[en] In bronchoalveolar lavage (BAL) fluid from ventilated patients, cytotoxic oxidant activity is correlated with neutrophil activation. The aim of the present study was to investigate the hypothesis that BAL fluid induces activation of the transcription nuclear factor-kappaB (NF-kappaB) in human alveolar cells, in correlation with inflammatory mediators. We measured endotoxin, inflammatory cytokines [Interleukin-1beta (IL-1beta), IL-8], nitrated proteins and the activity of myeloperoxidase (MPO) in BAL fluid from ventilated patients developing bronchopneumonia (n = 19 samples) or with acute respiratory distress syndrome (ARDS) (n = 14), and from ARDS/infection-free patients (n = 11). We also exposed alveolar cells to the BAL fluid or to human MPO, H2O2 or HOCl, and tested nuclear extracts for the activation of NF-kappaB. IL-1beta, IL-8, nitrated protein, MPO and endotoxin levels were significantly higher in BAL fluid from patients with bronchopneumonia than in that from the ARDS and ARDS/infection-free groups. A correlation was observed between IL-8 and MPO values (r = 0.82). The level of NF-kappaB activity induced by the BAL fluid was correlated with levels of IL-1beta (P < 0.001), IL-8 (P < 0.005) and MPO (P < 0.002), and with the neutrophil count (P < 0.002), and was higher for BAL fluid from the bronchopneumonia group. NF-kappaB activation by MPO was also demonstrated. The activation of NF-kappaB by BAL fluid, especially that from bronchopneumonia patients, suggests that a similar phenomenon may occur in vivo, leading to potential amplification of the inflammatory reaction.
Disciplines :
Laboratory medicine & medical technology
Author, co-author :
Nys, Monique ; Centre Hospitalier Universitaire de Liège - CHU > Soins intensifs
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
Bibliography
Bernard, G. R., Artigas, A., Brigham, K. L. et al. (1994) The American-European consensus conference on ARDS: Definitions, mechanisms, relevant outcomes, and clinical trial coordination. Am. J. Respir. Crit. Care Med. 149, 818-824
Kolleff, M. H. and Schuster, D. P. (1995) The acute respiratory distress syndrome. N. Engl. J. Med. 332, 27-37
Donnelly, S. C., Strieter, R. M., Kunkel, S. L. et al. (1993) Interleukin-8 and the development of adult respiratory distress syndrome in at-risk patient groups. Lancet 341, 643-647
Jacobs, R. F., Tabor, D. R., Burks, A. W. and Campbell, D. G. (1989) Elevated interleukin-1 release by human alveolar macrophages during the adult respiratory distress syndrome. Am. Rev. Respir. Dis. 140, 1686-1692
Suter, P. M., Suter, S., Girardin, E., Roux-Lombard, P., Grau, G. E. and Dayer, J. M. (1992) High bronchoalveolar levels of tumor necrosis factor and its inhibitors, interleukin-1, interferon, and elastase, in patients with adult respiratory distress syndrome after trauma, shock, or sepsis. Am. Rev. Respir. Dis. 145, 1016-1022
Meduri, G. U., Kohler, G., Headley, S., Tolley, E., Stentz, F. and Postlethwaite, A. (1995) Inflammatory cytokines in BAL of patients with ARDS: Persistent elevation over time predicts poor outcome. Chest 108, 1303-1314
Millar, A. B., Singer, M., Meager, A., Foley, N. M., Johnson, N. M. and Rook, G. A. W. (1989) Tumor necrosis factor in bronchopulmonary secretions of patients with adult respiratory distress syndrome. Lancet ii, 712-714
Cochrane, C. G. (1991) Cellular injury, by oxidants. Am. J. Med. 91 (Suppl. 3C), 23S-30S
Haddad, I. Y., Pataki, G., Hu, P., Galliani, C., Beckman, J. S. and Matalon, S. (1994) Quantification of nitrotyrosine levels in lung sections of patients and animals with acute lung injury. J. Clin. Invest. 94, 2407-2413
Kooy, N. W., Royall, J. A., Ye, Y. Z., Kelly, D. R. and Beckman, J. S. (1995) Evidence for in vivo peroxynitrite production in human acute lung injury. Am. J. Respir. Crit. Care Med. 15, 1250-1254
