[en] Prostaglandin D2 (PGD2) receptor CRTH2, is a pro-inflammatory molecule involved in eosinophil recruitment to the allergic airway. We investigated the expression of CRTH2 in eosinophil from allergic rhinitis patients (AR) and tested the modulatory role of several TH1 and TH2 cytokines closely related to the allergic immunological response, on the expression of CRTH2 receptor, utilizing human eosinophil cell line (Eol-1).The expression of CRTH2 was tested by immunohistochemistry and flow cytometry (FACS). Chemotaxis was performed in micro-chemotaxis chambers. It is shown that the expression of CRTH2 by eosinophils was significantly higher in the nasal tissue and peripheral blood of AR patients, when compared to control subjects. PGD2 exhibited a typical bell shape dose response in attracting eosinophil from AR patients with optimal activity at 10(-7)M. Eol-1 cell surface expression of CRTH2 was significantly up-regulated by 10ng/ml IFN-gamma and TNF-alpha. The percentage of Eol-1 cells expressing the receptor increased by IFN-gamma and TNF-alpha from 12.74%+/-2.66 to 55%+/-8 and 33.8%+/-9.4, respectively. PGD2-induced Eol-1 chemotaxis was not blocked by SB203580, H-89 Dihydrochloride, Bisindo-lylmaleimide, or Genistein. PGD2-induced Eol-1 chemotaxis was potentiated by IFN-gamma and TNF-alpha without changing the signal transduction pathway. Correlation of our results to peripheral blood eosinophils from allergic rhinitis patients confirmed that 3hour pretreatment of eosinophils by 10ng/ml IFN-gamma and TNF-alpha, increased the mean fluorescence intensity (MFI) of CRTH2 from 8.23 to 9.68 and 9.38, respectively, and potentiated PGD2-induced eosinophil chemotaxis. Our results demonstrate a novel synergism between PGD2, IFN-gamma and TNF-alpha, in eosinophil chemotaxis.
Disciplines :
Otolaryngology
Author, co-author :
EL SHAZLY, Amr ; Centre Hospitalier Universitaire de Liège - CHU > O.R.L.
MOONEN, Vincent ; Centre Hospitalier Universitaire de Liège - CHU > O.R.L.
MAWET, Marie
Begon, Dominique ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques - Pathologie Expérimentale
HENKET, Monique ; Centre Hospitalier Universitaire de Liège - CHU > Pneumologie-Allergologie
Arafa, M.; Université de Liège - ULiège > Département des sciences cliniques > Pneumologie - Allergologie
LOUIS, Renaud ; Centre Hospitalier Universitaire de Liège - CHU > Pneumologie-Allergologie
Delvenne, Philippe ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
LEFEBVRE, Philippe ; Centre Hospitalier Universitaire de Liège - CHU > O.R.L.
Language :
English
Title :
IFN-gamma and TNF-alpha potentiate prostaglandin D2-induced human eosinophil chemotaxis through up-regulation of CRTH2 surface receptor.
Publication date :
2011
Journal title :
International Immunopharmacology
ISSN :
1567-5769
eISSN :
1878-1705
Publisher :
Elsevier, Netherlands
Volume :
11
Issue :
11
Pages :
1864-70
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright (c) 2011 Elsevier B.V. All rights reserved.
