Genetical metabolomics of flavonoid biosynthesis in Populus: a case study.

Chromatography, High Pressure Liquid/methods; Chromosome Mapping/methods; Computational Biology/methods; Flavonoids/biosynthesis/chemistry/genetics; Genes, Plant; Lod Score; Populus/chemistry/genetics/metabolism; Quantitative Trait Loci

Abstract :

[en] Genetical metabolomics [metabolite profiling combined with quantitative trait locus (QTL) analysis] has been proposed as a new tool to identify loci that control metabolite abundances. This concept was evaluated in a case study with the model tree Populus. Using HPLC, the peak abundances were analyzed of 15 closely related flavonoids present in apical tissues of two full-sib poplar families, Populus deltoides cv. S9-2 x P. nigra cv. Ghoy and P. deltoides cv. S9-2 x P. trichocarpa cv. V24, and correlation and QTL analysis were used to detect flux control points in flavonoid biosynthesis. Four robust metabolite quantitative trait loci (mQTL), associated with rate-limiting steps in flavonoid biosynthesis, were mapped. Each mQTL was involved in the flux control to one or two flavonoids. Based on the identities of the affected metabolites and the flavonoid pathway structure, a tentative function was assigned to three of these mQTL, and the corresponding candidate genes were mapped. The data indicate that the combination of metabolite profiling with QTL analysis is a valuable tool to identify control points in a complex metabolic pathway of closely related compounds.

Disciplines :

Phytobiology (plant sciences, forestry, mycology...)
Genetics & genetic processes

Author, co-author :

Morreel, Kris

Goeminne, Geert

Storme, Veronique

Sterck, Lieven

Ralph, John

Coppieters, Wouter ; Université de Liège - ULiège > Département de productions animales > Département de productions animales

Breyne, Peter

Steenackers, Marijke

Georges, Michel ; Université de Liège - ULiège > Département de productions animales > GIGA-R : Génomique animale

Messens, Eric

Boerjan, Wout

Language :

English

Title :

Genetical metabolomics of flavonoid biosynthesis in Populus: a case study.

Publication date :

2006

Journal title :

Plant Journal

ISSN :

0960-7412

eISSN :

1365-313X

Publisher :

Blackwell Publishing, Oxford, United Kingdom

Volume :

Issue :

Pages :

224-37

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 08 November 2011

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Scopus citations^®

118

Scopus citations^®
without self-citations

102

OpenCitations

105

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