Effects of exercise and oral antioxidant supplementation enriched in (n-3) fatty acids on blood oxidant markers and erythrocyte membrane fluidity in horses
De Moffarts, Brieuc; Portier, Karine; Kirschvink, Nathalieet al.
[en] The aim of this study was to investigate in a placebo-controlled field study the effect of a (n - 3)-vitamin supplementation on erythrocyte membrane fluidity (ENIF), oxidant/antioxidant markers and plasmatic omega 3/omega 6 fatty acid ratio (FAR) in 12 eventing horses. Venous blood was sampled at rest before (PRE) and after (POST) a three week treatment period with either the supplement (group S, n = 6) or a placebo (group P, n = 6) as well as after 15 min (POST E15') and 24 h (POST E24h) after a standardised exercise test. The following markers were analysed: EMF, plasma antioxidant capacity of water and lipid soluble components, ascorbic acid, uric acid (UA), glutathione (reduced: GSH, oxidised: GSSG), vitamin E (Vit E), beta-carotene, glutathione peroxidase (GPx) and superoxide dismutase (SOD) activity, selenium, copper (Cu), zinc (Zn), oxidised proteins (Protox), lipid peroxides (Pool) and FAR. EMF did not differ between group S and P after treatment, but GPx remained unchanged in group S whereas it decreased in group P and plasma Cu/Zn ratio remained unchanged whereas it increased in group P. FAR were significantly increased in group S. Exercise induced a significant decrease of EMF (POST vs. E24h) in both groups, but which was significantly lower at E15' in group S than in group P. Exercise induced a significant increase of UA and ACW (POST vs. E15') and Protox (POST vs. E24h) in both groups. An exercise-related decrease in GSH and Pool (POST vs. E15') was found in group P, whereas Vit E and FAR (POST vs. E24h) significantly decreased in both groups. The study showed that exercise induced a decrease in ENIF in horses associated with changes of blood oxidative balance. The (omega-3)vitamin supplementation tested improved the oxidative balance poorly but delayed the exercise-induced decrease of EMF and increased the FAR.
Pincemail, Joël ; Centre Hospitalier Universitaire de Liège - CHU > Probiox
Art, Tatiana ; Université de Liège - ULiège > Département de sciences fonctionnelles > Phys. neuro-muscul., de l'effort - Méd. sport. des animaux
Lekeux, Pierre ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie
Language :
English
Title :
Effects of exercise and oral antioxidant supplementation enriched in (n-3) fatty acids on blood oxidant markers and erythrocyte membrane fluidity in horses
Publication date :
July 2007
Journal title :
Veterinary Journal
ISSN :
1090-0233
eISSN :
1532-2971
Publisher :
Elsevier Sci Ltd, Oxford, United Kingdom
Volume :
174
Issue :
1
Pages :
113-121
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
Bibliography
Benderitter M., Vincent-Genod L., Pouget J.P., and Voisin P. The cell membrane as a biosensor of oxidative stress induced by radiation exposure: a multiparameter investigation. Radiation Research 159 (2003) 471-483
Borst J.W., Visser N.V., Kouptsova O., and Visser A.J. Oxidation of unsaturated phospholipids in membrane bilayer mixtures is accompanied by membrane fluidity changes. Biochimica et Biophysica Acta 1487 (2000) 61-73
Calder P.C. n - 3 Fatty acids, inflammation, and immunity - relevance to post surgical and critically ill patients. Lipids 39 (2004) 1147-1161
Cazzola R., Russo-Volpe S., Cervato G., and Cestaro B. Biochemical assessments of oxidative stress, erythrocyte membrane fluidity and antioxidant status in professional soccer players and sedentary controls. European Journal of Clinical Investigation 33 (2003) 924-930
Cazzola R., Rondanelli M., Russo-Volpe S., Ferrari E., and Cestaro B. Decreased membrane fluidity and altered susceptibility to peroxidation and lipid composition in overweight and obese female erythrocytes. Journal of Lipid Research 45 (2004) 1846-1851
Clarkson P.M., and Thompson H.S. Antioxidants: what role do they play in physical activity and health?. American Journal of Clinical Nutrition 72 Suppl. 2 (2000) 637S-646S