S. O'Sullivan, A. Roquet, B. Dahlen, S. Dahlen, and M. Kumlin Urinary excretion of inflammatory mediators during allergen-induced early and late phase asthmatic reactions Clin Exp Allergy 28 1998 1332 1339 (Pubitemid 28559006)
G. Bochenek, E. Nizankowska, A. Gieliez, M. Swierczynska, and A. Szxeklik Plasma 9 alpha,11B-PGF2, a PGD2 metabolite as a sensitive marker of mast cell activation by allergen in bronchial asthma Thorax 59 2004 459 464 (Pubitemid 38797516)
S. Narumiya, Y. Sugimoto, and F. Ushikubi Prostanoid receptors: structures, properties, and functions Physiol Rev 79 1999 1193 1226 (Pubitemid 29473315)
F. Nantel, C. Fong, S. Lamontagne, D.H. Wright, A. Giaid, and M. Desrosiers Expression of prostaglandin D synthase and the prostaglandin D2 receptors DP and CRTH2 in human nasal mucosa Prostaglandins Other Lipid Mediat 73 2004 87 101 (Pubitemid 38450719)
M. Okano, T. Fujiwara, Y. Sugata, D. Gotoh, Y. Masaoka, and M. Sogo Presence and characterization of prostaglandin D2-related molecules in nasal mucosa of patients with allergic rhinitis Am J Rhinol 20 2006 342 348 (Pubitemid 44083419)
H. Hirai, K. Tanaka, O. Yoshie, K. Ogawa, K. Kemmostu, and Y. Takamori Prostaglandin D2 selectively induces chemotaxis in T helper type 2 cells, eosinophils, and basophils via 7 transmembrane receptor CRTH2 J Exp Med 193 2001 255 261 (Pubitemid 32524457)
Y. Shiraishi, K. Asano, T. Nakajima, T. Oguma, Y. Suzuki, and T. Shiomi Prostaglandin D2-induced eosinophilic airway inflammation is mediated by CRTH2 receptor J Pharmacol Exp Ther 312 2005 954 960 (Pubitemid 40293753)
J.F. Royer, P. Schratl, S. Lorenz, E. Kostenis, T. Ulven, and R. Schuligoi A novel antagonist of CRTH2 blocks eosinophil release from bone marrow, chemotaxis and respiratory burst Allergy 62 2007 1401 1409 (Pubitemid 350045581)
L. Uller, J.M. Mathiesen, L. Alenmyr, M. Korsgren, T. Ulven, and T. Hogberg Antagonism of the prostaglandin D2 receptor CRTH2 attenuates asthma pathology in mouse eosinophilic airway inflammation Respir Res 8 2007 16
R. Nomiya, M. Okano, T. Fujiwara, M. Maeda, Y. Kimura, and K. Kino CRTH2 plays an essential role in the pathophysiology of Cry j 1-induced pollinosis in mice J Immunol 180 2008 5680 5688
E.A.B. Kelly, C.J. Koziol-White, K.C. Clay, L.K. Lui, M.E. Batis, and P.J. Bertics Potential contribution of IL-7 to allergen-induced eosinophilic airway inflammation in asthma J Immunol 182 2009 1404 1410
Inhibition of eosinophilic inflammation in allergen-challenged, IL-1 receptor type 1-deficient mice is associated with reduced eosinophil rolling and adhesion on vascular endothelium Blood 95 2000 263 269
A. El-Shazly, and P.P. Lefebvre Modulation of NK cell autocrine-induced eosinophil chemotaxis by interleukin-15 and vitamin D 3: a possible NK-eosinophil crosstalk via IL-8 in the pathophysiology of allergic rhinitis Med Inflamm 2011 (article ID 373589)
J. Choi, Z. Callaway, H.B. Kim, T. Fujisawa, and C.K. Kim The role of TNF α in eosinophilic inflammation associated with RSV bronchiolitis Pediatr Allergy Immunol 21 2009 474 479
D.H. Broid, G. Stachnick, D. Castaneda, J. Nayar, and P. Sriramarao Inhibition of eosinophilic inflammation in allergen-challenged TNF receptor p55-deficient mice Am J Respir Cell Mol Biol 24 2001 304 311 (Pubitemid 32228378)
N.W. Lukacs, R.M. Strieter, S.W. Chensue, M. Widmer, and S.L. Kunkel TNF-alpha mediates recruitment of neutrophils and eosinophils during airway inflammation J Immunol 154 1995 5411 5417
G.G. Brusselle, J.C. Kips, R.A. Pelevnan, G.F. Joos, R.R. Devos, and J.H. Tavernier Role of IFN-γ in the inhibition of the allergic airway inflammation caused by IL-12 Am J Respir Cell Mol Biol 17 1997 767 771 (Pubitemid 127348668)
L. Cohn, C. Herrick, N. Niu, R. Homer, and K. Bottomly IL-4 promotes airway eosinophilia by suppressing IFN-gamma production: defining a novel role for IFN-gamma in the regulation of allergic airway inflammation J Immunol 166 2001 2760 2767 (Pubitemid 32175128)
M. Lampinen, L. Hakansson, and P. Venge Interleukin-2 inhibits eosinophil migration but is counteracted by IL-5 priming Clin Exp Allergy 31 2001 249 258 (Pubitemid 32231858)
Kenji Ishihara, Aki Takahashi, Motoko Kaneko Hiroki Sugeno, Noriyasu Hirasawa, JangJa Hong, and Okpyo Zee Differentiation of eosinophilic leukemia Eol-1 cells into eosinophils induced by histone deacetylase inhibitors Life Sci 80 2007 1213 1220 (Pubitemid 46329010)
