Reference : Expression of alternansucrase in potato plants
Scientific journals : Article
Life sciences : Biotechnology
Expression of alternansucrase in potato plants
Jacon, Géraldine mailto [Université de Liège - ULiège > > Interface Entreprises-Université >]
Vincken, Jean-Paul [ > > ]
Suurs, Luc [ > > ]
Wang, Denong [ > > ]
Liu, Shaoyi [ > > ]
Visser, Richard [ > > ]
Biotechnology Letters
Kluwer Academic Publishers
Yes (verified by ORBi)
The Netherlands
[en] starch ; alternansucrase ; alternan ; transgenic potato ; glucansucrase ; polymer solubility
[en] Alternan, which consists of alternating alpha(1 - 3)/alpha (1 - 6)-linked glucosyl residues, was produced in potato tubers by expressing a mature alternansucrase (Asr) gene from Leuconostoc mesenteroides NRRL B-1355 in potato. Detection of alternan was performed by enzyme-linked immunosorbent assay in tuber juices, revealing a concentration between 0.3 and 1.2 mg g–1 fresh wt. The Asr transcript levels correlated well with alternan accumulation in tuber juices. It appeared that the expression of sucrose-regulated starch- synthesizing genes (ADP-glucose pyrophosphorylase subunit S and granule-bound starch synthase I) was down-regulated. Despite this, the physico- chemical properties of the transgenic starches were unaltered. These results are compared to those obtained with other transgenic potato plants producing mutan [alpha (1 - 3)-linked glucosyl residues and dextran [alpha (1 - 6)-linked glucosyl residues].
Researchers ; Professionals ; Students

File(s) associated to this reference

Fulltext file(s):

Restricted access
expression of alternansucrase in potato plants.pdfPublisher postprint211.39 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.