[en] A total of 678 individuals from 28 European bovine breeds were both phenotyped and analysed at the myostatin locus by the Single Strand Conformation Polymorphism (SSCP) method. Seven new mutations were identified which contribute to the high polymorphism (1 SNP every 100 bp) present in this small gene; twenty haplotypes were described and a genotyping method was set up using the Oligonucleotide Ligation Assay (OLA) method. Some haplotypes appeared to be exclusive to a particular breed; this was the case for 5 in the Charolaise (involving mutation Q204X) and 7 in the Maine-Anjou (involving mutation E226X). The relationships between the different haplotypes were studied, thus allowing to test the earlier hypothesis on the origin of muscular hypertrophy in Europe: muscular hypertrophy (namely m821 (del11)) was mainly spread in different waves from northern Europe milk purpose populations in most breeds; however, other mutations (mostly disruptive) arose in a single breed, were highly selected and have since scarcely evolved to other populations.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Dunner, S.
Miranda, M. E.
Amigues, Y.
Canon, J.
Georges, Michel ; Université de Liège - ULiège > Département de productions animales > Génomique animale
Hanset, R.
Williams, J.
Menissier, F.
Language :
English
Title :
Haplotype diversity of the myostatin gene among beef cattle breeds
Arthur P.F., Double muscling in cattle: a review, Aust. J. Agric. Res. 46 (1995) 1493-515.
Bassam B.J., Caetano-Anollés G., Gresshoff P., Fast and sensitive silver staining of DNA in polyacrylamide gels, Anal. Biochem. 196 (1991) 80-83.
Barroso A., Dunner S., Cañón J., Technical note: Detection of bovine Kappa-casein variants A, B, C and D by means of polymerase chain reaction - single strand conformation polymorphism (PCR-SSCP), J. Anim. Sci. 76 (1998) 1535-1538.
Cappucio I., Marchitelli C., Serracchioli A., Nardone A., Filippini F., Ajmone-Marsan P., Valentini A., A G-T transversion introduces a stop codon at the mh locus in hypertrophic Marchigiana beef subjects. Abstracts of the XXVIth International Conference on Animal Genetics, 9-14 August 1998, Auckland, New Zealand.
Casas E., Keele J.W., Shackelford S.D., Koohmaraie M., Sonstegard T.S., Smith T.P.L., Kappes S.M., Stone R.T., Association of the muscle hypertrophy locus with carcass traits in beef cattle, J. Anim. Sci. 76 (1998) 468-473.
Casas E., Shackelford S.D., Keele J.W., Stone R.T., Kappes S.M., Koohmaraie M., Quantitative trait loci affecting growth and carcass composition of cattle segregating alternate forms of myostatin, J. Anim. Sci. 78 (2000) 560-569.
Casas E., Stone R.T., Keele J.W., Shackelford S.D., Kappes S.M., Koohmaraie M., A comprehensive search for quantitative trait loci affecting growth and carcass composition of cattle segregating forms of the myostatin gene, J. Anim. Sci. 79 (2001) 854-860.
Charlier C., Coppieters W., Farnir F., Grobet L., Leroy P., Michaux C., Mni M., Schwers A., Vanmanshoven P., Hanset R., Georges M., The mh gene causing double-muscling in cattle maps to bovine Chromosome 2, Mamm. Genome 6 (1995) 788-792.
Dechambre, Les veaux à croupe de poulain, Rech. Méd. Vét., École Alfort, 88 (1911) 93-98.
Denis B., Regards sur la « durhamisation » des bovins au XIXe siècle. Des leçons pour aujourd'hui, Renc. Rech. Ruminants, 7 (2000) 8-18.
Dunner S., Charlier C., Farnir F., Brouwers B., Cañón J., Georges M., Towards interbreed IBD fine mapping of the mh locus: double-muscling in the Asturiana de los Valles breed involves the same locus as in the Belgian Blue cattle breed, Mamm. Genome 8 (1997) 430-435.
Ferrel R.E., Conte V., Lawrence E.C., Roth S.M., Hagberg J.M., Hurley B.F., Frequent sequence variation in the human mysotatin (GDF8) gene as a marker for analysis of muscle-related phenotypes, Genomics 62 (1999) 203-207.
González-Cadavid N., Taylor W., Yarasheski K., Sinha-hikim I., Ma K., Ezzat S., Shen R., Lalani R., Asa S., Mamita M., Nair G., Arver S., Bhasin S., Organization of the human myostatin gene and expression in healthy men and HIV-infected men with muscle wasting, Proc. Natl. Acad. Sci. USA 95 (1998) 14938-14943.
Grobet L., Royo L., Poncelet D., Pirottin D., Brouwers B., Riquet J., Schoeberlein A., Dunner S., Ménissier F., Massabanda J., Fries R., Hanset R., Georges M., A deletion in the myostatin gene causes double-muscling in cattle, Nat. Genet. 17 (1997) 71-74.
