Recombinant expression and antigenic properties of a 31.5-kDa keratinolytic subtilisin-like serine protease from Microsporum canis

Descamps, Frédéric; Brouta, Frédéric; Vermout, Sandy; Monod, Michel; Losson, Bertrand; Mignon, Bernard

doi:10.1016/S0928-8244(03)00101-9

Article (Scientific journals)

Recombinant expression and antigenic properties of a 31.5-kDa keratinolytic subtilisin-like serine protease from Microsporum canis

Descamps, Frédéric; Brouta, Frédéric; Vermout, Sandy et al.

2003 • In FEMS Immunology and Medical Microbiology, 38 (1), p. 29-34

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/9071

DOI
10.1016/S0928-8244(03)00101-9

PubMed
12900052

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Keywords :

keratinase; subtilisin-like protease; ELISA; lymphoproliferation test; Microsporum canis; Pichia pastoris

Abstract :

[en] A secreted 31.5-kDa keratinolytic subtilase (SUB3; AJ431180) is thought to be a Microsporum canis virulence factor and represents a candidate for vaccination trials. In this study, the recombinant keratinase (r-SUB3) was produced by the Pichia pastoris expression system and purified to homogeneity. Recombinant SUB3 displayed identical biochemical properties with the native protease. Experimentally cutaneously infected guinea pigs showed specific lymphoproliferative response towards r-SUB3, while no specific humoral immune response was induced except for one animal. The heterologous expression of SUB3 provides a valuable tool for addressing further investigations on the role of this keratinase in the specific cellular immune response and on its use in vaccination trials in the cat. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

Disciplines :

Immunology & infectious disease

Author, co-author :

Descamps, Frédéric

Brouta, Frédéric

Vermout, Sandy

Monod, Michel

Losson, Bertrand ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires

Mignon, Bernard ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires

Language :

English

Title :

Recombinant expression and antigenic properties of a 31.5-kDa keratinolytic subtilisin-like serine protease from Microsporum canis

Publication date :

18 August 2003

Journal title :

FEMS Immunology and Medical Microbiology

ISSN :

0928-8244

Publisher :

Elsevier Science Bv, Amsterdam, Netherlands

Volume :

Issue :

Pages :

29-34

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 17 March 2009

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