Article (Scientific journals)
Identification and subcellular distribution of endogenous Ins(1,4,5)P3 3-kinase B in mouse tissues
Hascakova-Bartova, Romana; Pouillon, Valérie; Dewaste, Valérie et al.
2004In Biochemical and Biophysical Research Communications, 323 (3), p. 920-925
Peer reviewed
 

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Keywords :
Inositol phosphate kinase; Inositol trisphosphate; Ca2+ release; Signal transduction
Abstract :
[en] Inositol 1,4,5-trisphosphate 3-kinase (IP3-3K) catalyses the phosphorylation of inositol 1,4,5-trisphosphate to inositol 1,3,4,5-tetrakisphosphate. cDNAs encoding three mammalian isoforms have been reported and referred to as IP3-3KA, IP3-3KB, and IP3-3KC. IP3-3KB is particularly sensitive to proteolysis at the N-terminus, a mechanism known to generate active fragments of lower molecular mass. Endogenous IP3-3KB has therefore not been formally identified in tissues. We have probed a series of murine tissues with an antibody directed against the C-terminus of IP3-3KB and used IP3-3KB deficient mouse tissues as negative controls. IP3-3KB was shown to be particularly well expressed in brain, lung, and thymus with molecular masses of 110–120 kDa. The identification of the native IP3-3KB by Western blotting for the first time will facilitate further studies of regulation of its activity by specific proteases and/or phosphorylation
Disciplines :
Biochemistry, biophysics & molecular biology
Genetics & genetic processes
Author, co-author :
Hascakova-Bartova, Romana;  Université Libre de Bruxelles - ULB > Interdisciplinary Research Institute (IRIBHM)
Pouillon, Valérie;  Université Libre de Bruxelles - ULB > Interdisciplinary Research Institute (IRIBHM)
Dewaste, Valérie;  Université Libre de Bruxelles - ULB > Interdisciplinary Research Institute (IRIBHM)
Moreau, Colette;  Université Libre de Bruxelles - ULB > Interdisciplinary Research Institute (IRIBHM)
Jacques, Céline;  Université Libre de Bruxelles - ULB > IRIBHM, IBMM
Banting, George;  University of Bristol, UK > Department of Biochemistry
Schurmans, Stéphane  ;  Université Libre de Bruxelles - ULB > Institut de Recherche Interdisciplinaire en Biologie Humaine et Moleculaire
Erneux, Christophe;  Université Libre de Bruxelles - ULB > Interdisciplinary Research Institute (IRIBHM)
Language :
English
Title :
Identification and subcellular distribution of endogenous Ins(1,4,5)P3 3-kinase B in mouse tissues
Publication date :
2004
Journal title :
Biochemical and Biophysical Research Communications
ISSN :
0006-291X
eISSN :
1090-2104
Publisher :
Academic Press, San Diego, United States - California
Volume :
323
Issue :
3
Pages :
920-925
Peer reviewed :
Peer reviewed
Name of the research project :
ARC - Actions de Recherche Concertées; IAP - Interuniversity Attraction Poles
Funders :
FRSM - Fonds de la Recherche Scientifique Médicale [BE]
FWB - Fédération Wallonie-Bruxelles [BE]
BELSPO - Politique scientifique fédérale [BE]
ULB - Université Libre de Bruxelles [BE]
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