Reference : Normal thyroid structure and function in rhophilin 2-deficient mice
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Life sciences : Genetics & genetic processes
http://hdl.handle.net/2268/9029
Normal thyroid structure and function in rhophilin 2-deficient mice
English
Behrends, J. [Université Libre de Bruxelles - ULB > Institut de Recherches Interdisciplinaires en Biologie Humaine et Moléculaire, Faculté de Médecine > > >]
Clement, S. [Université Libre de Bruxelles - ULB > Institut de Recherches Interdisciplinaires en Biologie Humaine et Moléculaire, Faculté de Médecine > > >]
Pajak, B. [Université Libre de Bruxelles - ULB > Institut de Biologie et de Médecine Moléculaires > > >]
Pohl, V. [Université Libre de Bruxelles - ULB > Institut de Recherches Interdisciplinaires en Biologie Humaine et Moléculaire, Faculté de Médecine > > >]
Maenhaut, C. [Université Libre de Bruxelles - ULB > Institut de Recherches Interdisciplinaires en Biologie Humaine et Moléculaire, Faculté de Médecine > > >]
Dumont, J. E. [Université Libre de Bruxelles - ULB > Institut de Recherches Interdisciplinaires en Biologie Humaine et Moléculaire, Faculté de Médecine > > >]
Schurmans, Stéphane mailto [Université Libre de Bruxelles - ULB > Institut de Recherche Interdisciplinaire en Biologie Humaine et Moleculaire > > >]
2005
Molecular and Cellular Biology
American Society for Microbiology (ASM)
25
2846-2852
Yes (verified by ORBi)
International
0270-7306
1098-5549
Washington
DC
[en] Thyroid ; Function ; Rhophilin 2-Deficient Mice
[en] Rhophilin 2 is a Rho GTPase binding protein initially isolated by differential screening of a chronically thyrotropin (TSH)-stimulated dog thyroid cDNA library. In thyroid cell culture, expression of rhophilin 2 mRNA and protein is enhanced following TSH stimulation of the cyclic AMP (cAMP) transduction cascade. Yeast two-hybrid screening and coimmunoprecipitation have revealed that the GTP-bound form of RhoB and components of the cytoskeleton are protein partners of rhophilin 2. These results led us to suggest that rhophilin 2 could play an important role downstream of RhoB in the control of endocytosis during the thyroid secretory process which follows stimulation of the TSH/cAMP pathway. To validate this hypothesis, we generated rhophilin 2-deficient mice and analyzed their thyroid structure and function. Mice lacking rhophilin 2 develop normally, have normal life spans, and are fertile. They have no visible goiter and no obvious clinical signs of hyper- or hypothyroidism. The morphology of thyroid cells and follicles in these mice were normal, as were the different biological tests performed to investigate thyroid function. Our results indicate that rhophilin 2 does not play an essential role in thyroid physiology
EU Biomed 2 grant to J.E.D., the Fonds de la Recherche Scientifique Me´dicale de Belgique and the Belgian Program on Interuniversity Poles of Attraction initiated by the Belgian State, J.B. was supported by Deutsche Forschungsgemeinschaft grant BE 2156. S.C. and S.S. are, respectively, postdoctoral researcher and research director of the Belgian Fonds National de la Recherche Scientifique
Researchers ; Professionals
http://hdl.handle.net/2268/9029
10.1128/MCB.25.7.2846-2852.2005

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