Importance of the E-46-D-160 Polypeptide Segment of the Non-Penicillin-Binding Module for the Folding of the Low-Affinity, Multimodular Class B Penicillin-Binding Protein 5 of Enterococcus Hirae
Mollerach, Marta E.; Partoune, Pierre; Coyette, Jacqueset al.
1996 • In Journal of Bacteriology, 178 (6), p. 1774-1775
[en] Compared with the other class B multimodular penicillin- binding proteins (PBPs), the low-affinity PBP5 responsible for penicillin resistance in Enterococcus hirae R40, has an extended non-penicillin-binding module because of the presence of an approximately 110-amino-acid E-46(-)D-160 insert downstream from the membrane anchor. Expression of pbp5 genes lacking various parts of the insert-encoding region gives rise to proteins that are inert in terms of penicillin binding, showing that during folding of the PBP, the insert plays a role in the acquisition of a correct penicillin-binding configuration by the G-364(-)Q-678 carboxy-terminal module.
Research Center/Unit :
CIP - Centre d'Ingénierie des Protéines - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Mollerach, Marta E.; Madrid > Departamento de Microbiologia Molecular > Centro de Investigaciones Biologicas
Partoune, Pierre; Madrid > Departamento de Microbiologia Molecular > Centro de Investigaciones Biologicas
Coyette, Jacques ; Université de Liège - ULiège > Institut de Chimie > Centre d'Ingénierie des Protéines
Ghuysen, Jean-Marie ; Université de Liège - ULiège > Institut de Chimie > Centre d'Ingénierie des Protéines
Language :
English
Title :
Importance of the E-46-D-160 Polypeptide Segment of the Non-Penicillin-Binding Module for the Folding of the Low-Affinity, Multimodular Class B Penicillin-Binding Protein 5 of Enterococcus Hirae
Publication date :
March 1996
Journal title :
Journal of Bacteriology
ISSN :
0021-9193
eISSN :
1098-5530
Publisher :
American Society for Microbiology (ASM), Washington, United States - District of Columbia
Volume :
178
Issue :
6
Pages :
1774-1775
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FRSM - Fonds de la Recherche Scientifique Médicale
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