BVD; DNA immunisation; E2; neutralisation; synthetic gene
Résumé :
[en] We report DNA immunisation experiments in cattle using plasmid constructs that encoded glycoprotein E2 from bovine viral diarrhoea virus (BVDV)-1 (E2.1) and BVDV-2 (E2.2). The coding sequences were optimised for efficient expression in mammalian cells. A modified leader peptide sequence from protein gD of BoHV1 was inserted upstream of the E2 coding sequences for efficient membrane export of the proteins. Recombinant E2 were efficiently expressed in COS7 cells and they presented the native viral epitopes as judged by differential recognition by antisera from cattle infected with BVDV-1 or BVDV-2. Inoculation of pooled plasmid DNA in young cattle elicited antibodies capable of neutralising viral strains representing the major circulating BVDV genotypes.
Dehan, Pierre ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Elouahabi, A.
Vandenbranden, M.
Ruysschaert, J. M.
Hamers, Claude ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Immunologie et vaccinologie
Pastoret, Paul-Pierre ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Département des maladies infectieuses et parasitaires
Kerkhofs, P.
Langue du document :
Anglais
Titre :
Sequence-optimised E2 constructs from BVDV-1b and BVDV-2 for DNA immunisation in cattle
Baker J.C., The clinical manifestations of bovine viral diarrhea infection, Vet. Clin. North Am. Food Anim. Pract. (1995) 11:425-445.
Barouch D., Rational design of gene-based vaccines, J. Pathol. (2006) 208:283-289. (Pubitemid 43126120)
Baxi M.K., Deregt D., Robertson J., Babiuk L.A., Schlapp T., Tikoo S.K., Recombinant bovine adenovirus type 3 expressing bovine viral diarrhea virus glycoprotein E2 induces an immune response in cotton rats, Virology (2000) 278:234-243. (Pubitemid 32002611)
Becher P., Orlich M., Thiel H.J., Complete genomic sequence of border disease virus, a pestivirus from sheep, J. Virol. (1998) 72:5165-5173. (Pubitemid 28215698)
Bevilacqua A., Ceriani M.C., Capaccioli S., Nicolin A., Post-transcriptional regulation of gene expression by degradation of messenger RNAs, J. Cell. Physiol. (2003) 195:356-372. (Pubitemid 36529864)
Bolin S.R., Ridpath J.F., Glycoprotein E2 of bovine viral diarrhea virus expressed in insect cells provides limited protection from systemic infection and disease, Arch. Virol. (1996) 141:1463-1477. (Pubitemid 26351178)
Boxus M., Tignon M., Roels S., Toussaint J.F., Walravens K., Benoit M.A., Coppe P., Letesson J.J., Letellier C., Kerkhofs P., DNA immunization with plasmids encoding fusion and nucleocapsid proteins of BRSV induces a strong cell-mediated immunity and protects calves against challenge, J. Virol. (2007) 81:6879-6889. (Pubitemid 46986624)
Boulanger D., Waxweiler S., Karelle L., Loncar M., Mignon B., Dubuisson J., Thiry E., Pastoret P.P., Characterization of monoclonal antibodies to bovine viral diarrhoea virus: evidence of a neutralizing activity against gp48 in the presence of goat antimouse immunoglobulin serum, J. Gen. Virol. (1991) 72:1195-1198.
