[en] An intracellular symbiotic bacterium was isolated from the flora of a natural clone of the black bean aphid Aphis fabae. The strain was able to grow freely in aerobic condition on a rich medium containing 1% of each of following substrates; dextrose, yeast extract and peptone of casein. Pure culture was achieved through the use of solid-phase culture on the same medium and was designated CWBI-2.3T. 16S ribosomal DNA sequence analysis revealed that CWBI-2.3T strain was a member of the gamma-3 subgroup of the class Proteobacteria, having high sequence identity (>99%) with the R-Type of secondary endosymbiont found in several aphid species Serratia symbiotica. As strain CWBI-2.3T (=LMG 25624T =DSM 23270T) was the first R-type symbiont to be isolated and characterized, we proposed to designate it as the type strain of Serratia symbiotica species.
Research center :
Centre Wallon de Biologie Industrielle
Disciplines :
Microbiology
Author, co-author :
Sabri, Ahmed ; Université de Liège - ULiège > Centre Wallon de Biologie Industrielle (CWBI)
Leroy, Pascal ; Université de Liège - ULiège > Department of Functional and Evolutionary Entomology
Haubruge, Eric ; Université de Liège - ULiège > Department of Functional and Evolutionary Entomology > Vice-Recteur de Gembloux Agro Bio Tech
Hance, Thierry; Université Catholique de Louvain - UCL > Earth and Life Institute, Biodiversity research Center
Frere, Isabelle; Université Catholique de Louvain - UCL > Earth and Life Institute, Biodiversity research Center
Destain, Jacqueline ; Université de Liège - ULiège > Centre Wallon de Biologie Industrielle (CWBI)
Thonart, Philippe ; Université de Liège - ULiège > Centre Wallon de Biologie Industrielle (CWBI)
Language :
English
Title :
Isolation, pure culture and characterization of Serratia symbiotica, the R-type of secondary endosymbionts of the black bean aphid Aphis fabae.
Publication date :
2010
Journal title :
International Journal of Systematic and Evolutionary Microbiology
ISSN :
1466-5026
eISSN :
1466-5034
Publisher :
Society for General Microbiology, Reading, United Kingdom
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
SOLAPHID
Funders :
Région Wallonne de Belgique
Commentary :
This work was supported by "la Région Wallonne de Belgique" convention Waleo2
253 n°061/6288.
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