Reference : Purification de la [beta]-(1-4)-N-acétylhexosaminidase sécrétée par Streptomyces albu...
Scientific journals : Article
Life sciences : Microbiology
Purification de la [beta]-(1-4)-N-acétylhexosaminidase sécrétée par Streptomyces albus G et active sur les parois de Micrococus lysodeikticus
[en] Purification of beta-(1-4)-N-acetylhexosaminidase secreted by Streptomyces albus G and active on the cell walls of Micrococcus lysodeikticus.
Dierickx, L. [Centre National de Production et d'Etude des Substances d'origine Microbienne > > > > >]
Ghuysen, Jean-Marie [Université de Liège - ULiège > Laboratoire de Microbiologie Générale et Médicale > > > >]
Biochimica et Biophysica Acta
Yes (verified by ORBi)
[en] amidohydrolases ; amino sugars ; micrococcus ; streptomyces ; chemistry ; metabolism
[en] The N-acetylhexosaminidase (referred as Str) secreted by Streptomyces albus G. has been purified by zone electrophoresis. It is slightly less basic than egg-white lysozyme. Its isoelectric point is close to pH 10.3. It has no action on the [alpha]- and [beta]-phenylglycosides of N-acetylglucosamine and on the disaccharides [beta]-(1-4)-di-N-acetylglucosamine (di-N-acetylchitobiose) and [beta]-(1-6)-N-acetylglucosaminyl-N-acetylmuramic acid but it splits the tetrasaccharide [beta]-(1-4)-tetra-N-acetylglucosamine(tetra-N-acetylchitotetraose) in di-N-acetylchitobiose. Contrary to lysozyme, it does not split the tetrasaccharide O-[beta]-N-acetylglucosaminyl-(1-6)-O-[beta]-N-acetylmuraminyl-(1-4)-O-[beta]-N-acetylglucosaminyl-(1-6)-[beta]-N-acetylmuramic acid in disaccharide by hydrolyzing the [beta](1-4) linkage. In those conditions the disaccharide liberated, as well as the tetrasaccharide, from Microccus lysodeikticus cell walls after incubation with the N-acetylhexosaminidase Str, can not be put down to a further digestion of some tetrasaccharidic fragments. A chitobiase only active on the [beta]-phenyl-glycoside of N-acetylglucosamine and on the di-N-acetylchitobiose is also present in contentrated culture filtrates. It is a protein acid at pH 6.6.
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