Article (Scientific journals)
Secreted dipeptidyl peptidases as potential virulence factors for Microsporum canis.
Vermout, Sandy; Baldo, Aline; Tabart, Jeremy et al.
2008In FEMS Immunology and Medical Microbiology, 54 (3), p. 299-308
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Keywords :
Amino Acid Sequence; Animals; Dermatomycoses/microbiology/pathology; Dipeptidyl Peptidases/genetics/metabolism/secretion; Dogs; Female; Guinea Pigs; Hypersensitivity, Delayed; Microsporum/enzymology/genetics/pathogenicity; Molecular Sequence Data; Specific Pathogen-Free Organisms; Virulence; Virulence Factors/genetics/metabolism
Abstract :
[en] Dermatophytoses caused by Microsporum canis are frequently encountered in cats and dogs; they are highly contagious and readily transmissible to humans. In this study, two single genes, respectively coding for dipeptidyl peptidases IV and V (DppIV and DppV), were isolated and characterized. Both proteins share homology with serine proteases of the S9 family, some of which display properties compatible with implication in pathogenic processes. Both genes are expressed in vivo in experimentally infected guinea-pigs and in naturally infected cats, and when the fungus is grown on extracellular matrix proteins as the sole nitrogen and carbon source. DppIV and V were produced as active recombinant proteases in the yeast Pichia pastoris; the apparent molecular weight of rDppV is 83 kDa, whereas rDppIV appears as a doublet of 95 and 98 kDa. Like other members of its enzymatic subfamily, rDppIV has an unusual ability to cleave Pro-X bonds. This activity does not enhance the solubilization of keratin by fungal secreted endoproteases, and the protease probably acts solely on small soluble peptides. RDppV showed no ability to induce delayed-type hypersensitivity (DTH) skin reactions in guinea-pigs, despite the known immunogenic properties of homologous proteins.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Vermout, Sandy
Baldo, Aline ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires
Tabart, Jeremy
Losson, Bertrand ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires
Mignon, Bernard ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires
Language :
French
Title :
Secreted dipeptidyl peptidases as potential virulence factors for Microsporum canis.
Publication date :
2008
Journal title :
FEMS Immunology and Medical Microbiology
ISSN :
0928-8244
Publisher :
Blackwell Publishing, Oxford, United Kingdom
Volume :
54
Issue :
3
Pages :
299-308
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 17 March 2009

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