Brunak S., Engelbrecht J., Knudsen S., Prediction of human mRNA donor and acceptor sites from the DNA sequence, J. Mol. Biol. (1991) 220:49-65. (Pubitemid 121004542)
Bruschke C.J., Moormann R.J., van Oirschot J.T., van Rijn P.A., A subunit vaccine based on glycoprotein E2 of bovine virus diarrhea virus induces fetal protection in sheep against homologous challenge, Vaccine (1997) 15:1940-1945. (Pubitemid 27504691)
Bruschke C.J., van Oirschot J.T., van Rijn P.A., An experimental multivalent bovine virus diarrhea virus E2 subunit vaccine and two experimental conventionally inactivated vaccines induce partial fetal protection in sheep, Vaccine (1999) 17:1983-1991. (Pubitemid 29172369)
Collen T., Morrison W.I., CD4(+) T-cell responses to bovine viral diarrhoea virus in cattle, Virus Res. (2000) 67:67-80. (Pubitemid 30204635)
Collen T., Carr V., Parsons K., Charleston B., Morrison W.I., Analysis of the repertoire of cattle CD4(+) T cells reactive with bovine viral diarrhoea virus, Vet. Immunol. Immunopathol. (2002) 87:235-238. (Pubitemid 34665243)
Couvreur B., Letellier C., Collard A., Quenon P., Dehan P., Hamers C., Pastoret P.P., Kerkhofs P., Genetic and antigenic variability in bovine viral diarrhea virus (BVDV) isolates from Belgium, Virus Res. (2002) 85:17-28. (Pubitemid 34310521)
De Moerlooze L., Lecomte C., BrownShimmer S., Schmetz D., Guiot C., Vandenbergh D., Allaer D., Rossius M., Chappuis G., Dina D., et al., Nucleotide sequence of the bovine viral diarrhoea virus Osloss strain: comparison with related viruses and identification of specific DNA probes in the 5' untranslated region, J. Gen. Virol. (1993) 74:1433-1438. (Pubitemid 23230264)
Elahi S.M., Shen S.H., Talbot B.G., Massie B., Harpin S., Elazhari Y., Recombinant adenoviruses expressing the E2 protein of bovine viral diarrhea virus induce humoral and cellular immune responses, FEMS Microbiol. Lett. (1999) 177:159-166. (Pubitemid 29327178)
Elouahabi A., Thiry M., Pector V., Ruysschaert J.M., Vandenbranden M., Calorimetry of cationic liposome-DNA complex and intracellular visualization of the complexes, Methods Enzymol. (2003) 373:313-332. (Pubitemid 41103291)
Gallei A., Orlich M., Thiel H.-J., Becher P., Noncytopathogenic pestivirus strains generated by nonhomologous recombination: alterations in the NS4A/NS4B coding region, J. Virol. (2005) 79:14261-14270. (Pubitemid 41552724)
Gregory D., Tascon R.E., Lowrie D.B., Repeated use of Qiagen columns in largescale preparation of plasmid DNA, in: Lowrie D.B., Whalen R.G. (Eds.), Methods in molecular medicine, vol. 29, DNA vaccines: methods and protocols, Humana Press Inc., Totowa, NJ, 2000.
Grigera P.R., Marzocca M.P., Capozzo A.V., Buonocore L., Donis R.O., Rose J.K., Presence of bovine viral diarrhea virus (BVDV) E2 glycoprotein in VSV recombinant particles and induction of neutralizing BVDV antibodies in mice, Virus Res. (2000) 69:3-15.
Haas J., Park E.C., Seed B., Codon usage limitation in the expression of HIV-1 envelope glycoprotein, Curr. Biol. (1996) 6:315-324.
Hamers C., Couvreur B., Dehan P., Letellier C., Lewalle P., Pastoret P.-P., Kerkhofs P., Differences in experimental virulence of bovine viral diarrhoea viral strains isolated from haemorrhagic syndromes, Vet. J. (2000) 160:250-258.
Hamers C., Couvreur B., Dehan P., Letellier C., Fischer L., Brun A.J., Lewalle P., Michaux C., Kerkhofs P., Pastoret P.-P., Assessment of clinical and virological protection afforded by an inactivated BVDV-1 commercial vaccine against a BVDV-2 challenge, Vet. Rec. (2003) 153:236-240. (Pubitemid 37062978)
Harada T., Tautz N., Thiel H.J., E2-p7 region of the bovine viral diarrhea virus polyprotein: processing and functional studies, J. Virol. (2000) 74:9498-9506.
Harpin S., Talbot B., Mbikay M., Elazhari Y., Immune response to vaccination DNA encoding the bovine viral diarrhoea virus major glycoprotein gp53 (E2), FEMS Microbiol. Lett. (1997) 146:229-234. (Pubitemid 27057727)
Harpin S., Hurley D., Mbikay M., Talbot B., Elazhari Y., Vaccination of cattle with a DNA plasmid encoding the bovine viral diarrhoea virus major glycoprotein E2, J. Gen. Virol. (1999) 80:3137-3144.
