Reference : In vitro antagonistic activity evaluation of Lactic Acid Bacteria (LAB) combined with...
Scientific journals : Article
Life sciences : Biotechnology
In vitro antagonistic activity evaluation of Lactic Acid Bacteria (LAB) combined with cellulase enzyme against Campylobacter jejuni growth in co-culture
[fr] Evaluation de l'activité antagoniste de bactéties lactiques combinées avec une cellulase sur la croissance de Campylobacter
Dubois Dauphin, Robin [Université de Liège - ULiège > Chimie et bio-industries > Bio-industries >]
vandeplas, Sabrina mailto [> >]
Didderen, Isabelle [> >]
Marcq, Christopher mailto [Université de Liège - ULiège > Sciences agronomiques > Zootechnie >]
Thewis, André mailto [Université de Liège - ULiège > Sciences agronomiques > Zootechnie >]
Thonart, Philippe mailto [Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
Journal of Microbiology and Biotechnology
[en] Co-culture ; Lactic-acid bacteria ; Campylobacter ; cellulolytic enzymes ; antagonistic activity ; acetic acid
[en] The antibacterial effects of nine Lactic Acid Bacteria (LAB) on Campylobacter jejuni were investigated using agar gel diffusion and co-culture assay. Inhibition potential was not the same between both techniques. Only two LAB, Lb. pentosus CWBI B78 and E. faecium THT, showed an anti-campylobacter activity in co-culture assay in using dehydrated poultry excreta mixed with ground straw (DPE/GS) as the only growth substrate source. It was observed that the complementation with Cellulase A complex (Beldem S.A.) of this medium enhanced antimicrobial effect of both bacteria. The co-culture medium acidification was correlated with the concentration in supplemented enzyme. The antibacterial effect was characterized by the production of lactic acid by the homofermentative E. faecium THT and the lactic and acetic acids production by the heterofermentative Lb. pentosus CWBI B78. The antagonistic properties from bacteria-enzyme cooperation could reduce the prevalence of Campylobacter consequently the risk of human infection.
University of Liège, Gembloux Agro-Bio Tech - Bio-Industries Unit
Directorate for Agriculture, natural Resources, and Environment (DGARE) of the Walloon Regional Government of Belgium (Research Project, D31-1189)
Researchers ; Professionals ; Students

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