[en] Whole-cell protein electrophoretic analysis of the type strain of Streptococcus difficile (LMG 15799) revealed
that this organism was indistinguishable from Streptococcus agalactiae strains. Although LMG 15799'r (T =
type strain) was originally described as serologically untypeable, we found that this strain was a group B
streptococcus belonging to the capsular polysaccharide antigen type Ib group. The biochemical reactivity of S.difficile, which differed from the biochemical reactivity of typical S. agalactiae strains mainly by being less
versatile, is similar to the biochemical reactivity of other group B, type Ib streptococci isolated from poikilothermic animals, such as fish and frogs.
Disciplines :
Microbiology
Author, co-author :
Vandamme, Peter; University of Ghent - RUG > Faculty of Sciences > Laboratory of Microbiology
Devriese, L. A.; University of Ghent - RUG > Faculty of Veterinary Medicine
Pot, B.; University of Ghent - RUG > BCCM/LMG Culture Collection
Kersters, K.; University of Ghent - RUG > Faculty of Sciences > Laboratory of Microbiology
Bentley, R. W., J. A. Leigh, and M. D. Collins. 1991. Intrageneric structure of Streptococcus based on comparative analysis of small-subunit rRNA sequences. Int. J. Syst. Bacteriol. 41:487-494.
Brenner, D. J., G. R. Fanning, A. V. Rake, and K. E. Johnson. 1969. Batch procedure for the thermal elution of DNA from hydroxyapatite. Anal. Biochem. 28:447-459.
Christie, R., N. E. Atkins, and E. Munch-Petersen. 1944. A note on a lytic phenomenon shown by group B streptococci. Aust. J. Exp. Biol. Med. Sci. 22:193-197.
Collins, M. D., B. A. Phillips, and P. Zanoni. 1989. Deoxyribonucleic acid homology studies of Lactobacillus casei, Lactobacillus paracasei sp. nov., subsp. paracasei and subsp. tolerans, and Lactobacillus rhamnosus sp. nov., comb. nov. Int. J. Syst. Bacteriol. 39:105-108.
Collins, M. D., J. A. E. Farrow, V. Katie, and O. Kandler. 1984. Taxonomic studies on streptococci of serological groups E, P, U and V: description of Streptococcus porcinus sp. nov. Syst. Appl. Microbiol. 5:402-413.
Costas, M. 1992. Classification, identification, and typing of bacteria by the analysis of their one-dimensional polyacrylamide gel electrophoretic protein patterns, p. 351-408. In A. Chambrach, M. J. Dunn, and B. J. Radola (ed.), Advances in electrophoresis, vol. 5. VCH Verlagsgesellschaft, Weinheim, Germany.
Devriese, L. A. 1991. Streptococcal ecovars associated with different animal species: epidemiological significance of serogroups and biotypes. J. Appl. Bacteriol. 71:478-483.
Eldar, A., Y. Bejerano, and H. Bercovier. 1994. Streptococcus shiloi and Streptococcus difficile: two new Streptococcal species causing a meningoencephalitis in fish. Curr. Microbiol. 28:139-143.
Eldar, A., P. F. Frelier, L. Assenta, P. W. Varner, S. Lawhon, and H. Bercovier. 1995. Streptococcus shiloi, the name for an agent causing septicemie infection in fish, is a junior synonym of Streptococcus iniae. Int. J. Syst. Bacteriol. 45:840-842.
Elliot, J. A., R. R. Facklam, and C. B. Richter. 1990. Whole-cell protein patterns of nonhemolytic group B, type Ib streptococci isolated from humans, mice, cattle, frogs, and fish. J. Clin. Microbiol. 28:628-630.
Ezaki, T., R. Facklam, N. Takeuchi, and E. Yabuuchi. 1986. Genetic relatedness between the type strain of Streptococcus anginosus and minute-colony-forming beta-hemolytic streptococci carrying different Lancefield group antigens. Int. J. Syst. Bacteriol. 36:345-347.
Farrow, J. A. E., and M. D. Collins. 1984. Taxonomic studies on streptococci of serological groups C, G and L and possibly related taxa. Syst. Appl. Microbiol. 5:483-493.
Hertel, C., W. Ludwig, B. Pot, K. Kersters, and K.-H. Schleifer. 1993. Differentiation of lactobacilli occurring in fermented milk products by using oligonucleotide probes and electrophoretic protein profiles. Syst. Appl. Microbiol. 14:463-467.
Heyndrickx, M., K. Vandemeulebroecke, B. Hoste, P. Janssen, K. Kersters, P. De Vos, N. A. Logan, N. Ali, and R. C. W. Berkeley. 1996. Reclassification of Paenibacillus (formerly Bacillus) pulvifaciens (Nakamura 1984) Ash et al. 1994, a later subjective synonym of Paenibacillus (formerly Bacillus) larvae (White 1906) Ash et al. 1994, as a subspecies of P. larvae, with emended descriptions of P. larvae as P. larvae subsp. larvae and P. larvae subsp. pulvifaciens. Int. J. Syst. Bacteriol. 46:270-279.
Heyndrickx, M., K. Vandemeulebroecke, P. Scheldeman, B. Hoste, K. Kersters, P. De Vos, N. A. Logan, A. M. Aziz, N. Ali, and R. C. W. Berkeley. 1995. Paenibacillus (formerly Bacillus) gordonae (Pichinoty et al. 1986) Ash et al. 1994 is a later subjective synonym of Paenibacillus (formerly Bacillus) validus (Nakamura 1984) Ash et al. 1994: emended description of P. validus. Int. J. Syst. Bacteriol. 45:661-669.
