:5052-5061.
Fletcher JE, Lindorfer MA, DeFilippo JM, et al. The G-protein β5 subunit interacts selectively with the Gq α subunit. J Biol Chem. 1998;273:636-644.
Jones MB, Siderovski DP, Hooks SB. The Gβγ dimer as a novel source of selectivity in G-protein signaling: GGL-ing at convention. Mol Interv. 2004;4:200-214.
Fletcher JE, Lindorfer MA, DeFilippo JM, et al. The G-protein β5 subunit interacts selectively with the Gq α subunit. J Biol Chem. 1998;273:636-644.
Watson AJ, Katz A, Simon MI. A fifth member of the mammalian G-protein β-subunit family: expression in brain and activation of the β 2 isotype of phospholipase C. J Biol Chem. 1994;269:22150-22156.
Muller DJ, De Luca V, Sicard T, et al. Suggestive association between the C825T polymorphism of the G-protein β3 subunit gene (GNB3) and clinical improvement with antipsychotics in schizophrenia. Eur Neuropsychopharmacol. 2005;15:525-531.
Smith MR, Greene WC. Characterization of a novel nuclear localization signal in the HTLV-I tax transactivator protein. Virology. 1992;187:316-320.
Alefantis T, Barmak K, Harhaj EW, Grant C, Wigdahl B. Characterization of a nuclear export signal within the human T cell leukemia virus type I transactivator protein Tax. J Biol Chem. 2003;278:21814-21822.
Burton M, Upadhyaya CD, Maier B, Hope TJ, Semmes OJ. Human T-cell leukemia virus type 1 Tax shuttles between functionally discrete subcellular targets. J Virol. 2000;74:2351-2364.
Griffin EE, Graumann J, Chan DC. The WD40 protein Caf4p is a component of the mitochondrial fission machinery and recruits Dnm1p to mitochondria. J Cell Biol. 2005;170:237-248.
Shin DH, Lee E, Chung YH, et al. Subcellular localization of WD40 repeat 1 protein in PC12 rat pheochromocytoma cells. Neurosci Lett. 2004;367:399-403.
Murray JT, Panaretou C, Stenmark H, Miaczynska M, Backer JM. Role of Rab5 in the recruitment of hVps34/p150 to the early endosome. Traffic. 2002;3:416-427.
Paulsen SJ, Rosenkilde MM, Eugen-Olsen J, Kledal TN. Epstein-Barr virus-encoded BILF1 is a constitutively active G protein-coupled receptor. J Virol. 2005;79:536-546.
Feng Y, Broder CC, Kennedy PE, Berger EA. HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor. Science. 1996;272:872-877.
Yoshida R, Nagira M, Kitaura M, et al. Secondary lymphoid-tissue chemokine is a functional ligand for the CC chemokine receptor CCR7. J Biol Chem. 1998;273:7118-7122.
Yoshie O. Expression of CCR4 in adult T-cell leukemia. Leuk Lymphoma. 2005;46:185-190.
Yoshie O, Fujisawa R, Nakayama T, et al. Frequent expression of CCR4 in adult T-cell leukemia and human T-cell leukemia virus type 1-transformed T cells. Blood. 2002;99:1505-1511.
Miyazato A, Kawakami K, Iwakura Y, Saito A. Chemokine synthesis and cellular inflammatory changes in lungs of mice bearing p40tax of human T-lymphotropic virus type 1. Clin Exp Immunol. 2000;120:113-124.
Mori N, Krensky AM, Ohshima K, et al. Elevated expression of CCL5/RANTES in adult T-cell leukemia cells: possible transactivation of the CCL5 gene by human T-cell leukemia virus type I tax. Int J Cancer. 2004;111:548-557.
Arai M, Ohashi T, Tsukahara T, et al. Human T-cell leukemia virus type 1 Tax protein induces the expression of lymphocyte chemoattractant SDF-1/PBSF. Virology. 1998;241:298-303.
Swainson L, Kinet S, Manel N, et al. Glucose transporter 1 expression identifies a population of cycling CD4+CD8+ human thymocytes with high CXCR4-induced chemotaxis. Proc Natl Acad Sci U S A. 2005;102:12867-12872.
Gales C, Rebois RV, Hogue M, et al. Real-time monitoring of receptor and G-protein interactions in living cells. Nat Methods. 2005;2:177-184.
Kenakin T. Receptor conformational induction versus selection: all part of the same energy landscape. Trends Pharmacol Sci. 1996;17:190-191.
De Lean A, Stadel JM, Lefkowitz RJ. A ternary complex model explains the agonist-specific binding properties of the adenylate cyclase-coupled β-adrenergic receptor. J Biol Chem. 1980;255:7108-7117.
Springael JY, Le Minh PN, Urizar E, et al. Allosteric modulation of binding properties between units of chemokine receptor homo- and hetero-oligomers. Mol Pharmacol. 2006;69:1652-1661.
Jin DY, Teramoto H, Giam CZ, et al. A human suppressor of c-Jun N-terminal kinase 1 activation by tumor necrosis factor α. J Biol Chem. 1997;272:25816-25823.
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