Reference : Hydrolysis of starch by sorghum malt for maltodextrin production
Scientific congresses and symposiums : Poster
Life sciences : Biotechnology
Hydrolysis of starch by sorghum malt for maltodextrin production
Ba, Khady mailto [Université de Liège - ULiège > > > Doct. sc. agro. & ingé. biol.]
Destain, Jacqueline mailto [Université de Liège - ULiège > Chimie et bio-industries > Bio-industries >]
Thonart, Philippe mailto [Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
2e Journée de réflexion de l'EDT GEPROC : Génie des procédés appliqué aux bio-industries.
16 Décembre 2009
Frank Delvigne, Philippe Thonart, Michel Crine
[en] Sorghum amylase, enzymatic hydrolysis, maltodextrin, dextrose equivalent.
[en] Maltodextrin is a mixture of saccharides with a molecular weight between polysaccharides and oligosaccharides with DE lower than 20. Maltodextrin is more soluble in water than native starches, also is cheaper in comparison with other major edible hydrocolloids. Maltodextrin is obtained by moderate enzymatic or acidic hydrolysis of starch. The hydrolysis of starch, catalyzed by amylases, is the most important commercial enzyme process. The hydrolyzed products are widely applied in food, paper and textile industries. Because of the increasing demand for these enzymes in various industries, there is enormous interest in developing enzymes with better properties, such as raw starch-degrading amylases suitable for industrial
applications and their cost-effective production techniques. Sorghum (Sorghum bicolor) is a widely grown crop in Africa. Obtaining enzymes from sorghum requires a transformation. The objective of this study was application of sorghum amylase for maltodextrin. Sorghum seeds were supplied by the ISRA (Bambey, Senegal). Seeds were germinated in the laboratory at
30°C for 72 h and the sorghum malt was dried at 40°C for 48 h. Corn starch (from Roquette, France) hydrolysis was assayed in a bioreactor of 2 l at a temperature of 65°C gently stirred. Raw starch was slurried in water (30% w/v) and sorghum malt was introduced, chloride calcium was added, pH was adjusted to 6. Maltodextrin was characterized in term of the dextrose
equivalent (DE) during the hydrolysis. The yield of hydrolysis was evaluated by soluble solids (° BRIX) at different hydrolysis time. The glucose concentration released was measured by DNS method (Miller method).
Centre Wallon de Biologie Industrielle
Wallonie Bruxelles Internationale
Contribution à l'étude des amylases du sorgho et leur utilisation dans la transformation des produits amylacés
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