Article (Scientific journals)
Presence of human antibodies reacting with Candida albicans O-linked oligomannosides revealed by using an enzyme-linked immunosorbent assay and neoglycolipids
Hayette, Marie-Pierre; Strecker, Gerald; Faille, Christine et al.
1992In Journal of Clinical Microbiology, 30 (2), p. 411-417
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Keywords :
Candida albicans; neoglycolipids; ELISA
Abstract :
[en] In order to study the presence of antibodies directed against Candida albicans O-linked oligomannosides (oligomannosides O) in patient sera, we have developed an enzyme-linked immunosorbent assay (ELISA) involving neoglycolipids constructed with these residues (NGLO). Oligomannosides O released by mild alkaline degradation of the C. albicans cell wall phosphopeptidomannan (PPM) contained one to seven mannose residues, among which the quantitatively major components, mannobiose and mannotriose, were shown by 1H nuclear magnetic resonance to contain exclusively alpha (1-2) linkages. The pool of oligomannosides was converted to neoglycolipids by coupling them to 4-hexadecylaniline in an equimolar reaction checked by thin-layer chromatography. We have tested against these neoantigens, coated on ELISA plates, 15 pairs of sera corresponding to individual seroconversions observed in 15 patients during the course of a mycological and serological survey of candidiasis. For all patients, seroconversions resulted in an increased level of antibodies against NGLO. A significant correlation was observed between the results of ELISA-NGLO, ELISA involving the original PPM molecule, and routine antibody detection tests, indirect immunofluorescence assay, and cocounterimmunoelectrophoresis. These results therefore demonstrate the synthesis of human antibodies reactive with oligomannosides O constitutive of the C. albicans mannan molecule which have been previously described as exhibiting an inhibitory effect on human lymphocytic proliferation.
Disciplines :
Laboratory medicine & medical technology
Author, co-author :
Hayette, Marie-Pierre ;  Université de Liège - ULiège > Microbiologie médicale
Strecker, Gerald
Faille, Christine
Dive, Daniel
Camus, Daniel
Mackenzie, D. W.
Poulain, Daniel
Language :
English
Title :
Presence of human antibodies reacting with Candida albicans O-linked oligomannosides revealed by using an enzyme-linked immunosorbent assay and neoglycolipids
Publication date :
February 1992
Journal title :
Journal of Clinical Microbiology
ISSN :
0095-1137
eISSN :
1098-660X
Publisher :
American Society for Microbiology (ASM), Washington, United States - District of Columbia
Volume :
30
Issue :
2
Pages :
411-417
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 30 December 2010

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