[en] The aim of this experiment was to study the kinetics of anti-eCG (equine chorionic gonadotrophin) antibodies in relation to eCG dose (8 or 25 IU) and number of injections (n = 11) in comparison with a control group (no injection), and to relate antibody production to sexual receptivity and productivity of rabbit does. In all, 124 lactating primiparous rabbit does were inseminated every 35 days for a year. Just before eCG injection (48 h before insemination), blood samples were collected from all the does to assay anti-eCG antibodies. The anti-eCG antibody binding rate, regardless of the injected dose, shows that none of the does developed detectable anti-eCG antibodies before the 7th injection. The level of detectable anti-eCG antibodies began to show an increase at the 7th injection and was significant only for the 25 IU dose at the 11th injection. At the end of the experiment, 15% and 39% of does treated with 8 and 25 IU, respectively, developed immunity to eCG (binding rate >6%: higher binding rate of the control group). Consequently, the immune response depends on the eCG dose and on the number of injections. Moreover, productivity of does estimated from the number of weaned rabbits produced per insemination is not influenced by the level of eCG antibodies (7.0 and 6.9 for binding rate <6% and binding rate 6%, respectively). Only 19 inseminations (n = 6 and n = 13 for 8 and 25 IU, respectively) were made on hyperimmune does. Consequently, the immune response to eCG seems to be marginal for rabbit does. Moreover, under the described experimental conditions, reproductive performances of hyperimmune does were not affected
Drion, Pierre ; Université de Liège - ULiège > Services généraux (Faculté de médecine vétérinaire) > Méth. expér. des anim. de labo et éthique en expér. animale
Language :
English
Title :
Evolution of anti-eCG antibodies in response to eCG doses and number of injections. Relation with rabbit does productivity
Publication date :
2008
Journal title :
Animal
ISSN :
1751-7311
eISSN :
1751-732X
Publisher :
Cambridge University Press 2008., Cambridge, United Kingdom
Baril G, Remy B, Vallet JC and Beckers JF 1992. Effect of repeated use of progestagen-PMSG treatment for oestrus control in dairy goats out of breeding season. Reproduction in Domestic Animals 27, 161-168.
Bodin L, Brice G, Remy B, Maurel MC and Beckers JF 1995. Effects of repeated PMSG treatments on sheep reproduction. In Proceedings of the 46th Annual Meeting of the EAAP, 1995, Prague, Czech Republic.
Boiti C, Castellini C, Canali C, Zampini D and Monaci M 1995. Long term effect of PMSG on rabbit does reproductive performance. World Rabbit Science 3, 51-56.
Bourdillon A, Chmitelin F, Jarrin D, Parez V and Rouiller H 1992. Effect of PMSG treatment on breeding result of artificial inseminated rabbits. Journal of Applied Rabbit Research 15, 530-537.
Canali C, Boiti C, Castellini C and Zampini D 1991. Riposta anticorpale delle coniglie trattate ripetutamente con PMSG nella pratica della sincronizzazione degli estri. 2o Meeting Nazionale Studio della Efficienza Riproduttiva degli Animali di Interesse Zootecnico, Bergamo, Italie 24 Novembre, 1989, 103-108.
Clarke IJ 1973. Repeated gonadotrophic stimulation of ewes. Proceedings of New Zealand Society of Animal Production 33, 204.
Drion PV, Remy B, Houtain JY, Mc Namara M, Baril G, Heyman Y, Cognie Y, Theau-Clément M, Leboeuf B, Ectors F, Segers K and Beckers JF 1998. Utilisation répétée des gonadotropines exogènes dans le contrôle de la reproduction : justifications, relations structure-activité biologique, effets secondaires potentiels. Une synthèse. Annales de Médecine Vétérinaire 142, 373-396.
International Rabbit Reproduction Group 2005. Recommendations and guidelines for applied reproduction trials with rabbit does. World Rabbit Science 13, 147-164.
Lebas F, Theau-Clément M, Remy B, Drion P and Beckers JF 1996. Production of anti-PMSG antibodies and its relation to the productivity of rabbit does. World Rabbit Science 4, 57-62.
Rebollar PG, Milanès A, Pereda N, Millán P, Cano P, Esquifino AI, Villarroel M, Silván G and Lorenzo PL 2006. Oestrus synchronisation of rabbit does at early post-partum by doe-litter separation or ECG injection: reproductive parameters and endocrine profiles. Animal Reproduction Science 93, 218-230.
Roy F, Maurel MC, Combarnous Y, Briois JP, Pobel T and Deletang F 1995. Etude de la réponse immunitaire observée chez les ovins et les caprins traités avec PMSG dans le cadre de l'insémination artificielle. Rencontres Recherches Ruminants 3, 395-398.
Roy F, Maurel MC, Combes B, Vaiman D, Cribiu EP, Lantier I, Pobel T, Delétang F, Combarnous Y and Guillou F 1999. The negative effect of repeated equine chorionic gonadotropin treatment on subsequent fertility in Alpine goats is due to a humoral immune response involving the Major Histocompatibility Complex. Biology of reproduction 60, 805-813.
SAS (Statistical Analysis Systems Institute) 1993. Release 6.09 SAS Technical Report P-243.SAS/STAT® Software. Cary, NC, USA.
Stradaioli G, Castellini C, Monaci M, Canali C, Verini Supplizi A and Boiti C 1994. Effetti del PMSG sulle prestazioni riproduttive delle coniglie: risultati di un quadriennio di sperimentazione. 68 Meeting Nazionale Studio della Efficienza Riproduttiva degli Animali di Interesse Zootecnico, 29 Aprile, 1994, Bergamo, Italie, 153-157.
Theau-Clément M, Lebas F and Mercier P 2007. Influence of different eCG doses on sexual receptivity and productivity of rabbit does. World Rabbit Science, in press.
Thorell JI and Johansson BG 1971. Enzymatic iodination of polypeptides with 125I to high specific activity. Biochemica and Biophysica Acta 251, 363-369.