Mathy-Hartert, M., Damas, P., Nys, M. et al. (2000) Nitrated proteins in bronchoalveolar lavage fluid of patients at risk of ventilator-associated bronchopneumonia. Eur. Respir. J. 16, 296-301
Abraham, E. (2000) NF-κB activation. Crit. Care Med. 28 (Suppl.), N100-N104
Christman, J. W., Sadikot, R. T. and Blackwell, T. S. (2000) The role of nuclear factor-κB in pulmonary diseases. Chest 117, 1482-1487
Barnes, P. J. and Karim, M. (1997) Nuclear factor-κB: A pivotal transcription factor in chronic inflammatory diseases. N. Engl. J. Med. 336, 1066-1071
Schwartz, M. D., Moore, E. E., Moore, F. A. et al. (1996) Nuclear factor-κB is activated in alveolar macrophages from patients with acute respiratory distress syndrome. Crit. Care Med. 24, 1285-1292
Moine, P., McIntyre, R., Schwartz, M. D. et al. (2000) NF-κB regulatory mechanisms in alveolar macrophages from patients with acute respiratory distress syndrome. Shock 13, 85-91
Böhrer, H., Qiu, F., Zimmermann, T. et al. (1997) Role of NF-κB in the mortality of sepsis. J. Clin. Invest. 100, 972-985
Baeuerle, P. A. and Henkel, T. (1994) Function and activation of NF-κB in the immune system. Annu. Rev. Immunol. 12, 141-179
Arenzana-Seisdedos, F., Turpin, P., Rodriguez, M. et al. (1997) Nuclear localization of I kappa B alpha promotes active transport of NF-kappa B from the nucleus to the cytoplasm. J. Cell Sci. 110, 369-378
Meyer, M., Schreck, R. and Baeuerle, P. A. (1993) H2O2 and antioxidants have opposite effects on activation of NF-κB and AP-1 in intact cells: AP-1 as secondary antioxidant-responsive factor. EMBO J. 12, 2005-2015
Schmidt, K. N., Amstad, P., Cerutti, P. and Baeuerle, P. A. (1995) The roles of hydrogen peroxide and superoxide as messengers in the activation of transcription factor NF-κB. Chem. Biol. 2, 13-22
Beg, A. A., Finco, T. S., Nanternet, P. V. and Baldwin, A. S. (1993) Tumor necrosis factor and interleukin-1 lead to phosphorylation and loss of IκBα: A mechanism for NF-κB activation. Mol. Cell. Biol. 13, 3301-3310
Meduri, G. U. and Chastre, J. (1992) The standardization of bronchoscope techniques for ventilator-associated pneumonia. Chest 102, 557S-564S
Nys, M., Ledoux, D., Canivet, J.-L., DeMol, P., Lamy, M. and Damas, P. (2000) Correlation between endotoxin level and bacterial count in bronchoalveolar lavage fluid of ventilated patients. Crit. Care Med. 28, 2825-2830
Matthay, M. A. and Wiener-Kronish, J. P. (1990) Intact epithelial barrier function is critical for the resolution of alveolar edema in humans. Am. Rev. Respir. Dis. 142, 1250-1257
Mathy-Hartert, M., Deby-Dupont, G., Melin, P., Lamy, M. and Deby, C. (1996) Bactericidal activity against Pseudomonas aeruginosa is acquired by cultured human monocyte-derived macrophages after uptake of myeloperoxidase. Experientia 52, 167-174
Schoonbroodt, S., Legrand-Poels, S., Best-Belpomme, M. and Piette, J. (1997) Activation of the NF-κB transcription factor in a T-lymphocytic cell line by hypochlorous acid. Biochem. J. 321, 777-785
Schoonbroodt, S., Ferreira, V., Best-Belpomme, M. et al. (2000) Crucial role of the amino-terminal tyrosine residue 42 and the carboxyl-terminal PEST domain of IκBα in NF-κB activation by an oxidative stress. J. Immunol. 164, 4292-4300
Van der Vliet, A., Eiserich, J. P., Shigenaga, M. K. and Cross, C. E. (1999) Reactive nitrogen species and tyrosine nitration in the respiratory tract. Epiphenomena or a pathophysiological mechanism of disease? Am. J. Respir. Crit. Care Med. 160, 1-9
Eiserich, J. P., Hristova, M., Cross, C. E. et al. (1998) Formation of nitric oxide-derived inflammatory, oxidants by myeloperoxidase in neutrophils. Nature (London) 391, 393-397