Houe H., Epidemiological features and economical importance of bovine viral diarrhoea virus (BVDV) infections, Vet. Microbiol. (1999) 64:89-107. (Pubitemid 29073588)
Howard C.J., Clarke M.C., Sopp P., Brownlie J., Immunity to bovine virus diarrhoea virus in calves: the role of different T-cell subpopulations analysed by specific depletion in vivo with monoclonal antibodies, Vet. Immunol. Immunopathol. (1992) 32:303-314.
Howard C.J., Clarke M.C., Brownlie J., Protection against respiratory infection with bovine virus diarrhoea virus by passively acquired antibody, Vet. Microbiol. (1989) 19:195-203.
Jones L.R., Weber L., Homologous recombination in bovine pestiviruses: Phylogenetic and statistic evidence, Infect. Genet. Evol. (2004) 4:335-343. (Pubitemid 39232984)
Kelling C.L., Evolution of bovine viral diarrhea virus vaccines, Vet. Clin. North Am. Food Anim. Pract. (2004) 20:115-129. (Pubitemid 38610129)
Kozak M., The scanning model for translation: an update, J. Cell Biol. (1989) 108:229-241. (Pubitemid 19065830)
Köhl W., Zimmer G., Greiser-Wilke I., Haas L., Moennig V., Herrler G., The surface glycoprotein E2 of bovine viral diarrhoea virus contains an intracellular localization signal, J. Gen. Virol. (2004) 85:1101-1111. (Pubitemid 38659774)
Köhl W., Grone A., Moennig V., Herrler G., Expression of the surface glycoprotein E2 of Bovine viral diarrhea virus by recombinant vesicular stomatitis virus, J. Gen. Virol. (2007) 88:157-165. (Pubitemid 46085126)
Kramps J.A., Magdalena J., Quak J., Weerdmeester K., Kaashoek M.J., Maris-Veldhuis M.A., Rijsewijk F.A., Keil G., van Oirschot J.T., A simple, specific, and highly sensitive blocking enzyme-linked immunosorbent assay for detection of antibodies to bovine herpesvirus 1, J. Clin. Microbiol. (1994) 32:2175-2181. (Pubitemid 24258460)
Krogh A., Larsson B., von Heijne G., Sonnhammer E.L.L., Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes, J. Mol. Biol. (2001) 305:567-580. (Pubitemid 33032862)
Kummerer B.M., Tautz N., Becher P., Thiel H., Meyers G., The genetic basis for cytopathogenicity of pestiviruses, Vet. Microbiol. (2000) 77:117-128.
Kweon C.H., Yoon Y.D., An S.H., Lee Y.S., Expression of envelope protein (E2) of bovine viral diarrhea virus in insect cells, J. Vet. Med. Sci. (1997) 59:415-419.
Lee Y.M., Tscherne D.M., Yun S.I., Frolov I., Rice C.M., Dual mechanisms of pestiviral superinfection exclusion at entry and RNA replication, J. Virol. (2005) 79:3231-3242. (Pubitemid 40330934)
Letellier C., Kerkhofs P., Wellemans G., Vanopdenbosch E., Detection and genotyping of bovine diarrhea virus by reverse transcription-polymerase chain amplification of the 5' untranslated region, Vet. Microbiol. (1999) 64:155-167. (Pubitemid 29073593)
Liang R., Van den Hurk J.V., Zheng C., Yu H., Pontarollo R.A., Babiuk L.A., Van den Hurk S., Immunization with plasmid DNA encoding a truncated, secreted form of the bovine viral diarrhea virus E2 protein elicits strong humoral and cellular immune response, Vaccine (2005) 23:5252-5262. (Pubitemid 41443431)
Liu M.A., DNA vaccines: a review, J. Intern. Med. (2003) 253:402-410. (Pubitemid 36418924)
Nielsen H., Engelbrecht J., Brunak S., von Heijne G., Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites, Protein Eng. (1997) 10:1-6.
