[en] In species of great conservation concern, special attention must be paid to their phylogeography, in particular the origin of animals for captive breeding and reintroduction. The endangered European mink lives now in at least three well-separated populations in northeast, southeast and west Europe. Our aim is to assess the genetic structure of these populations to identify 'distinct population segments' (DPS) and advise captive breeding programmes. First, the mtDNA control region was completely sequenced in 176 minks and 10 polecats. The analysis revealed that the western population is characterized by a single mtDNA haplotype that is closely related to those in eastern regions but nevertheless, not found there to date. The northeast European animals are much more variable (pi = 0.012, h = 0.939), with the southeast samples intermediate (pi = 0.0012, h = 0.469). Second, 155 European mink were genotyped using six microsatellites. The latter display the same trends of genetic diversity among regions as mtDNA [gene diversity and allelic richness highest in northeast Europe (H(E) = 0.539, R(S) = 3.76), lowest in west Europe (H(E) = 0.379, R(S) = 2.12)], and provide evidences that the southeast and possibly the west populations have undergone a recent bottleneck. Our results indicate that the western population derives from a few animals which recently colonized this region, possibly after a human introduction. Microsatellite data also reveal that isolation by distance occurs in the western population, causing some inbreeding because related individuals mate. As genetic data indicate that the three populations have not undergone independent evolutionary histories for long (no phylogeographical structure), they should not be considered as distinct DPS. In conclusion, the captive breeding programme should use animals from different parts of the species' present distribution area.
Disciplines :
Zoology
Author, co-author :
Michaux, Johan ; Université de Liège - ULiège > Département des sciences de la vie > Génétique
Hardy, O. J.
Justy, F.
Fournier, P.
Kranz, A.
Cabria, M.
Davison, A.
Rosoux, R.
Libois, Roland ; Université de Liège - ULiège > Département des sciences et gestion de l'environnement > Zoogéographie - Département des sciences et gestion de l'environnement
Language :
English
Title :
Conservation genetics and population history of the threatened European mink Mustela lutreola, with an emphasis on the west European population.
Aguilar A, Roemer G, Debenham S, Binns M, Garcelon D, Wayne E (2004) High MHC diversity maintained by balancing selection in an otherwise genetically monomorphic mammal. Proceedings of the National Academy of Sciences, USA, 101, 3490-3494.
Anonymous (1999) Plan de restauration du vison d'Europe en France. Ministère de l'Aménagement du Territoire et de l'Environnement, Paris, p. 57.
Avise JC (2000) Phylogeography: The History and Formation of Species. Harvard University Press, Cambridge, Massachusetts.
Baillie N, Groombridge C (1996) 1996 IUCN Red List of Threatened Animals. International Union for Conservation of Nature and Natural Resources, Gland, Switzerland.
Balakrishnan CN, Monfort SL, Gaur A, Singh L, Sorenson MD (2003) Phylogeography and conservation genetics of Eld's deer (Cervus eldi). Molecular Ecology, 12, 1-10.
de Bellefroid MN (1999) Etude biogéographique de l'évolution de la population de vison européen, Mustela lutreola, en France. PhD Thesis, University of Rennes.
de Bellefroid MN, Rosoux R (1998) Le 'vison du Poitou', un hôte des zones humides menacé dans le Centre-Ouest atlantique. Annales Société Sciences Naturelles Charerite-Maritime, 8, 865-879.
Brunhoff C, Galbreath KE, Fedorov VB, Cook JA, Jaarola M (2003) Holarctic phylogeography of the root vole (Microtus oeconomus): implications for late Quaternary biogeography of high latitudes. Molecular Ecology, 12, 957-968.
Burban C, Petit RJ, Carcreff E, Jactel H (1999) Rangewide variation of the marine pine bast scale Matsucoccus Teytoudi Duc. (Homoptera: Matsuccidae) in relation to the genetic structure of its host. Molecular Ecology, 8, 1593-1602.
