Agronomical factors influencing the legumin/ vicilin ratio in pea (Pisum sativum L.) seeds

[en] BACKGROUND: Many research studies have investigated the impact of agronomical factors on the protein content of pea (Pisum sativum). This study aimed to establish if a correlation exists between protein content and legumin/vicilin (L/V) ratio in pea seeds and to identify agronomical factors that have an impact on this ratio. RESULTS: The L/V ratio was positively correlated with protein content (r = 0.58, P ≤ 0.01), but no linear regression was applicable (adjusted R² = 0.31). Both variety and cultivation year had a highly significant effect on the ratio (P < 0.001). The interaction between these two factors was also highly significant (P < 0.001), some varieties being less sensitive to climatic conditions than others. Cultivation location had a highly significant effect (P < 0.01). There was no interaction between variety and location. Seeding density had a highly significant effect on the ratio (P < 0.01),with a saturation effect above 60 seeds/m². CONCLUSION: Further studies should establish if a linear regression model can be applied to pea varieties independently. Varieties with a stable L/V ratio can prove to be useful in the food industry. Other agronomical factors (soil type and seeding density) should be considered for the production of pea seeds with a specific L/V ratio.

Disciplines :

Agriculture & agronomy
Food science

Author, co-author :

Mertens, Cécile ; Université de Liège - ULiège > Chimie et bio-industries > Laboratoire des Sciences des aliments et formulation

Dehon, Lydie

Bourgeois, Audrey

Verhaeghe-Cartrysse, Christine; Université de Liège - ULiège > asbl APPO

Blecker, Christophe ; Université de Liège - ULiège > Chimie et bio-industries > Laboratoire des Sciences des aliments et formulation

Language :

English

Title :

Agronomical factors influencing the legumin/ vicilin ratio in pea (Pisum sativum L.) seeds

Alternative titles :

[fr] Facteurs agronomiques influençant le ratio légumine/viciline dans les graines de pois (Pisum sativum L.)

Publication date :

2012

Journal title :

Journal of the Science of Food and Agriculture

ISSN :

0022-5142

eISSN :

1097-0010

Publisher :

John Wiley & Sons, Inc, Chichester, United Kingdom

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

http://onlinelibrary.wiley.com/doi/10.1002/jsfa.4738/pdf

Available on ORBi :

