Reference : Structural investigations on cell walls of Nocardia sp. The wall lipid and peptidogly...
Scientific journals : Article
Life sciences : Microbiology
Life sciences : Biochemistry, biophysics & molecular biology
Structural investigations on cell walls of Nocardia sp. The wall lipid and peptidoglycan moieties of Nocardia kirovani
Vacheron, Marie-Jeanne [Université Claude Bernard - Lyon 1 - UCLB > > Laboratoire de Chimie Biologique > >]
Guinand, Micheline [Université Claude Bernard - Lyon 1 - UCLB > > Laboratoire de Chimie Biologique > >]
Michel, Georges [Université Claude Bernard - Lyon 1 - UCLB > > Laboratoire de Chimie Biologique > >]
Ghuysen, Jean-Marie [Université de Liège - ULiège > > Service de Microbiologie > > >]
European Journal of Biochemistry
Blackwell Science
Yes (verified by ORBi)
United Kingdom
[en] amino acid sequence ; carotenoids ; cell wall analysis ; chemical phenomena ; chemistry ; chromatography gas ; chromatography gel ; disaccharides ; electrophoresis paper ; esters ; fatty acids ; fatty acids unsaturated ; ketones ; lipids analysis ; mass spectrometry ; nocardia analysis ; peptides ; peptidoglycan analysis ; polysaccharides bacterial analysis ; spectrophotometry infrared ; triglycerides ; cytology
[en] The walls of Nocardia kirovani are composed of three main constituents: the peptidoglycan matrix, a polysaccharide polymer and a variety of free and bound lipids. The free lipids represent 17.5% (dry weight) of the walls and consist for the major part of C16–18 fatty acids and nocardic acids, and for the minor part, of nocardones, triglycerides and carotenoid pigments. The nocardic acids were identified as tri- and tetra-unsaturated, α-branched, β-hydroxylated compounds C58H110O3—C66H124O3 the nocardones as tri-and tetra-unsaturated ketones C57H106O—C63H1180O, and the main carotenoid pigment as phlei-xantophylle palmitate. Esters of glycerol with C14, C16, C18 fatty acids and, for some of them, with odd numbered poly-unsaturated acids containing 35 to 45 carbon atoms, were also identified. Bound lipids represent about 20% (dry weight) of the walls and consist mainly of nocardic acids probably ester-linked to an arabinogalactan polymer. The peptidoglycan (about 40% dry weight) is composed of β-1,4-N-acetylglucosaminyl-N-glycolylmuramic acid disaccharide units that are substituted by diamidated L-Ala-D-αGln-(L)-A2pm-(D)-NH2 tripeptides and diamidated l-Ala-d-αGln-(L)-A2pm-(D)-NH2-(L)-d-Ala tetrapeptides, where A2pm is meso-diaminopimelic acid. Crosslinking between some of the peptide units is mediated through D-Ala-(D)-A2pm linkages (peptidoglycan of chemotype I).
Fonds de la Recherche Fondamentale Collective
Researchers ; Professionals

File(s) associated to this reference

Fulltext file(s):

Open access
VACHERON_1972_structural.pdfPublisher postprint2.07 MBView/Open

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.