Costabile M., Hii C.S., Melino M., Easton C., and Ferrante A. The immunomodulatory effects of novel beta-oxa, beta-thia, and gamma-thia polyunsaturated fatty acids on human T lymphocyte proliferation, cytokine production, and activation of protein kinase C and MAPKs. Journal of Immunology 174 (2005) 233-243
Crosby A.J., Wahle K.W.J., and Duthie G.G. Modulation of glutathione peroxidase activity in human vascular endothelial cells by fatty acids and the cytokine interleukin-1 beta. Biochimica et Biophysica Acta 1303 (1996) 187-192
D'Aquino M., Benedetti P.C., Di Felice M., Gentili V., Tomassi G., Maiorino M., and Ursini F. Effect of fish oil and coconut oil on antioxidant defence system and lipid peroxidation in rat liver. Free Radical Biology and Medicine 1 (1991) 147-152
de Moffarts B., Kirschvink N., Art T., Pincemail J., Michaux C., Cayeux K., Defraigne J.O., and Lekeux P. Impact of training and exercise intensity on blood antioxidant markers in healthy Standardbred horses. Equine and Comparative Exercise Physiology 1 (2004) 211-220
de Moffarts B., Kirschvink N., Art T., Pincemail J., and Lekeux P. Effect of oral antioxidant supplementation on blood antioxidant status in trained thoroughbred horses. The Veterinary Journal 169 (2005) 65-74
Deaton C.M., Marlin D.J., Roberts C.A., Smith N., Harris P.A., Kelly F.J., and Schroter R.C. Antioxidant supplementation and pulmonary function at rest and exercise. Equine Veterinary Journal Supplements 34 (2002) 58-65
Drieu K., Vranckx R., Benassayad C., Haourigi M., Hassid J., Yoa R.G., Rapin J.R., and Nunez E.A. Effect of the extract of Ginkgo biloba (EGb 761) on the circulating and cellular profiles of polyunsaturated fatty acids: correlation with the anti-oxidant properties of the extract. Prostaglandins Leukotrienes and Essential Fatty Acids 63 (2000) 293-300
Hall J.A., Van Saun R.J., and Wander R.C. Dietary (n - 3) fatty acids from menhaden fish oil alter plasma fatty acids and leukotriene B synthesis in healthy horses. Journal of Veterinary Internal Medicine 18 (2004) 871-879
Hall J.A., Van Saun R.J., Tornquist S.J., Gradin J.L., Pearson E.G., and Wander R.C. Effect of type of dietary polyunsaturated fatty acid supplement (corn oil or fish oil) on immune responses in healthy horses. Journal of Veterinary Internal Medicine 18 (2004) 880-886
Hargreaves B.J., Kronfeld D.S., Waldron J.N., Lopes M.A., Gay L.S., Saker K.E., Cooper W.L., Sklan D.J., and Harris P.A. Antioxidant status and muscle cell leakage during endurance exercise. Equine Veterinary Journal Supplements 34 (2002) 116-121
Hildebrandt W., Alexander S., Bartsch P., and Droge W. Effect of N-acetyl-cysteine on the hypoxic ventilatory response and erythropoietin production: linkage between plasma thiol redox state and O(2) chemosensitivity. Blood 99 (2002) 1552-1555
Hinchcliff K.W., Reinhart G.A., DiSilvestro R., Reynolds A., Blostein-Fujii A., and Swenson R.A. Oxidant stress in sled dogs subjected to repetitive endurance exercise. American Journal of Veterinary Research 61 (2000) 512-517
Hollan S. Membrane fluidity of blood cells. Haematologia (Budapest) 27 (1996) 109-127
Keddad K., Therond P., Motta C., Baussan C., and Legrand A. Alterations in erythrocyte membrane fluidity and fatty acid composition in glycogen storage disease. Biochimica et Biophysica Acta 1315 (1996) 61-65
Keith A.D., Sharnoff M., and Cohn G.E. A summary and evaluation of spin labels used as probes for biological membrane structure. Biochimica et Biophysica Acta 300 (1973) 379-419
Leibovitz B.E., Hu M.L., and Tappel A.L. Lipid peroxidation in rat tissue slices: effect of dietary Vit E, corn oil-lard and menhaden oil. Lipids 25 (1990) 125-129
Lemaître D., Vericel E., Polette A., and Lagarde M. Effects of fatty acids on human platelet glutathione peroxidase: possible role of oxidative stress. Biochemical Pharmacology 53 (1997) 479-486
Ma D.W., Seo J., Switzer K.C., Fan Y.Y., McMurray D.N., Lupton J.R., and Chapkin R.S. n - 3 PUFA and membrane microdomains: a new frontier in bioactive lipid research. Journal of Nutritional Biochemistry 15 (2004) 700-706
Marlin D.J., Fenn K., Smith N., Deaton C.D., Roberts C.A., Harris P.A., Dunster C., and Kelly F.J. Changes in circulatory antioxidant status in horses during prolonged exercise. Journal of Nutrition 132 Suppl. 2 (2002) 1622S-1627S
