Isolation, pure culture and characterization of Serratia symbiotica, the R-type of secondary endosymbionts of the black bean aphid Aphis fabae.

Sabri, Ahmed; Leroy, Pascal; Haubruge, Eric; Hance, Thierry; Frere, Isabelle; Destain, Jacqueline; Thonart, Philippe

doi:10.1099/ijs.0.024133-0

Article (Scientific journals)

Isolation, pure culture and characterization of Serratia symbiotica, the R-type of secondary endosymbionts of the black bean aphid Aphis fabae.

Sabri, Ahmed; Leroy, Pascal; Haubruge, Eric et al.

2010 • In International Journal of Systematic and Evolutionary Microbiology

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/90622

DOI
10.1099/ijs.0.024133-0

PubMed
20870890

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Keywords :

Serratia symbiotica; Aphids; Symbionts; Aphis fabae

Abstract :

[en] An intracellular symbiotic bacterium was isolated from the flora of a natural clone of the black bean aphid Aphis fabae. The strain was able to grow freely in aerobic condition on a rich medium containing 1% of each of following substrates; dextrose, yeast extract and peptone of casein. Pure culture was achieved through the use of solid-phase culture on the same medium and was designated CWBI-2.3T. 16S ribosomal DNA sequence analysis revealed that CWBI-2.3T strain was a member of the gamma-3 subgroup of the class Proteobacteria, having high sequence identity (>99%) with the R-Type of secondary endosymbiont found in several aphid species Serratia symbiotica. As strain CWBI-2.3T (=LMG 25624T =DSM 23270T) was the first R-type symbiont to be isolated and characterized, we proposed to designate it as the type strain of Serratia symbiotica species.

Research Center/Unit :

Centre Wallon de Biologie Industrielle

Disciplines :

Microbiology

Author, co-author :

Sabri, Ahmed ; Université de Liège - ULiège > Centre Wallon de Biologie Industrielle (CWBI)

Leroy, Pascal ; Université de Liège - ULiège > Department of Functional and Evolutionary Entomology

Haubruge, Eric ; Université de Liège - ULiège > Department of Functional and Evolutionary Entomology > Vice-Recteur de Gembloux Agro Bio Tech

Hance, Thierry; Université Catholique de Louvain - UCL > Earth and Life Institute, Biodiversity research Center

Frere, Isabelle; Université Catholique de Louvain - UCL > Earth and Life Institute, Biodiversity research Center

Destain, Jacqueline ; Université de Liège - ULiège > Centre Wallon de Biologie Industrielle (CWBI)

Thonart, Philippe ; Université de Liège - ULiège > Centre Wallon de Biologie Industrielle (CWBI)

Language :

English

Title :

Isolation, pure culture and characterization of Serratia symbiotica, the R-type of secondary endosymbionts of the black bean aphid Aphis fabae.

Publication date :

2010

Journal title :

International Journal of Systematic and Evolutionary Microbiology

ISSN :

1466-5026

eISSN :

1466-5034

Publisher :

Society for General Microbiology, Reading, United Kingdom

Peer reviewed :

Peer Reviewed verified by ORBi

Name of the research project :

SOLAPHID

Funders :

Région Wallonne de Belgique

Commentary :

This work was supported by "la Région Wallonne de Belgique" convention Waleo2 253 n°061/6288.

Available on ORBi :

since 10 May 2011

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