Epidemiological Evaluation of a Monoclonal Elisa Detecting Bovine Viral Diarrhoea Pestivirus Antigens in Field Blood Samples of Persistently Infected Cattle
[en] An enzyme-linked immunosorbent assay (ELISA), using monoclonal antibodies for capture and detection, was developed for detecting bovine viral diarrhoea virus (BVDV) antigens in blood samples. The test was evaluated using 761 field samples of known status (viraemic or not). When an appropriate cut-off value was chosen, the sensitivity, specificity, and predictive values of the assay were 100%, higher than the values obtained by classical virus isolation. Correlation with the latter technique exceeded 90%. The ELISA is a good candidate for replacing virus isolation as a reference method for BVDV antigen detection in persistently infected carriers. A method based on the mean of the standard deviation ratio can be used to choose the cut-off value in order to optimise reproducibility.
Disciplines :
Microbiology Veterinary medicine & animal health
Author, co-author :
Mignon, Bernard ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires
Waxweiler, Sophie
Thiry, Etienne ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Virologie, épidémiologie et pathologie des maladies virales
Boulanger, D.
Dubuisson, J.
Pastoret, Paul-Pierre ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Département des maladies infectieuses et parasitaires
Language :
English
Title :
Epidemiological Evaluation of a Monoclonal Elisa Detecting Bovine Viral Diarrhoea Pestivirus Antigens in Field Blood Samples of Persistently Infected Cattle
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Bibliography
Bennet, Done (1986) Control of the bovine pestivirus syndrome in cattle: a case for social cost-benefit analysis?. Proc. Soc. Vet. Epid. Prevent. Med., Thrusfield, Edimbourg; .
Bielefeldt, Rønsholt, Bloeh (1987) Demonstration of bovine viral diarrhoea virus in peripheral blood mononuclear cells of persistently infected, clinically normal cattle. J. Gen. Virol. 68:1971-1982.
Boulanger, Waxweiler, Karelle, Loncar, Mignon, Dubuisson, Thiry, Pastoret (1991) Characterization of monoclonal antibodies to bovine viral diarrhoea virus: evidence of a neutralizing activity against gp48 in the presence of goat anti-mouse immunoglobulin serum. J. Gen. Virol. 72:1195-1198.
Brownlie (1990) Pathogenesis of mucosal disease and molecular aspects of bovine virus diarrhoea virus. Vet. Microbiol. 23:371-382.
Brownlie, Clarke, Howard, Pocock (1987) Pathogenesis and epidemiology of bovine virus diarrhoea virus infection of cattle. Ann. Rech. Vet. 128:157-166.
Collet, Moennig, Horzinek (1989) Recent advances in Pestivirus research. J. Gen. Virol. 70:253-266.
Donis, Dubovi (1987) Differences in virus-induced polypeptides in cells infected by cytopathic and non-cytopathic biotypes of bovine virus diarrhoea-mucosal disease virus. Virology 158:168-173.
Fenton, Entrican, Herring, Nettleton (1990) An ELISA for detecting pestivirus antigen in the blood of sheep persistently infected with border disease virus. J. Virol. Methods 27:253-260.
Martin, Meek, Willeberg Veterinary Epidemiology. Principles and methods, Iowa State University Press; 1987.
Meyling (1984) Detection of the BVD virus in viraemic cattle by an indirect immunoperoxidase technique. Recent Advances in Virus diagnosis , M.S. McNulty, J.B. McFerran, Martinus Nijhoff, Boston; 37-46.
Mignon, Dubuisson, Baranowski, Koromyslov, Ernst, Boulanger, Waxweiler, Pastoret (1991) A monoclonal ELISA for bovine diarrhoea pestivirus antigen detection in persistently infected cattle. J. Virol. Methods 35:177-188.
Parkinson, Conradie, Milner, Marimuthu (1988) The interpretation of ELISA results by means of the standard deviation ratio. J. Immunol. Methods 115:105-110.
Shannon, Richards, Kirkland, Moyle (1991) An antigen-capture ELISA detects pestivirus antigens in blood and tissues of immunotolerant carrier cattle. J. Virol. Methods 34:1-12.
Waxweiler, Karelle-Bui-Thi, Sneyers, Lambert, Delchambre, Dubuisson, Thiry, Antoine, Dive, Detal, Pastoret (1989) Circulation de souches non-cytopathogènes du virus BVD-MD dans des lots de taurillons. Ann. Méd. Vét. 133:681-690.
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