Lin, C. H., Kuan, I. H., Lee, H. M. et al. (2001) Induction of cyclooxygenase-2 protein by lipoteichoic acid from Staphylococcus aureus in human pulmonary epithelial cells: Involvement of a nuclear factor-kappa B-dependent pathway. Br. J. Pharmacol. 134, 543-552
Miller, E. J., Cohen, A. B. and Matthay, M. A. (1996) Increased interleukin-8 concentrations in the pulmonary edema fluid of patients with acute respiratory distress syndrome from sepsis. Crit. Care Med. 24, 1448-1454
Aggarwal, A., Baker, C. S., Evans, T. W. and Haslam, P. L. (2000) G-CSF and IL-8 but not GM-CSF correlate with severity of pulmonary neutrophilia in acute respiratory distress syndrome. Eur. Respir. J. 15, 895-901
Abul, H., Abul, A., Khan, I., Mathew, T. C., Ayed, A. and Al-Athary, E. (2001) Levels of IL-8 and myeloperoxidase in the lungs of pneumonia patients. Mol. Cell. Biochem. 217, 107-112
Weiland, J. E., Davis, W. B., Holter, J. F., Mohammed, J. R., Dorinsky, P. M. and Gadek, J. E. (1986) Lung neutrophils in the adult respiratory distress syndrome. Am. Rev. Respir. Dis. 133, 218-225
Pittet, J. F., Mackersie, R. C., Martin, T. R. and Matthay, M. A. (1997) Biological markers of acute lung injury: Prognostic and pathogenetic significance. Am. J. Respir. Crit. Care Med. 155, 1187-1205
Wewers, M. D., Herzyk, D. J. and Gadek, J. E. (1988) Alveolar fluid neutrophil elastase activity in the adult respiratory distress syndrome is complexed to alpha-2macroglobulin. J. Clin. Invest. 82, 1260-1267
Matthay, M. A. (1999) Conference summary: Acute lung injury. Chest 116, 119S-126S
Sittipunt, C., Steinberg, K. P., Ruzinski, J. T. et al. (2001) Nitric oxide and nitrotyrosine in the lungs of patients with acute respiratory distress syndrome. Am. J. Respir. Crit. Care Med. 163, 503-510
Parsey, M. V., Kaneko, D., Shenkar, R. and Abraham, E. (1999) Neutrophil apoptosis in the lung after hemorrhage or endotoxemia: Apoptosis and migration are independent of IL-1β. Clin. Immunol. 91, 219-225
Matute-Bello, G., Liles, W. C., Radella, F. et al. (1997) Neutrophil apoptosis in the acute respiratory distress syndrome. Am. J. Respir. Crit. Care Med. 156, 1969-1977
True, A. L., Rahman, A. and Malik, A. B. (2000) Activation of NF-κB induced by H2O2 and TNFα and its effects on ICAM-1 expression in endothelial cells. Am. J. Physiol. Lung Cell. Mol. Physiol. 279, L302-L311
Pugin, J., Ricou, B., Steinberg, K. P., Suter, P. M. and Martin, T. R. (1996) Proinflammatory activity in bronchoalveolar lavage fluids from patients with ARDS: A prominent role for interleukin-1. Am. J. Respir. Crit. Care Med. 153, 1850-1856
This website uses cookies to improve user experience. Read more
Save & Close
Accept all
Decline all
Show detailsHide details
Cookie declaration
About cookies
Strictly necessary
Performance
Strictly necessary cookies allow core website functionality such as user login and account management. The website cannot be used properly without strictly necessary cookies.
This cookie is used by Cookie-Script.com service to remember visitor cookie consent preferences. It is necessary for Cookie-Script.com cookie banner to work properly.
Performance cookies are used to see how visitors use the website, eg. analytics cookies. Those cookies cannot be used to directly identify a certain visitor.
Used to store the attribution information, the referrer initially used to visit the website
Cookies are small text files that are placed on your computer by websites that you visit. Websites use cookies to help users navigate efficiently and perform certain functions. Cookies that are required for the website to operate properly are allowed to be set without your permission. All other cookies need to be approved before they can be set in the browser.
You can change your consent to cookie usage at any time on our Privacy Policy page.