Camby A (1990) Le Vison d'Europe (Mustela lutreola (Linnaeus, 1761)). Encyclopédie des Carnivores de France, no. 13. Société française pour l'Etude et la Protection des Mammifères, Nort-sur-Erdre.
Carr SM, Hick SA (1997) Are there two species of martin in North America? Genetic and evolutionary relationships within Martes. In: Martes, Taxonomys, Ecology, Techniques and Management (eds Proulx G, Bryant HN, Woodard PM), pp. 15-28. Provincial Museum of Alberta, Edmonton, Alberta.
Cassens I, Tiedemann R, Suchentrunk F, Hartl GB (2000) Mitochondrial DNA variation in the European otter (Lutra lutra) and the use of spatial autocorrelation analysis in conservation. Journal of Heredity, 91, 31-35.
Cavalli-Sforza LL, Edwards AWF (1967) Phylogenetic analysis: models and estimation procedures. American Journal of Human Genetics, 19, 233-257.
Clement M, Posada D, Crandall KA (2000) TCS: a computer program to estimate gene genealogies. Molecular Ecology, 9, 1657-1659.
Cornuet JM, Luikart G (1996) Description and power analysis of two tests for detecting recent population bottlenecks from allele frequency data. Genetics, 144, 2001-2014.
Crandall KA, Bininda-Edmonds ORP, Mace GM, Wayne RK (2000) Considering evolutionary processes in conservation biology. Trends in Ecology & Evolution, 15, 290-295.
Davis CS, Strobeck C (1998) Isolation, variability, and cross-species amplification of polymorphic microsatellite loci in the family Mustelidae. Molecular Ecology, 7, 1776-1778.
Davison A, Birks JD, Brookes RC, Messenger JE, Griffiths HI (2001) Mitochondrial phylogeography and population history of pine martens Martes martes compared with polecats Mustela putorius. Molecular Ecology, 10, 2479-2488.
Davison A, Griffiths HI, Brookes RC et al. (2000) Mitochondrial DNA and palaeontological evidence for the origins of the endangered European mink, Mustela lutreola. Animal Conservation, 3, 345-355.
Drew RE, Hallett JG, Aubry KB, Cullings KW, Koepf SM, Zielinski WJ (2003) Conservation genetics of the fisher (Martes pennanti) based on mitochondrial DNA sequencing. Molecular Ecology, 12, 51-62.
Ellegren H, Hartman G, Johansson M, Andersson L (1993) Major histocompatibility complex monomorphism and low levels of DNA fingerprinting variability in a reintroduced and rapidly expanding population of beavers. Proceedings of the National Academy of Sciences, USA, 90, 8150-8153.
Ellegren H, Mikko S, Wallin K, Andersson L (1996) Limited polymorphism at major histocompatibility complex (MHC) loci in the Swedish moose A. alces. Molecular Ecology, 5, 3-9.
Fitch WM (1971) Towards defining the course of evolution: Minimum change for a specific tree topology. Systematic Zoology, 20, 406-416.
Fleming MA, J.Cook (2002) Phylogeography of endemic ermine (Mustela erminea) in southeast Alaska. Molecular Ecology, 11, 795-807.
Fleming MA, Ostrander EA, Cook JA (1999) Microsatellite markers for American mink (Mustela vison) and ermine (Mustela erminea). Molecular Ecology, 8, 1351-1352.
Fournier-Chambrillon C, Aasted B, Perrot A et al. (2004) Antibodies to Aleutian mink disease parvovirus in free-ranging European mink (Mustela lutreola) and other small carnivores from southwestern France. Journal of Wildlife Diseases, 40, in press.
Fraser DJ, Bernatchez L (2001) Adaptive evolutionary conservation: towards a unified concept for defining conservation units. Molecular Ecology, 10, 2741-2752.
