Comparison of five radioimmunoassay systems for PAG measurement: Ability to detect early pregnancy in cows

Ayad, A.; Melo de Sousa, Noelita; Sulon, Joseph; Iguer-Ouada, M.; Beckers, Jean-François

doi:10.1111/j.1439-0531.2006.00804.x

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Article (Scientific journals)

Comparison of five radioimmunoassay systems for PAG measurement: Ability to detect early pregnancy in cows

Ayad, A.; Melo de Sousa, Noelita; Sulon, Joseph et al.

2007 • In Reproduction in Domestic Animals, 42 (4), p. 433-440

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https://hdl.handle.net/2268/7980

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10.1111/j.1439-0531.2006.00804.x

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17635783

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Keywords :

radioimmunoassay; pregnancy; cows

Abstract :

[en] This study was conducted to describe the minimum detection limit, reproducibility, accuracy, specificity and parallelism of different pregnancy-associated glycoprotein radioimmunoassay (PAG-RIA) systems: RIA-497, RIA-706, RIA-780, RIA-809 and RIA-Pool. Their ability to distinguish between non-pregnant and pregnant females at day 30 after artificial insemination (Al) was investigated. The antisera were raised in rabbits against different PAG preparations. All RIA systems proved to be sensitive, repeatable and accurate for measuring PAG concentrations. The dilutions of plasma samples taken at an early stage of pregnancy were found to be parallel to the standard curves. No cross-reaction was observed with different carbohydrates, either with Pregnant Marc Serum Gonadotropin (PMSG) or human Chorionic Gonadotropin (hCG). The concentrations of PAG in pregnant females at day 30 after Al were shown to be higher with the use of antisera R#706, R#780, R#809 and Pool when compared with antiserum R#497. All the RIA systems gave 100% sensitivity and negative predictive values. On the other hand, the use of antisera R#780 and R#809 resulted in lower specificity and positive predictive values. The present study clearly shows that the ability of PAG-RIA systems to diagnose pregnancy specifically at day 30 after Al can be improved by using a combination of antisera raised against different forms of PAG.

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Ayad, A.; Université de Liège - ULiège > Faculty of veterinary medicine

Melo de Sousa, Noelita ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction

Sulon, Joseph ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction

Iguer-Ouada, M.; University Abderahmane Mira, Bejaia, Algeria > Organism and Populations Biology, Faculty of Life and Nature Sciences

Beckers, Jean-François ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction

Language :

English

Title :

Comparison of five radioimmunoassay systems for PAG measurement: Ability to detect early pregnancy in cows

Publication date :

2007

Journal title :

Reproduction in Domestic Animals

ISSN :

0936-6768

eISSN :

1439-0531

Publisher :

Blackwell Publishing, Berlin, Germany

Volume :

Issue :

Pages :

433-440

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 07 March 2009

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