since 13 December 2011

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Bibliography

Khattab RY, Arntfield SD and Nyachoti CM, Nutritional quality of legume seeds as affected by some physical treatments, Part 1: Protein quality evaluation. LWT-Food Sci Technol 42: 1107-1112 (2009).
Vidal-Valverde C, Frias J, Hernández A, Martín-Alvarez PJ, Sierra I, Rodríguez C, et al, Assessment of nutritional compounds and antinutritional factors in pea (Pisum sativum) seeds. J Sci Food Agric 83: 298-306 (2003).
Yoshida H, Tomiyama Y, Saiki M and Mizushina Y, Tocopherol content and fatty acid distribution of peas (Pisum sativum L.). J Am Oil Chem Soc 84: 1031-1038 (2007).
Martínez-Villaluenga C, Gulewicz P, Frias J, Gulewicz K and Vidal-Valverde C, Assessment of protein fractions of three cultivars of Pisum sativum L.: effect of germination. Eur Food Res Technol 226: 1465-1478 (2008).
Sathe SK, Dry bean protein functionality. Crit Rev Biotechnol 22: 175-223 (2002).
Sosulski FW and McCurdy AR, Functionality of flours, protein fractions and isolates from field peas and faba bean. J Food Sci 52: 1010-1014 (1987).
Davidsson L, Dimitriou T, Walczyk T and Hurrell RF, Iron absorption from experimental infant formulas based on pea (Pisum sativum)-protein isolate: the effect of phytic acid and ascorbic acid. Br J Nutr 85: 59-63 (2001).
Bastianelli D, Grosjean F, Peyronnet C, Duparque M and Régnier JM, Feeding value of pea (Pisum sativum L.). 1. Chemical composition of different categories of pea. Anim Sci 67: 609-619 (1998).
Dagorn-Scaviner C, Gueguen J and Lefebvre J, A comparison of interfacial behaviours of pea (Pisum sativum L.) legumin and vicilin at air/water interface. Nahrung 30: 337-447 (1986).
Guéguen J and Barbot J, Quantitative and qualitative variability of pea (Pisum sativum L.) protein composition. J Sci Food Agric 42: 209-224 (1988).
Page D and Duc G, La graine de pois, une source de protéines prometteuses. OCL 6: 518-522 (1999).
Owusuh-Ansah J and McCurdy SM, Pea proteins: a review of chemistry, technology of production and utilization. Food Rev Int 7: 103-134 (1991).
Savage GP and Deo S, The nutritional value of peas. A literature review. Nutr Abstr Rev A 59: 65-88 (1989).
Black RG, Brouwer JB, Meares C and Iyer L, Variation in physico-chemical properties of field peas (Pisum sativum). Food Res Int 31: 81-86 (1998).
Nikolopoulou D, Grigorakis K, Alexis LN, Iliadis K and Stasini M, Differences in chemical composition of field pea (Pisum sativum) cultivars: effects of cultivation area and year. Food Chem 103: 847-852 (2007).
Reichert RD and McKenzie SL, Composition of peas (Pisum sativum) varying widely in protein content. J Agric Food Chem 30: 312-317 (1982).
Wang N, Hatcher DW, Warkentin TD and Toews R, Effect of cultivar and environment on physicochemical and cooking characteristics of field pea (Pisum sativum). Food Chem 118: 109-115 (2010).
Wang N and Daun JK, Effect of variety and crude protein content on nutrients and certain antinutrients in field peas (Pisum sativum). J Sci Food Agric 84: 1021-1029 (2004).
Burstin J and Duc G, The relationship between protein content and protein composition of pea seeds. Grain Legumes 44: 16-17 (2006).
Cousin R, Peas (Pisum sativum L.). Field Crops Res 53: 111-130 (1997).
Salon C, Munier-Jonain NG, Duc G, Voisin A-S, Grandgirard D, Larmure A, et al, Grain legume seed filling in relation to nitrogen acquisition: a review and prospects with particular reference to pea. Agronomie 21: 539-552 (2001).
Schiltz S, Munier-Jolain N, Jeudy C, Burstin J and Salon C, Dynamics of exogenous nitrogen partitioning and nitrogen remobilization from vegetative organs in pea revealed by 15N in vivo labeling throughout seed filling. Plant Physiol 137: 1463-1473 (2005).
Kosson R, Czuchajowska Z and Pomeranz Y, Smooth and wrinkled peas. 1. General physical and chemical characteristics. J Agric Food Chem 42: 91-95 (1994).
Bishnoi S and Khetarpaul N, Variability in physico-chemical properties and nutrient composition of different pea cultivars. Food Chem 47: 371-373 (1993).
Eppendorfer WH and Bille SW, Amino acid composition as a function of total N (nitrogen) in pea seeds grown on soils with P (phosphorus) and K (potassium) additions. Plant Soil 41: 33-39 (1974).
Holl FB and Vose JR, Carbohydrate and protein accumulation in the developing field pea seed. Can J Plant Sci 60: 1109-1114 (1980).
