[en] The Src homology 2-containing inositol phosphatase SHIP1 functions in hemopoietic cells to limit activation events mediated by PI3K products, including Akt activation and cell survival. In contrast to the limited cellular expression of SHIP1, the related isoform SHIP2, is widely expressed in both parenchymal and hemopoietic cells. The goal of this study was to determine how SHIP2 functions to regulate M-CSF signaling. We report that 1) SHIP2 was tyrosine-phosphorylated in M-CSF-stimulated human alveolar macrophages, human THP-1 cells, murine macrophages, and the murine macrophage cell line RAW264; 2) SHIP2 associated with the M-CSF receptor after M-CSF stimulation; and 3) SHIP2 associated with the actin-binding protein filamin and localization to the cell membrane, requiring the proline-rich domain, but not on the Src homology 2 domain of SHIP2. Analyzing the function of SHIP2 in M-CSF-stimulated cells by expressing either wild-type SHIP2 or an Src homology 2 domain mutant of SHIP2 reduced Akt activation in response to M-CSF stimulation. In contrast, the expression of a catalytically deficient mutant of SHIP2 or the proline-rich domain of SHIP2 enhanced Akt activation. Similarly, the expression of wild-type SHIP2 inhibited NF-B-mediated gene transcription. Finally, fetal liver-derived macrophages from SHIP2 gene knockout mice enhanced activation of Akt in response to M-CSF treatment. These data suggest a novel regulatory role for SHIP2 in M-CSF-stimulated myeloid cells
Wang, Y.; Ohio State University, Columbus, OH 43210 > Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, and The Dorothy M. Davis Heart and Lung Research Institute,
Keogh, R. J.; Ohio State University, Columbus, OH 43210 > Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, and The Dorothy M. Davis Heart and Lung Research Institute
Hunter, M. G.; Ohio State University, Columbus, OH 43210 > Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, and The Dorothy M. Davis Heart and Lung Research Institute
Mitchell, C. A.; Monash University, Clayton, Victoria, Australia > Department of Biochemistry and Molecular Biology
Frey, R. S.; University of Illinois College of Medicine, Chicago, IL 60612 > Department of Pharmacology
Javaid, K.; University of Illinois College of Medicine, Chicago, IL 60612 > ‡Department of Pharmacology
Malik, A. B.; University of Illinois College of Medicine, Chicago, IL 60612 > Department of Pharmacology
Schurmans, Stéphane ; Université Libre de Bruxelles - ULB > Institut de Recherche Interdisciplinaire en Biologie Humaine et Moleculaire
Tridandapani, S.; Ohio State University, Columbus, OH 43210 > Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, and The Dorothy M. Davis Heart and Lung Research Institute
Marsh, C. B.; Ohio State University, Columbus, OH 43210 > Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, and The Dorothy M. Davis Heart and Lung Research Institute
Language :
English
Title :
SHIP2 is recruited to the cell membrane upon M-CSF stimulation and regulates M-CSF-induced signaling
Publication date :
2004
Journal title :
Journal of Immunology
ISSN :
0022-1767
eISSN :
1550-6606
Publisher :
American Association of Immunologists, Baltimore, United States - Maryland
Pages :
6820-6830
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
National Institutes of Health Grants RO1HL63800, RO1HL67176, RO1HL66108, and P01HL070294, The Kelly Clark Memorial Fund, a Johnie Walker Murphy Career Investigator Award, and American Lung Association grants (to C.B.M.). S.T. is a fellow of the Leukemia and Lymphoma Society
Commentary :
Abbreviations used in this paper: PTEN, phosphatase and tensin homolog deleted from chromosome 10; Abl, Ableson tyrosine kinase; BMM, bone marrow-derived macrophage; GSK3, glycogen synthase kinase-3; HA, hemagglutinin; PI-3,4-P2, phosphatidylinositol 3,4,-disphosphate; PI-3,4,5-P3, phosphatidylinositol 3,4,5-trisphosphate; PRD, proline-rich domain; SH2, Src homology 2; WCL, whole cell lysate.
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