Beta-lactamase of Bacillus licheniformis 749/C at 2 A resolution.

Bacillus/enzymology/genetics; Bacterial Proteins/genetics/metabolism; Binding Sites; Models, Molecular; Penicillinase/genetics/metabolism; Penicillins/metabolism; Protein Binding; Protein Conformation; X-Ray Diffraction

Abstract :

[en] Two crystal forms (A and B) of the 29,500 Da Class A beta-lactamase (penicillinase) from Bacillus licheniformis 749/C have been examined crystallographically. The structure of B-form crystals has been solved to 2 A resolution, the starting model for which was a 3.5 A structure obtained from A-form crystals. The beta-lactamase has an alpha + beta structure with 11 helices and 5 beta-strands seen also in a penicillin target DD-peptidase of Streptomyces R61. Atomic parameters of the two molecules in the asymmetric unit were refined by simulated annealing at 2.0 A resolution. The R factor is 0.208 for the 27,330 data greater than 3 sigma (F), with water molecules excluded from the model. The catalytic Ser-70 is at the N-terminus of a helix and is within hydrogen bonding distance of conserved Lys-73. Also interacting with the Lys-73 are Asn-132 and the conserved Glu-166, which is on a potentially flexible helix-containing loop. The structure suggests the binding of beta-lactam substrates is facilitated by interactions with Lys-234, Thr-235, and Ala-237 in a conserved beta-strand peptide, which is antiparallel to the beta-lactam's acylamido linkage; an exposed cavity near Asn-170 exists for acylamido substituents. The reactive double bond of clavulanate-type inhibitors may interact with Arg-244 on the fourth beta-strand. A very similar binding site architecture is seen in the DD-peptidase.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Moews, P. C.

Knox, J. R.

Dideberg, O.

Charlier, Paulette ; Université de Liège - ULiège > Département des sciences de la vie > Cristallographie des macromolécules biologiques

Frère, Jean-Marie ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Language :

English

Title :

Beta-lactamase of Bacillus licheniformis 749/C at 2 A resolution.

Publication date :

1990

Journal title :

Proteins

ISSN :

0887-3585

eISSN :

1097-0134

Publisher :

Wiley Liss, Inc., New York, United States - New York

Volume :

Issue :

Pages :

156-71

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 29 November 2010

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