Epidemiological Evaluation of a Monoclonal Elisa Detecting Bovine Viral Diarrhoea Pestivirus Antigens in Field Blood Samples of Persistently Infected Cattle

Mignon, Bernard; Waxweiler, Sophie; Thiry, Etienne; Boulanger, D.; Dubuisson, J.; Pastoret, Paul-Pierre

doi:10.1016/0166-0934(92)90010-B

Article (Scientific journals)

Epidemiological Evaluation of a Monoclonal Elisa Detecting Bovine Viral Diarrhoea Pestivirus Antigens in Field Blood Samples of Persistently Infected Cattle

Mignon, Bernard; Waxweiler, Sophie; Thiry, Etienne et al.

1992 • In Journal of Virological Methods, 40 (1), p. 85-93

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https://hdl.handle.net/2268/7760

DOI
10.1016/0166-0934(92)90010-B

PubMed
1331162

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Abstract :

[en] An enzyme-linked immunosorbent assay (ELISA), using monoclonal antibodies for capture and detection, was developed for detecting bovine viral diarrhoea virus (BVDV) antigens in blood samples. The test was evaluated using 761 field samples of known status (viraemic or not). When an appropriate cut-off value was chosen, the sensitivity, specificity, and predictive values of the assay were 100%, higher than the values obtained by classical virus isolation. Correlation with the latter technique exceeded 90%. The ELISA is a good candidate for replacing virus isolation as a reference method for BVDV antigen detection in persistently infected carriers. A method based on the mean of the standard deviation ratio can be used to choose the cut-off value in order to optimise reproducibility.

Disciplines :

Microbiology
Veterinary medicine & animal health

Author, co-author :

Mignon, Bernard ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires

Waxweiler, Sophie

Thiry, Etienne ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Virologie, épidémiologie et pathologie des maladies virales

Boulanger, D.

Dubuisson, J.

Pastoret, Paul-Pierre ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Département des maladies infectieuses et parasitaires

Language :

English

Title :

Epidemiological Evaluation of a Monoclonal Elisa Detecting Bovine Viral Diarrhoea Pestivirus Antigens in Field Blood Samples of Persistently Infected Cattle

Publication date :

October 1992

Journal title :

Journal of Virological Methods

ISSN :

0166-0934

eISSN :

1879-0984

Publisher :

Elsevier, Netherlands

Volume :

Issue :

Pages :

85-93

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 05 March 2009

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Bibliography

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