[en] Twenty-three colonic adenocarcinomas were studied by immunofluorescence with antisera against components of the basement membrane (type IV collagen, laminin, and heparan sulfate-rich proteoglycan) as well as antisera against antigens of the connective tissue (type III collagen, fibronectin, and hyaluronectin). Marked alterations of the basement membranes were consistently observed on staining with each one of the first three antisera. In contrast, staining of the normal components of connective tissue was in most cases as intense in tumors as in normal colonic mucosa. Hyaluronectin, a marker of peritumoral stroma, was found to be present in 13 out of 16 tumors studied. In six metastatic lymph nodes, tumor foci were sometimes surrounded by antigens of the basement membrane. But these antigens were never found in the cytoplasm of cancer cells.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Burtin, P.
Chavanel, G.
Foidart, Jean-Michel ; Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique - Labo de biologie des tumeurs et du développement
Language :
English
Title :
Immunofluorescence study of the antigens of the basement membrane and the peritumoral stroma in human colonic adenocarcinomas.
Publication date :
1983
Journal title :
Annals of the New York Academy of Sciences
ISSN :
0077-8923
eISSN :
1749-6632
Publisher :
New York Academy of Sciences, New York, United States - New York
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