[en] The viability, cytolysis and apoptosis-mediated
cellular death induced by giganteosides D and E (Gig-D and
Gig-E) and hederacolchisides A and A1 (Hcol-A and Hcol-
A1) were analysed in HL-60 cells. Materials and Methods: the
end-point metabolic (WST1) and lactate dehydrogenase
(LDH) assays were used. Cell cycle analysis and apoptosis
were measured by flow cytometry, DNA laddering and caspase
3 analyses. Results: the HL-60 cell line was more sensitive to
Hcol-A1 and Gig-D (IC50 3-5 ÌM) than to Gig-E and Hcol-A
(IC50 8-13 ÌM; WST1 assay). This was related to LDH
release. The induction of apoptosis could be detected without
caspase 3 activation after 24 h of treatment. DNA
fragmentation could be detected only with Gig-D. With Hcol-
A1 and Gig-D, an accumulation of cells in the S-phase and
an increase of cells in sub-G1 peak were observed. By the
annexinV-fluorescein isothiocyanate (FITC)/7-aminoactinomycin
D (AAD) assay, the majority of cells were in late
apoptosis with Gig-D, and in necrosis with Hcol-A1.
Conclusion: Hcol-A1 is more cytotoxic than Gig-D, followed
by Gig-E and finally Hcol-A. This is related to a membrane
permeabilization effect, leading to cytolysis
Research center :
Giga-Cancer - ULiège CART - Centre Interfacultaire d'Analyse des Résidus en Traces - ULiège
Dobson, Rowan ; Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)
tabatadze, Nino
Mshviladzade, Vakhtang
Elias, Riad
Peulen, Olivier ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Jolois, Olivier ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
De Pauw-Gillet, Marie-Claire ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie - Cytologie - Département des sciences biomédicales et précliniques
Language :
English
Title :
Apoptosis and cytolysis induced by giganteosides and hederacolchisides in HL-60 cells
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