Article (Scientific journals)
Radioimmunoassay of Substance P in Lung Perfusate
Nemmar, A.; Gustin, Pascal; Delaunois, Annie et al.
1998In Journal of Pharmacological and Toxicological Methods, 39 (2), p. 109-115
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Keywords :
Tyrosine/diagnostic use; Substance P/pharmacokinetics; Sensitivity and Specificity; Reproducibility of Results; Radioimmunoassay/methods; Rabbits; Perfusion/methods; Lung/drug effects/metabolism; Capsaicin/pharmacology; Animals
Abstract :
[en] A radioimmunoassay of the undecapeptide substance P (SP) and its application in rabbit lung superfusate has been developed. The assay was based on the use of a radioactive tracer, 125I-SP-Tyr8, which showed, with an excess of antibodies, a specific binding greater than 90% and a nonspecific binding lower than 2%. This tracer was stable for 4 weeks at -20 degrees C. Its specific radioactivity was 384 Ci/mmol. The assay's lower limit of detection was 10 pg/ml (0.7 fmol). The determination of SP in rabbit lung perfusate required extraction and concentration using octadecylsilane cartridges. Under these conditions, the recovery of SP from experiments on lung perfusates (n=4) was 75.0+/-4.5%, and the intraassay and interassay coefficients of variation were 7.3+/-1.9% and 10.7+/-1.3%, respectively. Amounts of SP released in rabbit lung perfusates were determined following stimulation of pulmonary C fibers with capsaicin (10(-4) M). During the stimulation period, the values of SP from lung perfusates (n=6) increased significantly when compared with baseline values.
Disciplines :
Pharmacy, pharmacology & toxicology
Veterinary medicine & animal health
Author, co-author :
Nemmar, A.;  Université de Liège - ULiège
Gustin, Pascal ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Pharmacologie, pharmacothérapie et toxicologie
Delaunois, Annie ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Beckers, Jean-François  ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Sulon, Joseph ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Language :
English
Title :
Radioimmunoassay of Substance P in Lung Perfusate
Publication date :
March 1998
Journal title :
Journal of Pharmacological and Toxicological Methods
ISSN :
1056-8719
eISSN :
1873-488X
Publisher :
Elsevier Science
Volume :
39
Issue :
2
Pages :
109-115
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 12 March 2009

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