Reference : Development of a mass spectrometry method for the determination of a melanoma biomark...
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
http://hdl.handle.net/2268/8029
Development of a mass spectrometry method for the determination of a melanoma biomarker, 5-S-cysteinyldopa, in human plasma using solid phase extraction for sample clean-up.
English
Martin, Gaëlle mailto [Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments >]
Chiap, Patrice [Centre Hospitalier Universitaire de Liège - CHU > > Pharmacologie clinique >]
Paquet, Philippe mailto [Centre Hospitalier Universitaire de Liège - CHU > > Dermatopathologie >]
Pierard, Gérald mailto [Centre Hospitalier Universitaire de Liège - CHU > > Dermatopathologie >]
De Tullio, Pascal mailto [Université de Liège - ULiège > Département de pharmacie > Chimie pharmaceutique >]
Martin, Yves [> > > >]
Rozet, Eric mailto [Université de Liège - ULiège > Département de pharmacie > Chimie analytique >]
Hubert, Philippe mailto [Université de Liège - ULiège > Département de pharmacie > Chimie analytique >]
Crommen, Jacques mailto [Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments >]
Fillet, Marianne mailto [Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments >]
2007
Journal of Chromatography. A
Elsevier Science
1156
1-2
141-8
Yes (verified by ORBi)
International
0021-9673
1873-3778
Amsterdam
The Netherlands
[en] Chromatography, High Pressure Liquid/methods ; Cysteinyldopa/blood ; Humans ; Mass Spectrometry/methods ; Melanoma/blood ; Reproducibility of Results ; Solid Phase Extraction/methods ; Tumor Markers, Biological/blood
[en] 5-S-cysteinyldopa is a well-known pigment intermediate and analysis of its plasma concentration is interesting for the early diagnosis, as well as for evaluation of treatment and follow-up of malignant melanoma. A determination method of 5-SCD in human plasma was developed using solid phase extraction (SPE) on disposable cartridges and liquid chromatography electrospray mass spectrometry (LC-ESI-MS-MS). Compound's sensitivity to light and oxidation requires the addition of anti-oxidative agents (AO), to work in acidic media at 4 degrees C and to avoid light exposure of samples since blood collection. Different solid phases involving covalent binding to phenylboronic groups or dual retention mechanisms were evaluated and extraction cartridges containing both hydrophobic and strong cation exchange functionalities were finally selected. The LC separation of 5-SCD from endogenous catecholamines was achieved by gradient elution on a C18 stationary phase. 5-SCD was detected by multiple reaction monitoring (MRM) performed on ES(+) generated ions. Finally, the method was prevalidated in the lower ng/ml range. Good results with respect to accuracy, trueness and precision were obtained.
http://hdl.handle.net/2268/8029
10.1016/j.chroma.2006.12.088

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