Positional candidate cloning of a QTL in dairy cattle: Identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition
[en] We recently mapped a quantitative trait locus (QTL) with a major effect on milk composition-particularly fat content-to the centromeric end of bovine chromosome 14. We subsequently exploited linkage disequilibrium to refine the map position of this QTL to a 3-cM chromosome interval bounded by microsatellite markers BULGE13 and BULGE09. We herein report the positional candidate cloning of this QTL, involving (I) the construction of a BAC contig spanning the corresponding marker interval, (2) the demonstration that a very strong candidate gene, acylCoA:diacylglycerol acyltransferase (DGATf), maps to that contig, and (3) the identification of a nonconservative K232A substitution in the DGAT1 gene with a major effect on milk fat content and other milk characteristics.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Grisart, Bernard ; Université de Liège - ULiège > Département de gestion vétérinaire des Ressources Animales (DRA) > GIGA-R : Génomique animale
Coppieters, Wouter ; Université de Liège - ULiège > Département de productions animales > Département de productions animales
Farnir, Frédéric ; Université de Liège - ULiège > Département de productions animales > Biostatistique, économie, sélection animale
Karim, Latifa ; Université de Liège - ULiège > Département de productions animales > Génomique animale
Ford, C.
Berzi, Paulette ; Université de Liège - ULiège > Département de gestion vétérinaire des Ressources Animales (DRA) > GIGA-R : Génomique animale
Cambisano, Nadine ; Université de Liège - ULiège > Département de productions animales > Génomique animale
Mni, Myriam ; Université de Liège - ULiège > Département de productions animales > Génomique animale
Reid, S.
Simon, Patricia ; Université de Liège - ULiège > Département de gestion vétérinaire des Ressources Animales (DRA) > GIGA-R : Génomique animale
Spelman, R.
Georges, Michel ; Université de Liège - ULiège > Département de productions animales > Génomique animale
Positional candidate cloning of a QTL in dairy cattle: Identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition
Publication date :
2002
Journal title :
Genome Research
ISSN :
1088-9051
eISSN :
1549-5469
Publisher :
Cold Spring Harbor Lab Press, Plainview, United States - Texas
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