Reference : Oxidative Processes in Human Promonocytic Cells (Thp-1) after Differentiation into Ma...
Scientific journals : Article
Human health sciences : Laboratory medicine & medical technology
Oxidative Processes in Human Promonocytic Cells (Thp-1) after Differentiation into Macrophages by Incubation with Chlamydia Pneumoniae Extracts
Mouithys-Mickalad, Ange mailto [Université de Liège - ULiège > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
Deby-Dupont, Ginette [> > > >]
Nys, Monique mailto [Centre Hospitalier Universitaire de Liège - CHU > > Soins intensifs >]
Lamy, Maurice mailto [Université de Liège - ULiège > Département des sciences cliniques > Anesthésie et réanimation]
Deby, Carol [> >]
Biochemical and Biophysical Research Communications
Academic Press
Yes (verified by ORBi)
San Diego
[en] Human monocytes differentiated into macrophages by Chlamydia pneumoniae were able to oxidize blood lipoproteins, as discovered by Kalayoglu et al. (1998). Using a model of human promonocytic cells (THP-1), the cells were differentiated into macrophages by preincubation with C. pneumoniae extract, and further stimulated by phorbol myristate acetate. In these conditions, the differentiated cells oxidized a thiol compound and released superoxide anion as demonstrated respectively by gas liquid chromatography and electron spin resonance. The thiol oxidation and superoxide anion release were inhibited by diphenyliodonium, a NADPH oxidase and NOsynthase inhibitor, proving that the respiratory burst and the NOsynthase were involved in the oxidation processes occurring in the differentiated THP-1. The role of H(2)O(2) (derived from superoxide anion) was indicated by the enhancing effect of a peroxidase on the thiol oxidation. The presence of alpha-tocopherol in the surrounding medium strongly diminished the oxidation of the thiol target.

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