[en] The penicillin-resistant Enterococcus hirae R40 has a typical profile of membrane-bound penicillin-binding proteins (PBPs) except that the 71 kDa PBP5 of low penicillin affinity represents about 50% of all the PBPs present. Water-soluble tryptic-digest peptides were selectively produced from PBP5, their N-terminal regions were sequenced and synthetic oligonucleotides were used as primers to generate a 476 bp DNA fragment by polymerase chain reaction. On the basis of these data, the PBP5-encoding gene was cloned in Escherichia coli by using pBR322 as vector. The gene, included in a 7.1 kb insert, had the information for a 678-amino acid-residue protein. PBP5 shows similarity, in the primary structure, with the high-molecular-mass PBPs of class B. In particular, amino acid alignment of the enterococcal PBP5 and the methicillin-resistant staphylococcal PBP2' generates scores that are 30, for the N-terminal domains, and 53, for the C-terminal domains, standard deviations above that expected for a run of 20 randomized pairs of proteins having the same amino acid compositions as the two proteins under consideration.
Research Center/Unit :
CIP - Centre d'Ingénierie des Protéines - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
el Kharroubi, Aboubaker; Université de Liège - ULiège > Institut de Chimie > Centre d'Ingénierie des Protéines
Jacques, Philippe ; Université de Liège - ULiège > Institut de Chimie > Centre d'Ingénierie des Protéines
Piras, Graziella; Université de Liège - ULiège > Institut de Chimie > Centre d'Ingénierie des Protéines
Van Beeumen, Jozef; Rijksuniversiteit-Gent > Laboratorium voor Microbiologie en Microbiële Genetica
Coyette, Jacques; Rijksuniversiteit-Gent > Laboratorium voor Microbiologie en Microbiele Genetica
Ghuysen, Jean-Marie ; Université de Liège - ULiège > Institut de Chimie > Centre d'Ingénierie des Protéines
Language :
English
Title :
The Enterococcus Hirae R40 Penicillin-Binding Protein 5 and the Methicillin-Resistant Staphylococcus Aureus Penicillin-Binding Protein 2' Are Similar
Publication date :
01 December 1991
Journal title :
Biochemical Journal
ISSN :
0264-6021
eISSN :
1470-8728
Publisher :
Portland Press, London, United Kingdom
Volume :
280
Issue :
Pt 2
Pages :
463-469
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE] FRSM - Fonds de la Recherche Scientifique Médicale [BE]