Gascuel O (1997) BIONJ: an improved version of the NJ algorithm based on a simple model sequence of data. Molecular Biology and Evolution, 14, 685-695.
Gotea V, Kranz A (1999) The European mink (Mustela lutreola) in the Danube delta. Small Carnivore Conservation, 21, 23-25.
Goudet J (1995) FSTAT version 1.2: a computer program to calculate F-statistics. Journal of Heredity, 86, 485-486.
Hardy OJ, Vekemans X (2002) SPAGEDI: a versatile computer program to analyse spatial genetic structure at the individual or population levels. Molecular Ecology Notes, 2, 618-620.
Hewitt GM (1996) Some genetic consequences of ice ages and their role in divergence and speciation. Biological Journal of the Linnean Society of, 58, 247-276.
Hewitt GM (2004) Genetic consequences of climatic oscillations in the Quaternary. Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences, 359, 183-195.
Huelsenbeck JP, Ronquist F, Nielsen R, Bollback JP (2001) Bayesian inference of phylogeny and its impact on evolutionary biology. Science, 294, 2310-2314.
Ibrahim KM, Nichols RA, Hewitt GM (1996) Spatial patterns of genetic variation generated by different forms of dispersal during range expansion. Heredity, 77, 282-291.
Loiselle BA, Sork VL, Nason J, Graham C (1995) Spatial genetic structure of a tropical understorey shrub, Psychotria officinalis (Rubiaceae). American Journal of Botany, 82, 1420-1425.
Lynch M (1991) The genetic interpretation of inbreeding depression and outbreeding depression. Evolution, 45, 622-629.
Maizeret C, Migot P, Rosoux R et al. (2002) The distribution of the European mink (Mustela lutreola) in France: towards a short term extinction? Mammalia, 66, 525-532.
Maran T (1992) The European mink, Mustela lutreola, in protected areas in the former Soviet Union. Small Carnivore Conservation, 7, 10-12.
Merilae J, Bjoerklund M, Baker AJ (1997) Historical demography and present day population structure of the greenfinch, Carduelis chloris. An analysis of mtDNA control-region sequences. Evolution, 51, 946-956.
Michaux JR, Magnanou E, Paradis E, Nieberding C, Libois RM (2003) Mitochondrial phylogeography of the woodmouse (Apodemus sylvaticus) in the western Palearctic region. Molecular Ecology, 12, 685-697.
Michaux JR, Filippucci MG, Libois R (2004a) Phylogeographic history of the yellow-necked fieldmouse (Apodemus flavicollis) in Europe and in the Near and Middle East. Molecular Phylogenetics and Evolution, 32, 788-798.
Michaux JR, Libois RM, Davison A, Rosoux R (2004b) Are French and Spanish European mink, Mustela lutreola, a distinct management unit for conservation? Biological Conservation, 115, 357-367.
Mikko S, Andersson L (1995) Low major histocompatibility complex class II diversity in European and North American moose. Proceedings of the National Academy of Sciences, USA, 92, 4259-4263.
Morales CF (2002) Phylogéographie de la loutre d'Europe (Lutra lutra) dans la partie occidentale de son aire de répartition. DES Sciences Pays en Développement Thesis, University of Liège.
Moritz C (1994) Applications of mitochondrial DNA analysis in conservation: a critical review. Molecular Ecology, 3, 401-411.
Philippe H (1993) MUST, a computer package for management utilities for sequences and trees. Nucleic Acids Research, 21, 5264-5272.
Pons O, Petit RJ (1996) Measuring and testing genetic differentiation with ordered versus unordered alleles. Genetics, 144, 1237-1245.
Posada D, Crandall A (1998) MODELTEST: testing then model of DNA substitution. Bioinformatics, 14, 817-818.
Randi E, Davoli F, Pierpaoli M, Pertoldi C, Madsen AB, Loeschke V (2003) Genetic structure in otter (Lutra lutra) populations in Europe: implications for conservation. Animal Conservation, 6, 1-10.
