Article (Scientific journals)
Solubilization and isolation of the membrane-bound DD-carboxypeptidase of Streptococcus faecalis ATCC9790. Properties of the purified enzyme
Coyette, Jacques; Ghuysen, Jean-Marie; Fontana, Roberta
1978In European Journal of Biochemistry, 88 (1), p. 297-305
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Keywords :
carboxypeptidases/*metabolism; carrier proteins/isolation & purification; cell membrane/*enzymology; cloxacillin/metabolism; enterococcus faecalis/drug effects/*enzymology; muramoylpentapeptide carboxypeptidase/isolation & purification/*metabolism; oligopeptides/metabolism; penicillin g/metabolism; penicillins/metabolism; solubility
Abstract :
[en] Streptococcus faecalis ATCC 9790 possesses six membrane-bound, penicillin-binding proteins. That numbered 6 (Mr 43000) is the most abundant one and is the DD-carboxypeptidase studied previously. The enzyme has been solubilized and purified to the stage where one single protein band can be detected by gel electrophoresis. The purification procedure does not alter the properties that the enzyme exhibits when it is membrane-bound. The DD-carboxypeptidase itself may be a killing target for penicillin in S. faecalis.
Disciplines :
Microbiology
Biochemistry, biophysics & molecular biology
Author, co-author :
Coyette, Jacques;  Université de Liège - ULiège > Faculté de Médecine, Institut de Botanique > Service de Microbiologie
Ghuysen, Jean-Marie ;  Université de Liège - ULiège > Faculté de Médecine, Institut de Botanique > Service de Microbiologie
Fontana, Roberta;  Universita di Sassari > Istituto di Microbiologia
Language :
English
Title :
Solubilization and isolation of the membrane-bound DD-carboxypeptidase of Streptococcus faecalis ATCC9790. Properties of the purified enzyme
Publication date :
17 July 1978
Journal title :
European Journal of Biochemistry
ISSN :
0014-2956
eISSN :
1432-1033
Publisher :
Blackwell Science, Oxford, United Kingdom
Volume :
88
Issue :
1
Pages :
297-305
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
NIH - National Institutes of Health [US-MD]
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since 27 January 2011

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