Pregnancy-associated Glycoprotein Profile during the First Trimester of Pregnancy in Egyptian Buffalo Cows.

[en] Pregnancy-associated glycoprotein (PAG) concentrations were measured in buffalo cows starting from day 28 after breeding. Oestrus was synchronized in 10 buffaloes using two injections of 25 mg prostraglandin (PG)F(2alpha) (Lutalyse((R))) at a 11-day interval. Blood sampling was conducted nearly twice weekly. Results indicated that plasma PAG concentrations in non-pregnant buffaloes were low (<0.20 ng/ml) during the whole experimental period (day 28 to 103), while in pregnant animals plasma PAG levels increased from day 28 (4.48 +/- 0.92 ng/ml) until day 41 (27.27 +/- 6.74 ng/ml), remaining high (20.71 +/- 9.20 ng/ml) until day 103. Progesterone levels were significantly (p < 0.0001) higher in pregnant (3.51-4.80 ng/ml) than in non-pregnant buffaloes (0.28-1.52 ng/ml). A significant difference (p < 0.0001) in plasma PAG concentrations between pregnant and non-pregnant animals starting at day 28 after breeding suggests that PAG-radioimmunoassay could be suitable for pregnancy diagnosis in buffaloes during this period. In conclusion, PAG test offers the advantages that it requires a single plasma sample for early pregnancy diagnosis as well as the accuracy of the test for the detection of pregnancy as early as day 28.

Disciplines :

Veterinary medicine & animal health

Author, co-author :

El-Battawy, K. A.

Melo de Sousa, Noelita ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction

Szenci, Otto

Beckers, Jean-François ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction

Language :

English

Title :

Pregnancy-associated Glycoprotein Profile during the First Trimester of Pregnancy in Egyptian Buffalo Cows.

Publication date :

April 2009

Journal title :

Reproduction in Domestic Animals

ISSN :

0936-6768

eISSN :

1439-0531

Publisher :

Blackwell Publishing, Berlin, Germany

Volume :

Issue :

Pages :