Rozas J, Rozas R (1997) DNASP, version 2.0: a novel software package for extensive molecular population genetic analysis. Computer Application in the Biosciences, 13, 307-311.
Ruiz-Olmo J, Palazón S (1991) New information on the European and American mink in the Iberian Peninsula. Mustelid Viverrid Conservation, 5, 13.
Ryder OA (1986) Species conservation and systematics: the dilemma of subspecies. Trends in Ecology and Evolution, 1, 9-10.
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4, 406-425.
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
Schneider S, Roessli D, Excoffier L (2000) ARLEQUIN: a software for population genetic data analysis version 2.0. Genetics and Biometry Laboratory. University of Geneva, Switzerland.
Sidorovich VE (2000) The ongoing decline of riparian mustelids (European mink, Mustela lutreola, polecat, Mustela putorius and stoat, Mustela erminea) in eastern Europe: a review of the results to date and a hypothesis. In: The Mustelids in a Modern World Management and Conservation Aspects of Small Carnivore: Human Interactions (ed. Griffiths HI), pp. 295-317. Backhuys Press, Leiden.
Spielman D, Brook BW, Frankham R (2004) Most species are not driven to extinction before genetic factors impact them. Proceedings of the National Academy of Sciences, USA, 101, 15261-15264.
Swofford DL (1998) PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods), Version 4 0b. Sinauer Associates, Sunderland, Massachusetts.
Taberlet P, Fumagalli L, Wust-Saucy AG, Cosson JF (1998) Comparative phylogeography and postglacial colonization routes in Europe. Molecular Ecology, 7, 453-464.
Tarr CL, Fleischer RC (1999) Population boundaries and genetic diversity in the endangered Mariana crow (Corvus kubaryi). Molecular Ecology, 8, 941-949.
Tumanov IL (1992) The number of European mink (Mustela lutreola) in the eastern area and its relation to American mink. In: Semi-Aquatische Saügetiere Materialen Des 2 Internat Symposium (eds Schröpfer R et al.), pp. 329-336, Martin Lüther Universität, Halle-Wittenberg.
US Fish and Wildlife Service and National Fisheries Service (1996) Policy regarding the recognition of distinct vertebrate population segments under the Endangered Species. Act Federal Register, 61, 4721-4725.
Van Bree PJH, Saint Girons MC (1966) Données sur la répartition et la taxonomie de Mustela lutreola (Linnaeus, 1761) en France. Mammalia, 30, 270-291.
Vekemans X, Hardy OJ (2004) New insights from fine-scale spatial genetic structure analyses in plant populations. Molecular Ecology, in press.
Vila C, Amorim IR, Leonard JA et al. (1999) Mitochondrial DNA phylogeography and population history of the grey wolf Canis lupus. Molecular Ecology, 8, 2089-2103.
Waits LP, Talbot S, Ward RH, Shields GF (1998) Mitochondrial DNA phylogeography of the North American brown bear and implications for conservation. Conservation Biology, 12, 408-417.
Walker WK, Vila C, Landa A, Linden M, Ellegren H (2001) Genetic variation and population structure in Scandinavian wolverine (Gulo gulo) populations. Molecular Ecology, 10, 53-63.
Weir BS, Cockerham CC (1984) Estimating F-statistics for the analysis of population structure. Evolution, 38, 1358-1370.
Wright S (1992) Coefficients of inbreeding and relationships. American Naturalist, 56, 330-338.
Yang Z (1996) Among-site rate variation and its impact on phylogenetic analyses. Trends Ecology & Evolution, 11, 367-372.
Yang Z, Rannala B (1997) Bayesian phylogenetic inference using DNA sequences: a Markov chain Montecarlo method. Molecular Biology and Evolution, 14, 717-724.
Youngman PM (1982) Distribution and systematics of the European mink, Mustela lutreola. Acta Zoologica Fennica, 166, 1-48.