Kersters, K., B. Pot, D. Dewettinck, U. Torck, M. Vancanneyt, L. Vauterin, and P. Vandamme. 1994. Identification and typing of bacteria by protein electrophoresis, p. 51-66. In F. G. Priest, A. Ramos-Cormenzana, and B. Tyndall (ed.), Bacterial diversity and systematics. Plenum Press, New York, N.Y.
Kilpper-Bälz, R., G. Fischer, and K. H. Schleifer. 1982. Nucleic acid hybridization of group N and group D streptococci. Curr. Microbiol. 7:245-250.
Lancefield, R. C. 1933. A serological differentiation of human and other groups of hemolytic streptococci. J. Exp. Med. 59:571-591.
Owen, R. J., and P. J. H. Jackman. 1982. The similarities between Pseudomonas paucimobilis and allied bacteria derived from analysis of deoxyribonucleic acids and electrophoretic protein patterns. J. Gen. Microbiol. 128: 2945-2954.
Pier, G. B., and S. H. Madin. 1976. Streptococcus iniae sp. nov., a beta-hemolytic streptococcus isolated from Amazon freshwater dolphin, Inia geoffrensis. Int. J. Syst. Bacteriol. 26:545-553.
Pot, B., C. Hertel, W. Ludwig, P. Descheemaeker, K. Kersters, and K. H. Schleifer. 1993. Identification and classification of Lactobacillus acidophilus, L. gasseri, and L. johnsonii strains by SDS-PAGE and rRNA-targeted oligonucleotide probe hybridisations. J. Gen. Microbiol. 139:513-517.
Pot, B., P. Vandamme, and K. Kersters. 1994. Analysis of electrophoretic whole-organism protein fingerprints, p. 493-521. In M. Goodfellow and A. G. O'Donnell (ed.), Modern microbial methods. Chemical methods in prokaryotic systematics. J. Wiley and Sons, Ltd., Chichester, United Kingdom.
Rotta, J. 1986. Pyogenic hemolytic streptococci, p. 1047-1054. In P. H. A. Sneath, N. S. Mair, M. E. Sharpe, and J. G. Holt (ed.), Bergey's manual of systematic bacteriology, vol. 2. The Williams & Wilkins Co., Baltimore, Md.
Schleifer, K.-H., M. Ehrmann, C. Beimfohr, E. Brockmann, W. Ludwig, and R. Amann. 1995. Application of molecular methods for the classification and identification of lactic acid bacteria. Int. Dairy J. 5:1081-1094.
Skaar, I., P. Gaustad, T. Tonjum, B. Holm, and H. Stenwig. 1994. Streptococcus phocae sp. nov., a species isolated from clinical specimens from seals. Int. J. Syst. Bacteriol. 44:646-650.
Teixeira, L. M., R. R. Facklam, A. G. Steigerwalt, N. E. Pigott, V. L. Merquior, and D. J. Brenner. 1995. Correlation between phenotypic characteristics and DNA relatedness within Enterococcus faecium strains. J. Clin. Microbiol. 33:1520-1523.
Vandamme, P., J. Hommez, M. Vancanneyt, M. Monsieurs, B. Hoste, B. T. Cookson, C. H. Wirsing von König, K. Kersters, and P. J. Blackall. 1995. Bordetella hinzii sp. nov., isolated from poultry and humans. Int. J. Syst. Bacteriol. 45:37-45.
Vandamme, P., B. Pot, E. Falsen, K. Kersters, and J. De Ley. 1990. Intra-and interspecific relationships of veterinary campylobacters revealed by numerical analysis of electrophoretic protein profiles and DNA:DNA hybridizations. Syst. Appl. Microbiol. 13:295-303.
Vandamme, P., B. Pot, E. Falsen, K. Kersters, and L. A. Devriese. 1996. Taxonomic study of Lancefield streptococcal groups C, G, and L (Streptococcus dysgalactiae) and proposal of S. dysgalactiae subsp. equisimilis subsp. nov. Int. J. Syst. Bacteriol. 46:774-781.
Vandamme, P., M. Vancanneyt, B. Pot, L. Mels, B. Hoste, D. Dewettinck, L. Vlaes, C. Van Den Borre, R. Higgins, J. Hommez, K. Kersters, J.-P. Butzler, and H. Goossens. 1992. Polyphasic taxonomic study of the emended genus Arcobacter with Arcobacter butzleri comb. nov. and Arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated from veterinary specimens. Int. J. Syst. Bacteriol. 42:344-356.
Wanger, A. R., and G. M. Dunny. 1985. Development of a system for genetic and molecular analysis of Streptococcus agalactiae. Res. Vet. Sci. 38:202-208.
Wayne, L. G., D. J. Brenner, R. R. Colwell, P. A. D. Grimont, P. Kandler, M. I. Krichevsky, L. H. Moore, W. E. C. Moore, R. G. E. Murray, E. Stackebrandt, M. P. Starr, and H. G. Trüper. 1987. Report of the Ad Hoc Committee on Reconciliation of Approaches to Bacterial Systematics. Int. J. Syst. Bacteriol. 37:463-464.
Whiley, R. A., and J. M. Hardie. 1989. DNA-DNA hybridization studies and phenotypic characteristics of strains within the 'Streptococcus milleri' group. J. Gen. Microbiol. 135:2623-2633.
Wilkinson, H. W., L. G. Thacker, and R. R. Facklam. 1973. Nonhemolytic group B streptococci of human, bovine, and ichthyic origin. Infect. Immun. 7:496-498.