161–166

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 12 March 2009

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Bibliography

Abraham GE, 1981 : The Application of Natural Steroid Radioimmunoassay to Gynaecologic Endocrinology. Radioassay Systems in Clinical Endocrinology. Marcel Dekker, Basel.
Arora RC, Pandey RS, 1982 : Changes in peripheral plasma concentrations of progesterone, estradiol-17β and luteinizing hormone during pregnancy and around parturition in the buffalo (Bubalus bubalis). Gen Comp Endocr 48, 403 410.
Arora RC, Bachlaus NK, Prasad A, Pandey RS, 1979 : Plasma progesterone levels in buffalo heifers during first trimester of pregnancy. Indian J Exp Biol 17, 832 833.
Ayad A, Sousa NM, Sulon J, Iguer-Ouada M, Beckers JF, 2007 : Comparison of five radioimmunoassay systems for PAG measurement: ability to detect early pregnancy in cows. Reprod Dom Anim 42, 433 440.
Bage R, 2003 : Conception rates after AI in Swedish red and white dairy heifers: relationship with progesterone concentrations at AI. Reprod Dom Anim 38, 199 203.
Beal WE, Perry RC, Corah Lr, 1992 : The use of ultrasound in monitoring reproductive physiology of beef cattle. J Anim Sci 70, 924 929.
Brito LFC, Satrapa R, Marson EP, Kastelic JP, 2002 : Efficacy of PGF 2α to synchronize estrus in water buffalo cows (Bubalus bubalis) is dependent upon plasma progesterone concentration, corpus luteum size and ovarian follicular status before treatment. Anim Reprod Sci 73, 23 35.
Butler JE, Hamilton WC, Sasser RG, Ruder CA, Hass GM, Williams RJ, 1982 : Detection and partial characterization of two bovine pregnancy-specific proteins. Biol Reprod 26, 925 933.
Chagas e Silva J, Lopes da Costa L, Robalo Silva J, 2002 : Plasma progesterone profiles and factors affecting embryo-fetal mortality following embryo transfer in dairy cattle. Theriogenology 58, 51 59.
Chavatte-Palmer P, de Sousa N, Laigre P, Camous S, Ponter AA, Beckers JF, Heyman Y, 2006 : Ultrasound fetal measurements and pregnancy associated glycoprotein secretion in early pregnancy in cattle recipients carrying somatic clones. Theriogenology 66, 829 840.
Deshpande L, Singal SP, Georgie KC, Lohan IS, 1990 : Periovulatory endocrine profile in Murrah buffaloes under two superovulatory regimes. Theriogenology 33, 210.
Ectors FJ, Drion PV, Delval A, Smith LC, Sulon J, Zaaijer D, Szenci O, Remy B, Beckers JF, Ectors F, 1996 : Interests of pregnancy follow-up in cows after embryo transfer special focusing on IVP and NT origin. 12th Annual Meeting AETE, 13-14 September, Lyon, France, pp. 95 102.
El Amiri B, Remy B, Sousa NM, Joris B, Otthiers NG, Perényi Z, Banga-Mboko H, Beckers JF, 2003 : Isolation and partial characterization of three pregnancy-associated glycoproteins from the ewe placenta. Mol Reprod Dev 64, 199 206.
El Amiri B, Remy B, Sousa NM, Beckers JF, 2004 : Isolation and characterization of eight pregnancy-associated glycoproteins present at high levels in the ovine placenta between day 60 and day 100 of gestation. Reprod Nutr Dev 44, 169 181.
El-Battawy KA, 1993 : Some Studies on Postpartum Anestrum in Buffaloes. MVSc thesis, Cairo University, 130 p.
El-Battawy KA, 2005 : Measurements of faecal progesterone metabolites using EIA as an aid for pregnancy diagnosis in buffalo cows. Egypt. J Basic Appl Physiol 4, 233 240.
FAO, 2001 : The state of food insecurity in the world 2001. http://www.fao.org.
Garbayo JM, Remy B, Alabart JL, Folch J, Wattiez R, Falmagne P, Beckers JF, 1998. Isolation and partial characterization of a pregnancy-associated glycoprotein family from the goat placenta. Biol Reprod 58, 109 115.
Garbayo JM, Green JA, Manikkam M, Beckers JF, Kiesling DO, Ealy AD, Roberts RM, 2000 : Caprine pregnancy-associated glycoproteins (PAG): their cloning, expression, and evolutionary relationship to other PAG. Mol Reprod Dev 57, 311 322.
Gonzalez F, Sulon J, Garbayo JM, Batista M, Cabrera F, Calero PO, Gracia A, Beckers JF, 2000 : Secretory profiles of pregnancy-associated glycoproteins at different stages of pregnancy in the goat. Reprod Dom Anim 35, 79 82.
Green JA, Xie S, Quan X, Bao B, Gan X, Mathialagan N, Beckers JF, Roberts RM, 2000 : Pregnancy-associate bovine and ovine glycoproteins exhibit spatially and temporally distinct expression patterns during pregnancy. Biol Reprod 62, 1624 1631.
Greenwood FC, Hunter WM, Glover JS, 1963 : The preparation of 131-I labelled human growth hormone of high specific radioactivity. Biochem J 89, 114 123.
Heyman Y, Chavatte-Palmer P, LeBourhis D, Camous S, Vignon X, Renard JP, 2002 : Frequency and occurrence of late-gestation losses from cattle cloned embryos. Biol Reprod 66, 6 13.
Humblot P, Camous S, Martal J, Charlery J, Jeanguyot N, Thibier M, Sasser RG, 1988 : Pregnancy-specific protein B, progesterone concentrations and embryonic mortality during early pregnancy in dairy cows. J Reprod Fertil 83, 215 223.
Jainudeen MR, Bongso TA, Tan HS, 1983 : Postpartum ovarian activity and uterine involution in the suckled swamp buffalo (Bubalus bubalis). Reprod Dom Anim 5, 81 90.
