Western analyses of pregnancy-associated glycoprotein family (PAG) in placental extracts of various mammals

Bella, Amina; Melo de Sousa, Noelita; Dehimi, M. L.; Watts, J.; Beckers, Jean-François

doi:10.1016/j.theriogenology.2007.08.002

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Article (Scientific journals)

Western analyses of pregnancy-associated glycoprotein family (PAG) in placental extracts of various mammals

Bella, Amina; Melo de Sousa, Noelita; Dehimi, M. L. et al.

2007 • In Theriogenology, 68 (7), p. 1055-1066

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https://hdl.handle.net/2268/7976

DOI
10.1016/j.theriogenology.2007.08.002

PubMed
17850858

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Keywords :

pregnancy-associated glycoprotein; immunoblotting; ammonium sulfate precipitation; placenta; yolk sac

Abstract :

[en] The present study was conducted in order to analyze the inummoreactivity, of placental extracts of several animal species and humans against the following three groups of PAG antisera: anti-boPAG-1 (R#497), -boPAG-II (R#435), and -caPAG (R#706). Placental proteins were obtained after extraction at neutral pH, followed by ammonium sulfate (A.S.) precipitation, dialysis, and lyophilization. The immunoreactivity of different placental extracts was revealed by the use of monodimensional SDS-PAGE, followed by blotting on nitrocellulose membrane and the identification of immunoreactive proteins after incubation with PAG antisera (Western blot technique). A strong immunoreactivity of proteins from synepitheliochorial placenta (cattle, sheep, goat, bison, buffalo, and deer) was demonstrated in both 20-50% and 50-80% A.S. fractions using the three antisera. Proteins from species with epitheliochorial placenta presented variable profiles of detected PAG-like proteins: in the sow, many inummoreactive forms were revealed by antisera boPAG-I and boPAG-II, whereas in the dromedary, only two forms were revealed by anti-boPAG-II. Concerning other species, our protocols showed for the first time a cross-reaction between PAG antisera with proteins extracted from dog, alpaca, dromedary, sea lion, and human placenta. (C) 2007 Elsevier Inc. All rights reserved.

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Bella, Amina ; Université de Liège - ULiège > Doct. sc. vété. (Bologne)

Melo de Sousa, Noelita ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction

Dehimi, M. L.

Watts, J.

Beckers, Jean-François ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction

Language :

English

Title :

Western analyses of pregnancy-associated glycoprotein family (PAG) in placental extracts of various mammals

Publication date :

2007

Journal title :

Theriogenology

ISSN :

0093-691X

eISSN :

1879-3231

Publisher :

Elsevier Science Inc, New York, United States - New York

Volume :

Issue :

Pages :

1055-1066

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 07 March 2009

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