Redefining the role of psr in beta-lactam resistance and cell autolysis of Enterococcus hirae.

Anti-Bacterial Agents/pharmacology; Bacterial Proteins/genetics/metabolism; Bacteriolysis; Base Sequence; Carrier Proteins/genetics/metabolism; Enterococcus/drug effects/genetics/growth & development/physiology; Gene Expression Regulation, Bacterial; Hexosyltransferases/genetics/metabolism; Microbial Sensitivity Tests; Molecular Sequence Data; Muramoylpentapeptide Carboxypeptidase/genetics/metabolism; Mutation; Penicillin-Binding Proteins; Peptidyl Transferases/genetics/metabolism; Recombination, Genetic; Repressor Proteins/genetics/metabolism; Sequence Analysis, DNA; beta-Lactam Resistance

Abstract :

[en] The contribution of penicillin-binding protein 5 (PBP5) and the PBP5 synthesis repressor (Psr) to the beta-lactam resistance, growth, and cell autolysis of wild-type strain ATCC 9790 and resistant strain R40 of Enterococcus hirae was investigated by disruption or substitution of the corresponding pbp5 and psr genes by Campbell-type recombination. The resulting modifications were confirmed by hybridization and PCR. The low susceptibility of E. hirae to beta-lactams was confirmed to be largely dependent on the presence of PBP5. However, against all expectations, inactivation of psr in ATCC 9790 or complementation of R40 cells with psr did not modify the susceptibility to benzylpenicillin or the growth and cell autolysis rates. These results indicated that the psr gene does not seem to be involved in the regulation of PBP5 synthesis and consequently in beta-lactam resistance or in the regulation of cell autolysis in E. hirae.

Disciplines :

Microbiology

Author, co-author :

Sapunaric, Frédéric

Franssen, Christine

Stefanic, Patrick; Université de Liège - ULiège > Centre d'ingénierie des protéines

Amoroso, Ana Maria

Dardenne, Olivier; Université de Liège - ULiège > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences)

Coyette, Jacques

Language :

English

Title :

Redefining the role of psr in beta-lactam resistance and cell autolysis of Enterococcus hirae.

Publication date :

2003

Journal title :

Journal of Bacteriology

ISSN :

0021-9193

eISSN :

1098-5530

Publisher :

American Society for Microbiology (ASM), Washington, United States

Volume :

185

Issue :

Pages :

5925-35

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 07 July 2011

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