Article (Scientific journals)
Isolation of a Microsporum canis gene family encoding three subtilisin-like proteases expressed in vivo
Descamps, Frédéric; Brouta, Frédéric; Monod, Michel et al.
2002In Journal of Investigative Dermatology, 119 (4), p. 830-835
Peer Reviewed verified by ORBi
 

Files


Full Text
2002-Isolation of a Mc gene family encoding three subtilisin-like proteases.pdf
Publisher postprint (188.56 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
dermatophyte; keratinase; subtilisin-like protease
Abstract :
[en] Microsporum canis is the main agent of dermatophytosis in dogs and cats and is responsible for frequent zoonosis. The pathogenesis of the disease remains largely unknown, however. Among potential fungal virulence factors are secreted keratinolytic proteases, whose molecular characterization would be an important step towards the understanding of dermatophytic infection pathogenesis. M. canis secretes a 31.5 kDa keratinolytic subtilisin-like protease as the major component in a culture medium containing cat keratin as the sole nitrogen source. Using a probe corresponding to a gene's internal fragment, which was obtained by polymerase chain reaction, the entire gene encoding this protease named SUB3 was cloned from a M. canis lambdaEMBL3 genomic library. Two closely related genes, termed SUB1 and SUB2, were also cloned from the library using as a probe the gene coding for Aspergillus fumigatus 33 kDa alkaline protease (ALP). Deduced amino acid sequence analysis revealed that SUB1, SUB2, and SUB3 are secreted proteases and show large regions of identity between themselves and with subtilisin-like proteases of other filamentous fungi. Interestingly, mRNA of SUB1, SUB2, and SUBS were detected by reverse transcriptase nested-polymerase chain reaction from hair of experimentally infected guinea pigs. These results show that SUB1, SUB2, and SUB3 encode a family of subtilisin-like proteases and strongly suggest that these proteases are produced by M. canis during the invasion of keratinized structures. This is the first report describing the isolation of a gene family encoding potential virulence-related factors in dermatophytes.
Disciplines :
Veterinary medicine & animal health
Dermatology
Author, co-author :
Descamps, Frédéric
Brouta, Frédéric
Monod, Michel
Zaugg, C.
Baar, Didier
Losson, Bertrand ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires
Mignon, Bernard ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires
Language :
English
Title :
Isolation of a Microsporum canis gene family encoding three subtilisin-like proteases expressed in vivo
Publication date :
October 2002
Journal title :
Journal of Investigative Dermatology
ISSN :
0022-202X
eISSN :
1523-1747
Publisher :
Blackwell Publishing Inc, Malden, United States - Massachusetts
Volume :
119
Issue :
4
Pages :
830-835
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 17 March 2009

Statistics


Number of views
82 (6 by ULiège)
Number of downloads
2 (2 by ULiège)

Scopus citations®
 
65
Scopus citations®
without self-citations
45
OpenCitations
 
54

Bibliography


Similar publications



Contact ORBi