Solubilization and isolation of the membrane-bound DD-carboxypeptidase of Streptococcus faecalis ATCC9790. Properties of the purified enzyme

Coyette, Jacques; Ghuysen, Jean-Marie; Fontana, Roberta

doi:10.1111/j.1432-1033.1978.tb12450.x

Article (Scientific journals)

Solubilization and isolation of the membrane-bound DD-carboxypeptidase of Streptococcus faecalis ATCC9790. Properties of the purified enzyme

Coyette, Jacques; Ghuysen, Jean-Marie; Fontana, Roberta

1978 • In European Journal of Biochemistry, 88 (1), p. 297-305

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/83043

DOI
10.1111/j.1432-1033.1978.tb12450.x

PubMed
97082

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Keywords :

carboxypeptidases/*metabolism; carrier proteins/isolation & purification; cell membrane/*enzymology; cloxacillin/metabolism; enterococcus faecalis/drug effects/*enzymology; muramoylpentapeptide carboxypeptidase/isolation & purification/*metabolism; oligopeptides/metabolism; penicillin g/metabolism; penicillins/metabolism; solubility

Abstract :

[en] Streptococcus faecalis ATCC 9790 possesses six membrane-bound, penicillin-binding proteins. That numbered 6 (Mr 43000) is the most abundant one and is the DD-carboxypeptidase studied previously. The enzyme has been solubilized and purified to the stage where one single protein band can be detected by gel electrophoresis. The purification procedure does not alter the properties that the enzyme exhibits when it is membrane-bound. The DD-carboxypeptidase itself may be a killing target for penicillin in S. faecalis.

Disciplines :

Microbiology
Biochemistry, biophysics & molecular biology

Author, co-author :

Coyette, Jacques; Université de Liège - ULiège > Faculté de Médecine, Institut de Botanique > Service de Microbiologie

Ghuysen, Jean-Marie ; Université de Liège - ULiège > Faculté de Médecine, Institut de Botanique > Service de Microbiologie

Fontana, Roberta; Universita di Sassari > Istituto di Microbiologia

Language :

English

Title :

Solubilization and isolation of the membrane-bound DD-carboxypeptidase of Streptococcus faecalis ATCC9790. Properties of the purified enzyme

Publication date :

17 July 1978

Journal title :

European Journal of Biochemistry

ISSN :

0014-2956

eISSN :

1432-1033

Publisher :

Blackwell Science, Oxford, United Kingdom

Volume :

Issue :

Pages :

297-305

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

F.R.S.-FNRS - Fonds de la Recherche Scientifique
NIH - National Institutes of Health

Available on ORBi :

since 27 January 2011

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Bibliography

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