McLean LA, Sosulski FW and Youngs CG, Effects of nitrogen and moisture on yield and protein in field peas. Can J Plant Sci 54: 301-305 (1974).
Singh B, Campbell WF and Salunkhe DK, Effects of s-triazines on protein and fine structure of cotyledons of bush beans. Am J Bot 59: 568-572 (1972).
Barba de la Rosa AP, Guegen J, Paredes-López O and Viroben G, Fractionation procedures, electrophoretic characterization, and amino acid composition of Amaranth seed proteins. J Agric Food Chem 40: 931-936 (1992).
Schroeder HE, Qantitative studies on the cotyledonary proteins in the genus Pisum. J Sci Food Agric 33: 623-633 (1982).
Robertson RN, Highkin HR, Smydzuk J and Went FW, The effect of environmental conditions on the development of pea. Aust J Exp Biol Med Sci 15: 1-15 (1962).
Gallardo K, Thompson R and Burstin J, Reserve accumulation in legume seeds. CR Biol 331: 755-762 (2008).
Marzo F, Aguirre A, Castiella MV and Alonso R, Fertilization effects of phosphorus and sulfur on chemical composition of seeds of Pisum sativum L. and relative infestation by Bruchus pisorum L. J Agric Food Chem 45: 1829-1833 (1997).
Al-Karaki GN and Ereifej KJ, Relationship between seed yield and chemical composition of field peas grown under semi-arid mediterranean conditions. J Agron Crop Sci 182: 279-284 (1999).
Cai T and Chang KC, Processing effect on soybean storage proteins and their relationship with tofu quality. J Agric Food Chem 47: 720-727 (1999).
Tzitzikas EN, Vincken JP, de Groot J, Gruppen H and Visser RG, Genetic variation in pea seed globulin composition. J Agric Food Chem 54: 425-433 (2006).
Kim YH, Kim SD and Hong EH, 11S and 7S globulin fractions in soybean seed and soycurd characteristics. Kor J Crop Sci 39: 348-352 (1994).
Gatehouse JA, Croy RRD, McIntosh R, Paul C and Boulter D, Quantitative and qualitative variation in the storage proteins of material from the EEC joint field bean test, in Vicia faba: Feeding Value, Processing and Viruses, ed. by Bond DA. Springer, The Hague, pp. 173-190 (1980).
Casey R, Sharman JE, Wright DJ, Bacon JR and Guldager P, Quantitative variability in Pisum seed globulins: its assessment and significance. Qual Plant Plant Foods Hum Nutr 31: 333-346 (1982).
Murphy PA and Resurreccion AP, Varietal and environmental differences in soybean glycinin and b-conglycinin content. J Agric Food Chem 32: 911-915 (1984).
Mujoo R, Trinh DT and Ng PKW, Characterization of storage proteins in different soybean varieties and their relationship to tofu yield and texture. Food Chem 82: 265-273 (2003).
Bourgeois M, Jacquin F, Savois V, Sommerer N, Labas V, Henry C, et al, Dissecting the proteome of pea mature seeds reveals the phenotypic plasticity of seed protein composition. Proteomics 9: 254-271 (2009).
Dagorn-Scaviner C, Etude des propriétés tensio-actives des globulines du pois. Application aux isolats protéiques utilisés comme agents d'émulsification ou de moussage. Thesis, UFR de Sciences, Université de Nantes (1986).
Rangel A, Domont GB, Pedrosa C and Ferreira ST, Functional properties of purified vicilins from cowpea (Vigna unguiculata) and pea (Pisum sativum) and cowpea protein isolate. J Agric Food Chem 51: 5792-5797 (2003).
Bock L, Lejeune P, Veron P, Bracke C and Bah BB, Numerical Map of the Soils of Wallonie. Agronomical Faculty of Gembloux, Gembloux (2004).
Kozhevnikov GO, Danilenko AN, Braudo EE and Schwenke KD, Comparative studies on thermodynamic characteristics of pea legumin and legumin-T thermal denaturation. Int J Biol Macromol 29: 225-236 (2001).
Gueguen J, Barbot J and Schaeffer F, Dissociation and aggregation of pea legumin induced by pH and ionic strength. J Sci Food Agric 53: 167-182 (1988).
Igbasan FA, Guenter W, Warkentin TD and McAndrew DW, Protein quality of peas as influenced by location, nitrogen application and seed inoculation. Plant Foods Hum Nutr 49: 93-105 (1996).
Pageau D, Lajeunesse L and Lafond J, Influence de la densité de semis sur la productivité du pois sec cultivé sous un climat frais. Can J Plant Sci 87: 41-48 (2007).
Blade SF, Lopetinsky K, Olson M, Laflamme P and Phillips C, High protein lupins: diversifying the pulse industry in western Canada. Proc. 4th Int. Crop Science Congr., Brisbane. (2004).
Khaliq A, Iqbal M and Basra SMA, Optimization of seeding density and nitrogen application in wheat cv Inqualab-91 under Faisalabad conditions. Int J Agric Biol 1: 241-243 (1999).