Reference : β-Lactamase expression in Streptomyces cacaoi
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/81172
β-Lactamase expression in Streptomyces cacaoi
English
Urabe, Hiroaki [Meiji College of Chemistry (Tokyo) > Department of Biochemistry > > >]
Lenzini, Mauro V [Université de Liège - ULiège > Institut de Chimie > Service de Microbiologie > >]
Mukaide, Masakazu [Meiji College of Chemistry (Tokyo) > Department of Biochemistry > > >]
Dusart, Jean [Université de Liège - ULiège > Institut de Chimie > Service de Microbiologie > >]
Nakano, Michiko M [Meiji College of Chemistry (Tokyo) > Department of Biochemistry > > >]
Ghuysen, Jean-Marie [Université de Liège - ULiège > Institut de Chimie > Service de Microbiologie > >]
Ogawara, Hiroshi [Meiji College of Chemistry (Tokyo) > Department of Biochemistry > > >]
1-Nov-1990
Journal of Bacteriology
American Society for Microbiology (ASM)
172
11
6427-6434
Yes (verified by ORBi)
International
0021-9193
1098-5530
Washington
DC
[en] amino acid sequence ; ampicillin resistance/genetics ; base sequence ; escherichia coli/genetics ; gene expression regulation, bacterial ; genes, bacterial ; molecular sequence data ; plasmids ; promoter regions, genetic ; restriction mapping ; streptomyces/enzymology/*genetics ; beta-Lactamases/*genetics
[en] Plasmids were prepared by inserting genomic DNA fragments from Streptomyces cacaoi within the mel gene of plasmid pIJ702. The inserted DNA fragments contain the beta-lactamase-encoding bla gene and upstream nucleotide sequences of various lengths. The transcription start point of bla was identified by nuclease S1 mapping. Upstream nucleotide sequences of sufficient lengths had an enhancing effect on beta-lactamase production by the Streptomyces host. The dot blot hybridization assay revealed that this effect was exerted at the transcriptional level. Experimental evidence strongly suggests that the underlying mechanism involves, at least in part, one or several trans-acting elements. In one of the constructs, in which the upstream nucleotide sequence was reduced to 0.3 kb, the bla promoter was present but the bla gene was expressed by readthrough from a promoter, possibly the mel promoter, of the pIJ702 vector.
Researchers ; Professionals
http://hdl.handle.net/2268/81172

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