Grobet L., Poncelet D., Royo L., Brouwers B., Pirottin D., Michaux C., Ménissier F., Zanotti M., Dunner S., Georges M., Molecular definition of an allelic series of mutations disrupting the myostatin function and causing double-muscling in cattle, Mamm. Genome 9 (1998) 210-213.
Grossman P.D., Bloch W., Brinson E., Chang C.C., Eggerding F.A., Fung S., Iovannisci D.M., Woo S., Winn-Deen E.S., High-density multiplex detection of nucleic acid sequences: oligonucleotide ligation assay and sequence-coded separation, Nucl. Acids Res. 22 (1998) 4527-4534.
Hanset R., Michaux C., On the genetic determinism of muscular hypertrophy in the Belgian White and Blue cattle breed. I - Experimental data, Génét. Sél. Évol. 17 (1985a) 359-368.
Hanset R., Michaux C., On the genetic determinism of muscular hypertrophy II.-Population data, Génét. Sél. Évol. 17 (1985b) 369-386.
Jean-Pierre M., A rapid method for the purification of DNA from blood, Nucl. Acids Res. 15 (1987) p. 9611.
Kambadur R., Sharma M., Smith T.P.L., Bass J.J., Mutations in myostatin (GDF8) in double-muscled Belgian Blue Cattle, Genome Res. 7 (1997) 910-916.
Karim L., Coppieters W., Grobet L., Valentini A., Georges M., Convenient genotyping of six myostatin mutations causing double-muscling in cattle using a multiplex oligonucleotide ligation assay, Anim. Genet. 31 (2000) 396-399.
Kimura M., A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences, J. Mol. Evol. 16 (1980) 111-120.
McPherron A.C., Lee S.J., Double muscling in cattle due to mutations in the myostatin gene, Proc. Natl. Acad. Sci. USA 94 (1997) 12457-12461.
Ménissier F., Present state of knowledge about the genetic determination of muscular hypertrophy or the double muscled trait in cattle, in: King J.W.B., Ménissier F. (Eds.), Current Topics in Veterinary Medicine and Animal Science, Vol. 16: Muscle hypertrophy of genetic origin and its use to improve beef production, Martinus Nijhoff, 1982, pp. 387-428.
Miranda M.E., Royo L., García-Atance P., Cañón J., Georges M., Ménissier F., Grobet L., Bosher M.Y., Dunner S., Análisis de la heterogeneidad alélica del gen de la miostatina en diferentes razas bovinas europeas. VIII Jornadas sobre Producción Animal. Zaragoza 11-13 de mayo, ITEA 20 (1999) 288-290.
Miranda M.E., Cañón J., Ménissier F., Hanset R., Williams J., Dunner S., Identificación de los distintos haplotipos del gen de la miostatina en razas bovina europeas. (ITEA) IX Jornadas sobre Producción Animal. Volumen Extra, Número 22-Tomo I, 2001, pp. 39-41.
Miranda M.E., Amigues Y., Boscher M.Y., Ménissier F., Cortés O., Dunner S., Simultaneous genotyping to detect myostatin gene polymorphism in beef cattle breeds, J. Anim. Breed. Genet. 119 (2002) 361-366.
Orita M., Iwahana H., Kanazawa H., Hayashi K., Sekiya T., Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms, Proc. Natl. Acad. Sci. USA 86 (1998a) 2766-2770.
Orita M., Suzuki Y., Sekiya T., Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction, Genomics 5 (1998b) 874-879.
Sambrook J., Fritsch E.F., Maniatis T., Molecular cloning: a laboratory manual (2nd edn.), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989.
Simon D.L., Buchenauer D., Genetics of cattle diversity, EAAP (1993) Publication number 66.
Smith J.A., Lewis A.M., Wiener P., Williams J.L., Genetic variation in the bovine myostatin gene in UK beef cattle: Allele frequencies and haplotype analysis in the South Devon, Anim. Genet. 31 (2000a) 306-309.
Smith T.P.L., Casas E., Rexroad III C.E., Kappes S.M., Keele J.W., Bovine CAPN1 maps to a region of BTA29 containing a quantitative trait locus for meat tenderness, J. Anim. Sci. 78 (2000b) 2589-2594.
Sommer S.S., Cassady J.D., Sobell J.L., Bottema C.D.K., A novel method for detecting point mutations or polymorphisms and its application to population screening for carriers of phenylketonuria, Mayo Clin. Proc. 64 (1989) 1361-1372.
Stephens J., Schneider J., Tanguay D., et al., Haplotype variation and linkage disequilibrium in 313 genes, Science 293 (2001) 489-493.
Thierry, Les veaux à cul de poulain, J. Agric. Prat., Paris 1 (1898) 182-184.
Watterson G.A., Guess, H.A., Is the most frequent allele the oldest?, Theor. Popul. Biol. 11 (1977) 141-160.
