Reference : A homologous radioimmunoassay for quantification of insulin-like growth factor-bindin...
Scientific journals : Article
Life sciences : Agriculture & agronomy
Life sciences : Veterinary medicine & animal health
A homologous radioimmunoassay for quantification of insulin-like growth factor-binding protein-2 in blood from cattle.
Vleurick, Lieve mailto [> > > >]
Renaville, Robert mailto [Université de Liège - ULiège > > Gembloux Agro-Bio Tech >]
VandeHaar, M. [> > > >]
Hornick, Jean-Luc mailto [Université de Liège - ULiège > Département de productions animales > Nutrition des animaux domestiques >]
Istasse, Louis mailto [Université de Liège - ULiège > Département de productions animales > Nutrition des animaux domestiques >]
Parmentier, Isabelle mailto [Université de Liège - ULiège > > Centre interfacultaire de formation des enseignants (CIFEN) >]
Bertozzi, C. [> > > >]
Van Eenaeme, Christian [Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Portetelle, Daniel mailto [> >]
Journal of Dairy Science
American Dairy Science Association
Yes (verified by ORBi)
[en] Animals ; Blotting, Western ; Cattle/blood ; Circadian Rhythm ; Female ; Growth Hormone/pharmacology ; Insulin-Like Growth Factor Binding Protein 2/blood ; Pregnancy ; Radioimmunoassay/methods ; Reference Values ; Sensitivity and Specificity ; Sheep/blood ; Swine/blood
[en] Insulin-like growth factor-I and -II (IGF-I, IGF-II) circulate in biological fluids bound to six different IGF-binding proteins that regulate IGF bioactivity. The IGF-binding protein-2 is regulated by growth hormones, and its concentration depends on nutrition and physiological state. Specific antibodies directed against bovine IGF-binding protein-2 were produced, and IGF-binding protein-2 levels in bovine blood samples were quantified by radioimmunoassay. Parallel displacement curves showed strong cross-reactivity with bovine and ovine plasma, were low with porcine plasma, and no cross-reactivity with rat or chicken plasma. Addition of IGF-I or -II to a control pool of bovine plasma did not significantly alter control IGF-binding protein-2 values in a radioimmunoassay. Six nycthemeral periods, determined for three young bulls bled on two occasions, showed that IGF-binding protein-2 plasma levels were stable throughout the day; two or three samples were sufficient to characterize the animal. Cows treated with recombinant bovine somatotropin (bST) had significantly lower serum levels of IGF-binding protein-2 than did control cows. Furthermore, IGF-binding protein-2 levels were dramatically increased at the onset of lactation. This radioimmunoassay for bovine IGF-binding protein-2, which enables quantitative assessment of IGF-binding protein-2 concentration in cattle, confirmed that IGF-binding protein-2 concentrations are depressed by administration of bST, enhanced after calving, and showed absence of diurnal variation.
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