d(CGGTGGT) forms an octameric parallel G-quadruplex via stacking of unusual G(:C):G(:C):G(:C):G(:C) octads

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Keywords :

mass spectrometry; nucleic acids; non-covalent; G-quadruplex; electrospray; FTICR

Abstract :

[en] Among non-canonical DNA secondary structures, G-quadruplexes are currently widely studied because of their probable involvement in many pivotal biological roles, and for their potential use in nanotechnology. The overall quadruplex scaffold can exhibit several morphologies through intramolecular or intermolecular organization of G-rich oligodeoxyribonucleic acid strands. In particular, several G-rich strands can form higher order assemblies by multimerization between several G-quadruplex units. Here, we report on the identification of a novel dimerization pathway. Our Nuclear magnetic resonance, circular dichroism, UV, gel electrophoresis and mass spectrometry studies on the DNA sequence dCGGTGGT demonstrate that this sequence forms an octamer when annealed in presence of K+ or NH4+ ions, through the 5′-5′ stacking of two tetramolecular G-quadruplex subunits via unusual G(:C):G(:C):G(:C):G(:C) octads.

Research center :

Giga-Systems Biology and Chemical Biology - ULiège

Disciplines :

Chemistry
Biochemistry, biophysics & molecular biology

Author, co-author :

Borbone, Nicola; University of Naples Federico II, 80131 Naples, Italy

Amato, Jussara; University of Naples Federico II, 80131 Naples, Italy

Oliviero, Giorgia; University of Naples Federico II, 80131 Naples, Italy

D'Atri, Valentina; University of Naples Federico II, 80131 Naples, Italy

Gabelica, Valérie ; Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)

De Pauw, Edwin ; Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)

Piccialli, Gennaro; University of Naples Federico II, 80131 Naples, Italy

Mayol, Luciano; University of Naples Federico II, 80131 Naples, Italy

Language :

English

Title :

d(CGGTGGT) forms an octameric parallel G-quadruplex via stacking of unusual G(:C):G(:C):G(:C):G(:C) octads

Publication date :

2011

Journal title :

Nucleic Acids Research

ISSN :

0305-1048

eISSN :

1362-4962

Publisher :

Oxford University Press, Oxford, United Kingdom

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]

Available on ORBi :

since 04 July 2011

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