Poster (Scientific congresses and symposiums)
Spatio-temporal localization of the cytoskeleton during auditory organ development in mammalia
Johnen, Nicolas; Thelen, Nicolas; Cloes, Marie et al.
2011Cellular cytoskeletal motor proteins
 

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Keywords :
Auditory Organ; Cytoskeleton; Development
Abstract :
[en] The auditory organ, the organ of Corti (OC), is a highly specialized structure composed by specific cellular types. The sensory cells (HC) are characterized by stereocilia at their apex and are necessary for the sound perception. Theses cells are supported by supporting cells. Based on their morphology and physiology, at least four types of supporting cells (SC) can be identified in the OC: inner and outer pillar cells (PC), phalangeal cell and Deiter’s cells. Sensory and supporting cells possess characteristic cytoskeleton proteins in direct relation with their morphological features and their development. Indeed, this organ had morphological changes such as the setting up of the sensory epithelium after the birth or the openings of the Corti’s tunnel at P8 and of the Nuel’s spaces at P10. In the present study, by using confocal microscopy, we investigated the spatio-temporal localization of the three cellular cytoskeletal filaments : microtubules (β-1, 2, 3, 4-tubulin), microfilaments (cytoplasmic β- and γ-actin) and intermediate filaments (CK4, 5, 7, 8, CKpan and vimentin) during the development of the OC in rat from the embryonic day 18 (E18) to the post-natal day 25 (P25). The immunolabellings indicated clearly that β-1, 2, 3-tubulins were only present the SC and nervous fibers during development whereas β-4-tubulin was found firstly in the HC and then in the SC. The two actin-isotypes were detected in the HC apex but were also seen in the PC from P8 to P25 for β-actin isoform and in the basal membrane from E18 to P8 for the γ-actin isoform. All intermediate filament proteins were only found in the SC, especially between P8 and P12. Our results show that the localization of the cytoskeleton proteins during the auditory organ development depends on the cellular type and the developmental stage. A profound modification of cytoskeleton occurs between P8 and P12.
Research center :
Giga-Neurosciences - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Johnen, Nicolas ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire
Thelen, Nicolas  ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire
Cloes, Marie ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire
Malgrange, Brigitte  ;  Université de Liège - ULiège > GIGA - Neurosciences
Thiry, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire
Language :
English
Title :
Spatio-temporal localization of the cytoskeleton during auditory organ development in mammalia
Alternative titles :
[en] Localisation du cytosquelette au sein des cellules de soutien durant le dévelopemment de l'organe de Corti chez les mammifères
Publication date :
31 March 2011
Number of pages :
A0
Event name :
Cellular cytoskeletal motor proteins
Event organizer :
Biochemical Society / Wellcome Trust Focused Meeting -
Event place :
Cambridge, Hinxton, United Kingdom
Event date :
du 30 mars 2011 au 1 avril 2011
Audience :
International
Name of the research project :
Spatio-temporal localization of the cytoskeleton during auditory organ development in mammalia.
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Available on ORBi :
since 26 May 2011

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