Reference : Topoisomerase I-mediated DNA relaxation as a tool to study intercalation of small mol...
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Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/86616
Topoisomerase I-mediated DNA relaxation as a tool to study intercalation of small molecules into supercoiled DNA.
English
Peixoto, Paul mailto [> > > >]
Bailly, Christian [> > > >]
David-Cordonnier, Marie Hélène [> >]
2010
Drug-DNA Interaction Protocols
Springer
235-56
Yes
United States
[en] Bacteria/cytology/genetics ; Cell Death ; Cesium/pharmacology ; Chlorides/pharmacology ; DNA Topoisomerases, Type I/metabolism ; DNA, Superhelical/chemistry/genetics/isolation & purification/metabolism ; Electrophoresis, Agar Gel ; Ethidium/metabolism/pharmacology ; Genome, Bacterial/genetics ; Humans ; Intercalating Agents/metabolism/pharmacology ; Nucleic Acid Amplification Techniques ; Plasmids/genetics/isolation & purification
[en] Several biochemical and biophysical methods are available to study the intercalation of a small molecule between two consecutive base pairs of DNA. Among them, the topoisomerase I-mediated DNA relaxation assay has proved highly efficient, relatively easy to handle and very informative to investigate drug binding to DNA. The test relies on the use of a supercoiled plasmid to mimic the topological constraints of genomic DNA. The three main components of the assay - the topoisomerase I enzyme, DNA helix and intercalating small molecules - are presented here in a structural context. The principle of the assay is described in detail, along with a typical experimental protocol.
Researchers
http://hdl.handle.net/2268/86616
10.1007/978-1-60327-418-0_15

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