Reference : Radioimmunoassay of bovine placental lactogen using recombinant and native preparatio...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/7983
Radioimmunoassay of bovine placental lactogen using recombinant and native preparations: determination of fetal concentrations across gestation
English
Alvarez-Oxiley, A. V. [> > > >]
Melo de Sousa, Noelita mailto [Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction >]
Hornick, Jean-Luc mailto [Université de Liège - ULiège > Département de productions animales > Nutrition des animaux domestiques >]
Touati, Kamal mailto [Université de Liège - ULiège > Département clinique des animaux de production (DCP) > Département clinique des animaux de production (DCP) >]
van der Weijden, G. C. [> > > >]
Taverne, M. A. M. [> > > >]
Szenci, O. [> > > >]
Sulon, Joseph [Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction >]
Debliquy, P. [> > > >]
Beckers, Jean-François mailto [Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction >]
2007
Reproduction, Fertility and Development
Csiro Publishing
19
7
877-885
Yes (verified by ORBi)
International
1031-3613
1448-5990
Collingwood
[en] fetal serum ; glycosylated form
[en] Concentrations of bovine placental lactogen ( bPL) were determined in fetal plasma samples by twelve double-antibody competitive radioimmunoassay systems ( RIA I - XII) based on either recombinant bPL ( non- glycosylated) or native bPL ( glycosylated). Both preparations were used as standard and tracer, and for primary antisera production. The minimum detection limit measured by these RIA varied from 0.02 to 0.6 ng bPLmL(-1). The coefficients of correlation of different bPL RIA systems were up to 90% ( P< 0.0001) when each RIA was tested against the average values of all twelve RIA systems. All developed RIA were used to investigate the incidence of different bPL isoforms in bovine fetal serum samples ( n= 71). Fetal concentrations ranged from 11.8 to 35.7 ng mL(-1) at the third month and from 1.1 to 13.5 ngmL(-1) at the ninth month of gestation. They tended to decrease with advancing gestation. In general, those RIA systems that used recombinant bPL as the standard measured higher values than those using the native bPL preparation. These differences decreased toward the end of gestation ( P< 0.05), suggesting a lower rate of glycosylation. Our results provide evidence of different glycosylated isoforms of bPL in fetal serum at different gestation periods.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/7983
10.1071/RD06173

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