Nobiron I., Thompson I., Browlie J., Collins M.E., Co-administration of Il-2 enhances antigen-specific immune response with DNA encoding the glycoprotein E2 of bovine diarrhoea virus, Vet. Microbiol. (2000) 76:129-142. (Pubitemid 30628533)
Nobiron I., Thompson I., Browlie J., Collins M.E., Cytokine adjuvancy of BVDV DNA vaccine enhances both humoral and cellular immune response in mice, Vaccine (2001) 19:4226-4235. (Pubitemid 32703644)
Nobiron I., Thompson I., Browlie J., Collins M.E., DNA vaccination against bovine diarrhoea virus induces humoral and cellular responses in cattle with evidence for protection against viral challenge, Vaccine (2003) 21:2082-2092.
Reinman I., Meyers G., Beer M., Transcomplementation of autonomously replicating Bovine viral diarrhea virus replicons with deletion in the E2 coding region, Virology (2003) 307:213-227. (Pubitemid 36344157)
Ridpath J.F., Bolin S.R., The genomic sequence of a virulent bovine viral diarrhea virus (BVDV) from the type 2 genotype: detection of a large genomic insertion in a noncytopathic BVDV, Virology (1995) 212:39-46.
Ridpath J.F., Neill J.D., Frey M., Landgraf J.G., Phylogenetic, antigenic and clinical characterization of type 2 BVDV from North America, Vet. Microbiol. (2000) 77:145-155.
Rümenapf T., Unger G., Strauss J.H., Thiel H.J., Processing of the envelope glycoproteins of pestiviruses, J. Virol. (1993) 67:3288-3294. (Pubitemid 23143701)
Schmitt J., Becher P., Thiel H.J., Keil G.M., Expression of bovine viral diarrhoea virus glycoprotein E2 by bovine herpesvirus-1 from a synthetic ORF and incorporation of E2 into recombinant virions, J. Gen. Virol. (1999) 80:2839-2848. (Pubitemid 29504655)
Tajima M., The prevalent genotypes of bovine viral diarrhea virus in Japan, Germany and the United States of America, Jpn J. Vet. Res. (2006) 54:129-134. (Pubitemid 46061460)
Toussaint J.F., Coen L., Letellier C., Dispas M., Gillet L., Vanderplasschen A., Kerkhofs P., Genetic immunisation of cattle against bovine herpesvirus 1: glycoprotein gD confers higher protection than glycoprotein gC or tegument protein VP8, Vet. Res. (2005) 36:529-544. (Pubitemid 40960230)
Toussaint J.F., Letellier C., Paquet D., Dispas M., Kerkhofs P., Prime-boost strategies combining DNA and inactivated vaccines confer high immunity and protection in cattle against bovine herpesvirus-1, Vaccine (2005) 23:5073-5081. (Pubitemid 41318155)
Van Regenmortel M.H.V., Fauquet C.M., Bishop E.B., Carstens E.B., Estes M.K., Lemon S.M., Maniloff J., Mayo M.A., McGeoch D.J., Pringle C.R., Wickner R.B., Virus taxonomy: classification and nomenclature of viruses, Seventh report of the International Committee on taxonomy of viruses, Academic Press, San Diego, 2000.
Vilcek S., Durkovic B., Kolesarova M., Paton D.J., Genetic diversity of BVDV: consequences for classification and molecular epidemiology, Prev. Vet. Med. (2005) 72:31-35. (Pubitemid 41627318)
Wang L., Sunyer J.O., Bello L.J., Fusion to C3d enhances the immunogenicity of the E2 glycoprotein of type 2 bovine diarrhea virus, J. Virol. (2004) 78:1616-1622. (Pubitemid 38176694)
Wang S., Farfan-Arribas D.J., Shen S., Chou T.H., Hirsch A., He F., Lu S., Relative contributions of codon usage, promoter efficiency and leader sequence to the antigen expression and immunogenicity of HIV-1 Env DNA vaccine, Vaccine (2006) 24:4531-4540. (Pubitemid 43776774)