Kamonpatana M, Chantaraprateep P, Ngramsuriyaroj C, 1981 : A hers test for non-pregnancy plasma progesterone levels in the selection of swamp for oestrus synchronisation. Br Vet J 137, 173 175.
Karen A, Beckers JF, Sulon J, de Sousa NM, Szabados K, Reczigel J, Szenci O, 2003 : Early diagnosis in sheep by progesterone and pregnancy-associated glycoprotein tests. Theriogenology 59, 1941 1948.
Ledezma-Torres RA, Beckers JF, Holtz W, 2006 : Assessment of plasma profile of pregnancy associated glycoprotein (PAG) in sheep with a heterologous (anti-caPAG(55 + 59)) RIA and its potential for diagnosing pregnancy. Theriogenology 66, 906 912.
Lopez-Gatius F, Garbayo JM, Santolaria P, Yaniz J, Ayad A, Sousa NM, Beckers JF, 2007 : Milk production correlates negatively with plasma levels of pregnancy-associated glycoprotein (PAG) during the early fetal period in high producing dairy cows with live fetuses. Dom Anim Endocrinol 32, 29 42.
Mialon MM, Camous S, Renand G, Martal J, Menissier F, 1993 : Peripheral concentrations of a 60 kDa pregnancy serum protein during gestation and after calving and in relationship to embryonic mortality in cattle. Reprod Nutr Dev 33, 269 282.
Oltenacu PA, Ferguson JD, Lendor AJ, 1990 : Economic evaluation of pregnancy diagnosis in dairy cattle. A decision analysis approach. J Dairy Sci 37, 2826 2831.
Patel OV, Sulon J, Beckers JF, Takahashi T, Hirako M, Sasaki N, Domeki I, 1997 : Plasma bovine pregnancy-associated glycoprotein concentrations throughout gestation in relationship to fetal number in the cow. Eur J Endocrinol 137, 423 428.
Perenyi Z, Szenci O, Sulon J, Drion PV, Beckers JF, 2002a : Comparison of the ability of three radioimmunoassay to detect pregnancy-associated glycoproteins in bovine plasma. Reprod Dom Anim 37, 100 104.
Perenyi Z, Szenci O, Drion PV, Banga-Mboko H, Sousa NM, El Amiri B, Beckers JF, 2002b : Aspartic proteinase members secreted by the ruminant placenta: specificity of three radioimmunoassay systems for the measurement of pregnancy-associated glycoproteins. Reprod Dom Anim 37, 324 329.
Perera BMAO, Pathiraja N, Abeywardena SA, Motha MXJ, Abeygunawardena H, 1981 : Early pregnancy diagnosis in buffaloes from plasma progesterone concentration. Vet Rec 106, 104 106.
Pieterse MC, Szenci O, Willemse AH, Bajcsy CSA, Dieleman SJ, Taverne MAM, 1990 : Early pregnancy diagnosis in cattle by means of linear-array real-time ultrasound scanning of the uterus and a qualitative and quantitative milk progesterone test. Theriogenology 33, 697 708.
Ranilla MJ, Sulon J, Carro MD, Mantecón AR, Beckers JF, 1994 : Plasmatic profiles of pregnancy associated glycoprotein and progesterone levels during gestation in churra and merino sheep. Theriogenology 42, 537 545.
Sasser RG, Ruder CA, Ivani KA, Butler JE, Hamilton WC, 1986 : Detection of pregnancy by radioimmunoassay of a novel pregnancy-specific protein in serum of cows and a profile of serum concentrations during gestation. Biol Reprod 35, 936 942.
Sharma YP, Kaker ML, 1990 : Monitoring ovarian cyclicity in postpartum Murrah buffalo through milk progesterone enzymeimmunoassay. Theriogenology 33, 915 923.
Sheehan KT, Casper RF, Yen SSC, 1982 : Luteal phase defects induced by an agonist of luteinizing hormone releasing factor: A model for fertility control. Science 215, 170.
Singh G, Singh GB, Sharma RD, Nanda AS, 1988 : Ovarian and uterine responses in relation to norgestomet-PMSG treatment in the true anoestrous buffalo. Anim Reprod Sci 16, 71 74.
Sousa NM, Garbayo JM, Figueiredo JR, Sulon J, Gonçalves PBD, Beckers JF, 1999 : Pregnancy-associated glycoprotein and progesterone profiles during pregnancy and post partum in native goats from the north-east of Brazil. Small Rum Res 32, 137 147.
Szenci O, Beckers JF, Humblot P, Sulon J, Sasser RG, Taverne MAM, 1998 : Comparison of ultrasonography, bovine pregnancy-specific protein B, and bovine pregnancy-associated glycoprotein 1 tests for pregnancy detection in dairy cows. Theriogenology 50, 77 88.
Szenci O, Beckers JF, Sulon J, Bevers MM, Börzsönyi L, Fodor L, Kovacs F, Taverne MAM, 2003 : Effect of induction of late embryonic morttality on plasma profile of pregnancy associated glycoprotein 1 in heifers. Vet J 165, 307 313.
Thirapatsukunk T, Entwistle KW, Gartner SJW, 1978 : Plasma progesterone levels as an early pregnancy test in beef cattle. Theriogenology 9, 323 332.
Xie S, Low BG, Nagel RJ, Kramer KK, Anthony RV, Zoli AP, Beckers JF, Roberts RM, 1991 : Identification of the major pregnancy specific antigens of cattle and sheep as inactive members of the aspartic proteinase family. Proc Natl Acad Sci USA 88, 10247 10251.
Zoli AP, Beckers JF, Wouters-Ballman P, Closset J, Falmagne P, Ectors F, 1991 : Purification and characterization of a bovine pregnancy-associated glycoprotein. Biol Reprod 45, 1 10.
Zoli AP, Demez P, Beckers JF, Reznik M, Beckers A, 1992a : Light and electron microscopic immunolocalization of bovine pregnancy associated glycoprotein in the bovine placentome. Biol Rreprod 46, 623 629.
Zoli AP, Guilbault LA, Delahaut P, Ortiz WB, Beckers JF, 1992b : Radioimmunoassay of a bovine pregnancy-associated glycoprotein in serum: its application for pregnancy diagnosis. Biol Reprod 46, 83 92.