Article (Scientific journals)
Recombinant expression and antigenic properties of a 31.5-kDa keratinolytic subtilisin-like serine protease from Microsporum canis
Descamps, Frédéric; Brouta, Frédéric; Vermout, Sandy et al.
2003In FEMS Immunology and Medical Microbiology, 38 (1), p. 29-34
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Keywords :
keratinase; subtilisin-like protease; ELISA; lymphoproliferation test; Microsporum canis; Pichia pastoris
Abstract :
[en] A secreted 31.5-kDa keratinolytic subtilase (SUB3; AJ431180) is thought to be a Microsporum canis virulence factor and represents a candidate for vaccination trials. In this study, the recombinant keratinase (r-SUB3) was produced by the Pichia pastoris expression system and purified to homogeneity. Recombinant SUB3 displayed identical biochemical properties with the native protease. Experimentally cutaneously infected guinea pigs showed specific lymphoproliferative response towards r-SUB3, while no specific humoral immune response was induced except for one animal. The heterologous expression of SUB3 provides a valuable tool for addressing further investigations on the role of this keratinase in the specific cellular immune response and on its use in vaccination trials in the cat. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
Disciplines :
Immunology & infectious disease
Author, co-author :
Descamps, Frédéric
Brouta, Frédéric
Vermout, Sandy
Monod, Michel
Losson, Bertrand ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires
Mignon, Bernard ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires
Language :
English
Title :
Recombinant expression and antigenic properties of a 31.5-kDa keratinolytic subtilisin-like serine protease from Microsporum canis
Publication date :
18 August 2003
Journal title :
FEMS Immunology and Medical Microbiology
ISSN :
0928-8244
Publisher :
Elsevier Science Bv, Amsterdam, Netherlands
Volume :
38
Issue :
1
Pages :
29-34
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 17 March 2009

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