In vitro antagonistic activity evaluation of Lactic Acid Bacteria (LAB) combined with cellulase enzyme against Campylobacter jejuni growth in co-culture

Dubois Dauphin, Robin; vandeplas, Sabrina; Didderen, Isabelle; Marcq, Christopher; Thewis, André; Thonart, Philippe

doi:10.4014/jmb.1007.07006

Article (Scientific journals)

In vitro antagonistic activity evaluation of Lactic Acid Bacteria (LAB) combined with cellulase enzyme against Campylobacter jejuni growth in co-culture

Dubois Dauphin, Robin; vandeplas, Sabrina; Didderen, Isabelle et al.

2011 • In Journal of Microbiology and Biotechnology, 21 (1), p. 62-70

Peer reviewed

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https://hdl.handle.net/2268/104721

DOI
10.4014/jmb.1007.07006

PubMed
21301194

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Keywords :

Co-culture; Lactic-acid bacteria; Campylobacter; cellulolytic enzymes; antagonistic activity; acetic acid

Abstract :

[en] The antibacterial effects of nine Lactic Acid Bacteria (LAB) on Campylobacter jejuni were investigated using agar gel diffusion and co-culture assay. Inhibition potential was not the same between both techniques. Only two LAB, Lb. pentosus CWBI B78 and E. faecium THT, showed an anti-campylobacter activity in co-culture assay in using dehydrated poultry excreta mixed with ground straw (DPE/GS) as the only growth substrate source. It was observed that the complementation with Cellulase A complex (Beldem S.A.) of this medium enhanced antimicrobial effect of both bacteria. The co-culture medium acidification was correlated with the concentration in supplemented enzyme. The antibacterial effect was characterized by the production of lactic acid by the homofermentative E. faecium THT and the lactic and acetic acids production by the heterofermentative Lb. pentosus CWBI B78. The antagonistic properties from bacteria-enzyme cooperation could reduce the prevalence of Campylobacter consequently the risk of human infection.

Research Center/Unit :

University of Liège, Gembloux Agro-Bio Tech - Bio-Industries Unit

Disciplines :

Biotechnology

Author, co-author :

Dubois Dauphin, Robin ; Université de Liège - ULiège > Chimie et bio-industries > Bio-industries

vandeplas, Sabrina

Didderen, Isabelle

Marcq, Christopher ; Université de Liège - ULiège > Sciences agronomiques > Zootechnie

Thewis, André ; Université de Liège - ULiège > Sciences agronomiques > Zootechnie

Thonart, Philippe ; Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles

Language :

English

Title :

In vitro antagonistic activity evaluation of Lactic Acid Bacteria (LAB) combined with cellulase enzyme against Campylobacter jejuni growth in co-culture

Alternative titles :

[fr] Evaluation de l'activité antagoniste de bactéties lactiques combinées avec une cellulase sur la croissance de Campylobacter

Publication date :

January 2011

Journal title :

Journal of Microbiology and Biotechnology

ISSN :

1017-7825

eISSN :

1738-8872

Publisher :

Springer, Germany

Volume :

Issue :

Pages :

62-70

Peer reviewed :

Peer reviewed

Funders :

Directorate for Agriculture, natural Resources, and Environment (DGARE) of the Walloon Regional Government of Belgium (Research Project, D31-1189)

Available on ORBi :

since 06 December 2011

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