Reference : Crystallographic data for a penicillin receptor : exocellular DD-carboxypeptidase-tra...
Scientific journals : Letter to the editor
Life sciences : Microbiology
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/85133
Crystallographic data for a penicillin receptor : exocellular DD-carboxypeptidase-transpeptidase from Streptomyces R61
English
Knox, James R. [University of Connecticut - UCONN > Biological Sciences Group and Institute of Materials Science > > >]
DeLucia, Mary L. [University of Connecticut - UCONN > Biological Sciences Group and Institute of Materials Science > > > > > >]
Murthy, N. S. [University of Connecticut - UCONN > Biological Sciences Group and Institute of Materials Science > > >]
Kelly, Judith A. [University of Connecticut - UCONN > Biological Sciences Group and Institute of Materials Science > > >]
Moews, Paul C. [University of Connecticut - UCONN > Biological Sciences Group and Institute of Materials Science > > >]
Frère, Jean-Marie mailto [Université de Liège - ULiège > Faculté de Médecine, Institut de Botanique > Service de Microbiologie > >]
Ghuysen, Jean-Marie [Université de Liège - ULiège > Faculté de Médecine, Institut de Botanique > Service de Microbiologie > > >]
15-Jan-1979
Journal of Molecular Biology
Academic Press
127
2
217-218
Yes (verified by ORBi)
International
0022-2836
1089-8638
London
United Kingdom
[en] carboxypeptidases ; molecular weight ; muramoylpentapeptide carboxypeptidase ; streptomyces/enzymology ; x-ray diffraction
[en] A pencillin-sensitive enzyme, the exocellular DD-carboxypeptidase-transpeptidase from Streptomyces R61, has been crystallized from polyethylene glycol (Mr = 6000 to 7500) solution at pH 7•6. X-ray examination of the orthorhombic crystals shows the space group is P212121, with unit cell dimensions a = 51•1 Å, B = 67•4 Å, and C = 102•9 Å. With four molecules of molecular weight 38,000, the Å3/dalton ratio for the cell is 2•33. The crystals are stable to irradiation for 75 hours and are suitable for structure analysis to at least 2•4 Å resolution. The radius of gyration of the molecule in solution at pH 6.8 is 20.8 Å.
United States Public Health Service
Researchers ; Professionals
http://hdl.handle.net/2268/85133
10.1016/0022-2836(79)90241-9

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