Mills P.C., Smith N.C., Casas I., Harris P., Harris R.C., and Marlin D.J. Effects of exercise intensity and environmental stress on indices of oxidative stress and iron homeostasis during exercise in the horse. European Journal of Applied Physiology and Occupational Physiology 74 (1996) 60-66
Mueller B.A., and Talbert R.L. Biological mechanisms and cardiovascular effects of omega-3 fatty acids. Clinical Pharmacology 7 (1988) 795-807
Nieman D.C. Immune response to heavy exertion. Journal of Applied Physiology 82 (1997) 1385-1394
O'Connor C.I., Lawrence L.M., Lawrence A.C., Janicki K.M., Warren L.K., and Hayes S. The effect of dietary fish oil supplementation on exercising horses. Journal of Animal Sciences 82 (2004) 2978-2984
Ochoa J.J., Vilchez M.J., Palacios M.A., Garcia J.J., Reiter R.J., and Munoz-Hoyos A. Melatonin protects against lipid peroxidation and membrane rigidity in erythrocytes from patients undergoing cardiopulmonary bypass surgery. Journal of Pineal Research 35 (2003) 104-108
Ojuawo A., and Keith L. The serum concentrations of zinc, copper and selenium in children with inflammatory bowel disease. Cent Africa Journal of Medicine 48 (2002) 116-119
O'Neill W., McKee S., and Clarke A.F. Flaxseed (Linum usitatissimum) supplementation associated with reduced skin test lesional area in horses with Culicoides hypersensitivity. Canadian Journal of Veterinary Research 66 (2002) 272-277
Palozza P., Sgarlata E., Luberto C., Piccioni E., Anti M., Marra G., Armelao F., Franceschelli P., and Bartoli G.M. N - 3 fatty acids induce oxidative modifications in human erythrocytes depending on dose and duration of dietary supplementation. American Journal of Clinical Nutrition 64 (1996) 297-304
Reznick A.Z., and Packer L. Oxidative damage to proteins: spectrophotometric method for carbonyl assay. Methods in Enzymology 233 (1994) 357-363
Schmidt E.B., Arnesen H., de Caterina R., Rasmussen L.H., and Kristensen S.D. Marine n - 3 polyunsaturated fatty acid and coronary disease. Thrombosis Research 115 (2005) 163-170
Sen C.K., and Packer L. Thiol homeostasis and supplements in physical exercise. American Journal of Clinical Nutrition 72 Suppl. 2 (2000) 653S-669S
Senturk U.K., Yalcin O., Gunduz F., Kuru O., Meiselman H.J., and Baskurt O.K. Effect of antioxidant vitamin supplement on the time course of hematological and hemorheological alterations after an exhausting exercise episode in human subjects. Journal of Applied Physiology 98 (2005) 1272-1279
Song J.H., Fujimoto K., and Miyazawa T. Polyunsaturated (n - 3) fatty acids susceptible to peroxidation are increased in plasma and tissue lipids of rats fed docosahexaenoic acid-containing oils. Journal of Nutrition 130 (2000) 3028-3033
Venkatraman J.T., Chandrasekar B., Kim J.D., and Fernandes G. Effects of n - 3 and n - 6 fatty acids on the activities and expression of hepatic antioxidant enzymes in autoimmune-prone NZB × NZW F1 mice. Lipids 29 (1994) 561-568
Similar publications
Sorry the service is unavailable at the moment. Please try again later.
This website uses cookies to improve user experience. Read more
Save & Close
Accept all
Decline all
Show detailsHide details
Cookie declaration
About cookies
Strictly necessary
Performance
Strictly necessary cookies allow core website functionality such as user login and account management. The website cannot be used properly without strictly necessary cookies.
This cookie is used by Cookie-Script.com service to remember visitor cookie consent preferences. It is necessary for Cookie-Script.com cookie banner to work properly.
Performance cookies are used to see how visitors use the website, eg. analytics cookies. Those cookies cannot be used to directly identify a certain visitor.
Used to store the attribution information, the referrer initially used to visit the website
Cookies are small text files that are placed on your computer by websites that you visit. Websites use cookies to help users navigate efficiently and perform certain functions. Cookies that are required for the website to operate properly are allowed to be set without your permission. All other cookies need to be approved before they can be set in the browser.
You can change your consent to cookie usage at any time on our Privacy Policy page.
