Reference : Penicillin-sensitive DD-carboxypeptidase from Streptomyces strain R 61
Scientific journals : Article
Life sciences : Microbiology
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/85160
Penicillin-sensitive DD-carboxypeptidase from Streptomyces strain R 61
English
Leyh-Bouille, Mélina [Université de Liège - ULiège > Institut de Botanique > Service de Microbiologie > > > >]
Coyette, Jacques [Université de Liège - ULiège > Institut de Botanique > Service de Microbiologie > > > >]
Ghuysen, Jean-Marie [Université de Liège - ULiège > Institut de Botanique > Service de Microbiologie > > > >]
Idczak, Josiane [> > > >]
Perkins, Harold R. [National Institute for Medical Research - London > > > >]
Nieto, Manuel [National Institute for Medical Research - London > > > >]
25-May-1971
Biochemistry
American Chemical Society
10
11
2163-2170
Yes (verified by ORBi)
International
0006-2960
1520-4995
Washington
DC
[en] alanine ; carboxypeptidases antagonists & inhibitors ; cephalothin ; chromatography deae-cellulose ; chromatographyl ; corynebacterium ; edetic acid ; electrophoresis ; hydrogen-ion concentration ; ion exchange resins ; kinetics ; magnesium ; penicillins ; peptides ; streptomyces enzymology ; gel
[en] The D-alanyl-D-alanine carboxypeptidase excreted by the penicillin-sensitive Streptomyces strain R 61 is an anionic protein at pH 6.5. It does not require cations for its action. By comparison with the penicillin-resistant DD-carboxypeptidase from Streptomyces albus G, the penicillin-sensitive R 61 enzyme has high Km values with all the peptides which were used but the alignment of catalytic groups is more effective in promoting hydrolysis of the peptides (high Vmax values). Moreover, the substrate structures required by the two enzymes show some differences. In contrast to the albus G DD -carboxypeptidase which is either not, or very little, affected by penicillins, the R 61 enzyme is inhibited by low dose levels of penicillin G and by cephalothin
Fonds de la Recherche Fondamentale Collective
Researchers ; Professionals
http://hdl.handle.net/2268/85160

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Open access
GHUYSEN_1971_penicillin-sensitive-dd-carboxypeptidase.pdfPublisher postprint767.44 kBView/Open

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.